Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium
Summary: The clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether similar processes exist in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of PIWI-clade Argonautes involve...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723002243 |
| _version_ | 1811160181355053056 |
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| author | Therese Solberg Victor Mason Chundi Wang Mariusz Nowacki |
| author_facet | Therese Solberg Victor Mason Chundi Wang Mariusz Nowacki |
| author_sort | Therese Solberg |
| collection | DOAJ |
| description | Summary: The clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether similar processes exist in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of PIWI-clade Argonautes involved in various small RNA (sRNA) pathways, many of which have not yet been investigated. Here, we investigate the function of a PIWI protein, Ptiwi08, whose expression is limited to a narrow time window during development, concomitant with the start of zygotic transcription. We show that Ptiwi08 acts in an endogenous small interfering RNA (endo-siRNA) pathway involved in the clearance of untranslated mRNAs. These endo-siRNAs are found in clusters that are strictly antisense to their target mRNAs and are a subset of siRNA-producing clusters (SRCs). Furthermore, the endo-siRNAs are 2′-O-methylated by Hen1 and require Dcr1 for their biogenesis. Our findings suggest that sRNA-mediated developmental mRNA clearance extends beyond metazoans and may be a more widespread mechanism than previously anticipated. |
| first_indexed | 2024-04-10T05:53:16Z |
| format | Article |
| id | doaj.art-c8b35dec75204a1090250b8e330b42ac |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-10T05:53:16Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-c8b35dec75204a1090250b8e330b42ac2023-03-04T04:22:56ZengElsevierCell Reports2211-12472023-03-01423112213Developmental mRNA clearance by PIWI-bound endo-siRNAs in ParameciumTherese Solberg0Victor Mason1Chundi Wang2Mariusz Nowacki3Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, SwitzerlandInstitute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, SwitzerlandInstitute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, ChinaInstitute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland; Corresponding authorSummary: The clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether similar processes exist in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of PIWI-clade Argonautes involved in various small RNA (sRNA) pathways, many of which have not yet been investigated. Here, we investigate the function of a PIWI protein, Ptiwi08, whose expression is limited to a narrow time window during development, concomitant with the start of zygotic transcription. We show that Ptiwi08 acts in an endogenous small interfering RNA (endo-siRNA) pathway involved in the clearance of untranslated mRNAs. These endo-siRNAs are found in clusters that are strictly antisense to their target mRNAs and are a subset of siRNA-producing clusters (SRCs). Furthermore, the endo-siRNAs are 2′-O-methylated by Hen1 and require Dcr1 for their biogenesis. Our findings suggest that sRNA-mediated developmental mRNA clearance extends beyond metazoans and may be a more widespread mechanism than previously anticipated.http://www.sciencedirect.com/science/article/pii/S2211124723002243CP: Molecular biologyCP: Cell biology |
| spellingShingle | Therese Solberg Victor Mason Chundi Wang Mariusz Nowacki Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium Cell Reports CP: Molecular biology CP: Cell biology |
| title | Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium |
| title_full | Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium |
| title_fullStr | Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium |
| title_full_unstemmed | Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium |
| title_short | Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium |
| title_sort | developmental mrna clearance by piwi bound endo sirnas in paramecium |
| topic | CP: Molecular biology CP: Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723002243 |
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