Rhizosphere Bacteria From Panax notoginseng Against Meloidogyne hapla by Rapid Colonization and Mediated Resistance
Root-knot nematodes (RKNs) are soil-borne pathogens that severely affect Panax notoginseng growth and productivity. Thus, there is an urgent need for biological control agents or green nematicides to control root-knot nematodes. Rhizosphere bacteria can effectively control RKNs through different mec...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.877082/full |
| _version_ | 1818011095458119680 |
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| author | Wentao Wu Jingjing Wang Zhuhua Wang Liwei Guo Shusheng Zhu Youyong Zhu Yang Wang Xiahong He Xiahong He |
| author_facet | Wentao Wu Jingjing Wang Zhuhua Wang Liwei Guo Shusheng Zhu Youyong Zhu Yang Wang Xiahong He Xiahong He |
| author_sort | Wentao Wu |
| collection | DOAJ |
| description | Root-knot nematodes (RKNs) are soil-borne pathogens that severely affect Panax notoginseng growth and productivity. Thus, there is an urgent need for biological control agents or green nematicides to control root-knot nematodes. Rhizosphere bacteria can effectively control RKNs through different mechanisms. In this study, the three rhizosphere Bacillus strains, isolated from the root of P. notoginseng, were evaluated for the nematicidal activity and biological control efficacy against root-knot nematodes. In addition, we also evaluated the colonization ability of the two bacterial strains with significant biocontrol effect and dynamic regulation of genes related to systemic resistance in P. notoginseng. The rhizosphere Bacillus velezensis GJ-7 and Bacillus cereus NS-2 showed high nematicidal activity against Meloidogyne hapla in vitro and significantly reduced the number of root galls in three different control experiments. The results of colonization experiments showed that the strains GJ-7 and NS-2 colonized P. notoginseng root rapidly and stably. Additionally, the colonization of the strains NS-2 and GJ-7 activated the defense-responsive genes in P. notoginseng. These results indicated that the B. cereus strain NS-2 and B. velezensis strain GJ-7 have the potential for successful ecological niche occupation and enhance plant resistance and therefore could be considered as potential biocontrol agents against root-knot nematodes. |
| first_indexed | 2024-04-14T06:03:01Z |
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| id | doaj.art-c8b3c3a853684a60ab7cabbffedfa913 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T06:03:01Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c8b3c3a853684a60ab7cabbffedfa9132022-12-22T02:08:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.877082877082Rhizosphere Bacteria From Panax notoginseng Against Meloidogyne hapla by Rapid Colonization and Mediated ResistanceWentao Wu0Jingjing Wang1Zhuhua Wang2Liwei Guo3Shusheng Zhu4Youyong Zhu5Yang Wang6Xiahong He7Xiahong He8Key Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming, ChinaSchool of Landscape and Horticulture, Southwest Forestry University, Kunming, ChinaRoot-knot nematodes (RKNs) are soil-borne pathogens that severely affect Panax notoginseng growth and productivity. Thus, there is an urgent need for biological control agents or green nematicides to control root-knot nematodes. Rhizosphere bacteria can effectively control RKNs through different mechanisms. In this study, the three rhizosphere Bacillus strains, isolated from the root of P. notoginseng, were evaluated for the nematicidal activity and biological control efficacy against root-knot nematodes. In addition, we also evaluated the colonization ability of the two bacterial strains with significant biocontrol effect and dynamic regulation of genes related to systemic resistance in P. notoginseng. The rhizosphere Bacillus velezensis GJ-7 and Bacillus cereus NS-2 showed high nematicidal activity against Meloidogyne hapla in vitro and significantly reduced the number of root galls in three different control experiments. The results of colonization experiments showed that the strains GJ-7 and NS-2 colonized P. notoginseng root rapidly and stably. Additionally, the colonization of the strains NS-2 and GJ-7 activated the defense-responsive genes in P. notoginseng. These results indicated that the B. cereus strain NS-2 and B. velezensis strain GJ-7 have the potential for successful ecological niche occupation and enhance plant resistance and therefore could be considered as potential biocontrol agents against root-knot nematodes.https://www.frontiersin.org/articles/10.3389/fmicb.2022.877082/fullrhizosphere bacteriabiological controlMeloidogyne haplarapid colonizationresistance induction |
| spellingShingle | Wentao Wu Jingjing Wang Zhuhua Wang Liwei Guo Shusheng Zhu Youyong Zhu Yang Wang Xiahong He Xiahong He Rhizosphere Bacteria From Panax notoginseng Against Meloidogyne hapla by Rapid Colonization and Mediated Resistance Frontiers in Microbiology rhizosphere bacteria biological control Meloidogyne hapla rapid colonization resistance induction |
| title | Rhizosphere Bacteria From Panax notoginseng Against Meloidogyne hapla by Rapid Colonization and Mediated Resistance |
| title_full | Rhizosphere Bacteria From Panax notoginseng Against Meloidogyne hapla by Rapid Colonization and Mediated Resistance |
| title_fullStr | Rhizosphere Bacteria From Panax notoginseng Against Meloidogyne hapla by Rapid Colonization and Mediated Resistance |
| title_full_unstemmed | Rhizosphere Bacteria From Panax notoginseng Against Meloidogyne hapla by Rapid Colonization and Mediated Resistance |
| title_short | Rhizosphere Bacteria From Panax notoginseng Against Meloidogyne hapla by Rapid Colonization and Mediated Resistance |
| title_sort | rhizosphere bacteria from panax notoginseng against meloidogyne hapla by rapid colonization and mediated resistance |
| topic | rhizosphere bacteria biological control Meloidogyne hapla rapid colonization resistance induction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.877082/full |
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