MdbHLH130, an Apple bHLH Transcription Factor, Confers Water Stress Resistance by Regulating Stomatal Closure and ROS Homeostasis in Transgenic Tobacco
Drought is a major environmental factor that significantly limits crop yield and quality worldwide. Basic helix-loop-helix (bHLH) transcription factors have been reported to participate in the regulation of various abiotic stresses. In this study, a bHLH transcription factor in apple, MdbHLH130, whi...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-10-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.543696/full |
| _version_ | 1818257289255059456 |
|---|---|
| author | Qiang Zhao Zihao Fan Lina Qiu Qinqin Che Ting Wang Yuanyuan Li Yongzhang Wang |
| author_facet | Qiang Zhao Zihao Fan Lina Qiu Qinqin Che Ting Wang Yuanyuan Li Yongzhang Wang |
| author_sort | Qiang Zhao |
| collection | DOAJ |
| description | Drought is a major environmental factor that significantly limits crop yield and quality worldwide. Basic helix-loop-helix (bHLH) transcription factors have been reported to participate in the regulation of various abiotic stresses. In this study, a bHLH transcription factor in apple, MdbHLH130, which contains a highly conserved bHLH domain, was isolated and characterized. qRT-PCR and PMdbHLH130::GUS analyses showed that MdbHLH130 was notably induced in response to dehydration stress. Compared with the wild-type (WT), transgenic apple calli overexpressing MdbHLH130 displayed greater resistance to PEG6000 treatment. In contrast, the MdbHLH130-Anti lines were more sensitive to PEG6000 treatment than WT. Moreover, ectopic expression of MdbHLH130 in tobacco improved tolerance to water deficit stress, and plants exhibited higher germination rates and survival rates, longer roots, and lower ABA-induced stomatal closure and leaf water loss than the WT control. Furthermore, overexpression of MdbHLH130 in tobacco also led to lower electrolyte leakage, malondialdehyde contents, and reactive oxygen species (ROS) accumulation and upregulation of the expression of some ROS-scavenging and stress-responsive genes under water deficit stress. In addition, MdbHLH130 transgenic tobacco plants exhibited improved tolerance to oxidative stress compared with WT. In conclusion, these results indicate that MdbHLH130 acts as a positive regulator of water stress responses through modulating stomatal closure and ROS-scavenging in tobacco. |
| first_indexed | 2024-12-12T17:41:17Z |
| format | Article |
| id | doaj.art-c8b5c45a401542d89ef1e8febb4cfe5d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T17:41:17Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c8b5c45a401542d89ef1e8febb4cfe5d2022-12-22T00:17:04ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-10-011110.3389/fpls.2020.543696543696MdbHLH130, an Apple bHLH Transcription Factor, Confers Water Stress Resistance by Regulating Stomatal Closure and ROS Homeostasis in Transgenic TobaccoQiang Zhao0Zihao Fan1Lina Qiu2Qinqin Che3Ting Wang4Yuanyuan Li5Yongzhang Wang6Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture, Qingdao Agricultural University, Qingdao, ChinaShandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture, Qingdao Agricultural University, Qingdao, ChinaShandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture, Qingdao Agricultural University, Qingdao, ChinaShandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture, Qingdao Agricultural University, Qingdao, ChinaEditorial Office of YanTai Fruits, Yantai Academy of Agricultural Sciences, Yantai, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, ChinaShandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture, Qingdao Agricultural University, Qingdao, ChinaDrought is a major environmental factor that significantly limits crop yield and quality worldwide. Basic helix-loop-helix (bHLH) transcription factors have been reported to participate in the regulation of various abiotic stresses. In this study, a bHLH transcription factor in apple, MdbHLH130, which contains a highly conserved bHLH domain, was isolated and characterized. qRT-PCR and PMdbHLH130::GUS analyses showed that MdbHLH130 was notably induced in response to dehydration stress. Compared with the wild-type (WT), transgenic apple calli overexpressing MdbHLH130 displayed greater resistance to PEG6000 treatment. In contrast, the MdbHLH130-Anti lines were more sensitive to PEG6000 treatment than WT. Moreover, ectopic expression of MdbHLH130 in tobacco improved tolerance to water deficit stress, and plants exhibited higher germination rates and survival rates, longer roots, and lower ABA-induced stomatal closure and leaf water loss than the WT control. Furthermore, overexpression of MdbHLH130 in tobacco also led to lower electrolyte leakage, malondialdehyde contents, and reactive oxygen species (ROS) accumulation and upregulation of the expression of some ROS-scavenging and stress-responsive genes under water deficit stress. In addition, MdbHLH130 transgenic tobacco plants exhibited improved tolerance to oxidative stress compared with WT. In conclusion, these results indicate that MdbHLH130 acts as a positive regulator of water stress responses through modulating stomatal closure and ROS-scavenging in tobacco.https://www.frontiersin.org/article/10.3389/fpls.2020.543696/fullapplebHLH transcription factorMdbHLH130water stressstomatal closureROS-scavenging |
| spellingShingle | Qiang Zhao Zihao Fan Lina Qiu Qinqin Che Ting Wang Yuanyuan Li Yongzhang Wang MdbHLH130, an Apple bHLH Transcription Factor, Confers Water Stress Resistance by Regulating Stomatal Closure and ROS Homeostasis in Transgenic Tobacco Frontiers in Plant Science apple bHLH transcription factor MdbHLH130 water stress stomatal closure ROS-scavenging |
| title | MdbHLH130, an Apple bHLH Transcription Factor, Confers Water Stress Resistance by Regulating Stomatal Closure and ROS Homeostasis in Transgenic Tobacco |
| title_full | MdbHLH130, an Apple bHLH Transcription Factor, Confers Water Stress Resistance by Regulating Stomatal Closure and ROS Homeostasis in Transgenic Tobacco |
| title_fullStr | MdbHLH130, an Apple bHLH Transcription Factor, Confers Water Stress Resistance by Regulating Stomatal Closure and ROS Homeostasis in Transgenic Tobacco |
| title_full_unstemmed | MdbHLH130, an Apple bHLH Transcription Factor, Confers Water Stress Resistance by Regulating Stomatal Closure and ROS Homeostasis in Transgenic Tobacco |
| title_short | MdbHLH130, an Apple bHLH Transcription Factor, Confers Water Stress Resistance by Regulating Stomatal Closure and ROS Homeostasis in Transgenic Tobacco |
| title_sort | mdbhlh130 an apple bhlh transcription factor confers water stress resistance by regulating stomatal closure and ros homeostasis in transgenic tobacco |
| topic | apple bHLH transcription factor MdbHLH130 water stress stomatal closure ROS-scavenging |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.543696/full |
| work_keys_str_mv | AT qiangzhao mdbhlh130anapplebhlhtranscriptionfactorconferswaterstressresistancebyregulatingstomatalclosureandroshomeostasisintransgenictobacco AT zihaofan mdbhlh130anapplebhlhtranscriptionfactorconferswaterstressresistancebyregulatingstomatalclosureandroshomeostasisintransgenictobacco AT linaqiu mdbhlh130anapplebhlhtranscriptionfactorconferswaterstressresistancebyregulatingstomatalclosureandroshomeostasisintransgenictobacco AT qinqinche mdbhlh130anapplebhlhtranscriptionfactorconferswaterstressresistancebyregulatingstomatalclosureandroshomeostasisintransgenictobacco AT tingwang mdbhlh130anapplebhlhtranscriptionfactorconferswaterstressresistancebyregulatingstomatalclosureandroshomeostasisintransgenictobacco AT yuanyuanli mdbhlh130anapplebhlhtranscriptionfactorconferswaterstressresistancebyregulatingstomatalclosureandroshomeostasisintransgenictobacco AT yongzhangwang mdbhlh130anapplebhlhtranscriptionfactorconferswaterstressresistancebyregulatingstomatalclosureandroshomeostasisintransgenictobacco |