Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis
To compare endogenous cholesterol biosynthesis measured by deuterium incorporation (DI) and mass isotopomer distribution analysis (MIDA), cholesterol fractional and absolute synthetic rates were measured simultaneously by both techniques under identical physiological conditions. Twelve subjects (22...
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| Format: | Article |
| Language: | English |
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Elsevier
2000-09-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520334647 |
| _version_ | 1818671507233046528 |
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| author | Marco Di Buono Peter J.H. Jones Louis Beaumier Linda J. Wykes |
| author_facet | Marco Di Buono Peter J.H. Jones Louis Beaumier Linda J. Wykes |
| author_sort | Marco Di Buono |
| collection | DOAJ |
| description | To compare endogenous cholesterol biosynthesis measured by deuterium incorporation (DI) and mass isotopomer distribution analysis (MIDA), cholesterol fractional and absolute synthetic rates were measured simultaneously by both techniques under identical physiological conditions. Twelve subjects (22 to 39 years of age) underwent a dual stable isotope protocol, involving oral deuterium oxide administration and measurement of incorporation of deuterium into cholesterol coincident with constant infusion of sodium [1-13C]acetate and measurement of the mass isotopomer distribution pattern of newly synthesized cholesterol. Synthesis was determined over 24 h with a 7-h feeding period. Both methods yielded similar measurements of fractional cholesterol synthesis (7.8 ± 2.5% day−1 for DI vs. 6.9 ± 2.2% day−1 for MIDA). Correlation of fractional synthesis across techniques was strong (r = 0.84, P = 0.0007). Absolute synthesis rates were also not different at 24 h (13.4 ± 4.3 mg kg−1 day−1 for DI vs. 11.9 ± 3.6 mg kg−1 day−1 for MIDA, r = 0.79, P < 0.002). We conclude that despite different assumptions and analytical requirements, deuterium incorporation and MIDA yield similar rates of cholesterogenesis in humans when measurements are made over 24 h. The decision as to which method to adopt depends on available clinical and analytical facilities. —Di Buono, M., P. J. H. Jones, L. Beaumier, and L. J. Wykes. Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis. J. Lipid Res. 2000. 41: 1516–1523. |
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| id | doaj.art-c8b667d4edec4184b32debdc5e04be3f |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T07:25:06Z |
| publishDate | 2000-09-01 |
| publisher | Elsevier |
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| series | Journal of Lipid Research |
| spelling | doaj.art-c8b667d4edec4184b32debdc5e04be3f2022-12-21T21:58:40ZengElsevierJournal of Lipid Research0022-22752000-09-0141915161523Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesisMarco Di Buono0Peter J.H. Jones1Louis Beaumier2Linda J. Wykes3School of Dietetics and Human Nutrition, Macdonald Campus of McGill University, Montreal, Québec H9X 3V9, CanadaSchool of Dietetics and Human Nutrition, Macdonald Campus of McGill University, Montreal, Québec H9X 3V9, CanadaSchool of Dietetics and Human Nutrition, Macdonald Campus of McGill University, Montreal, Québec H9X 3V9, Canada; Department of Pediatrics, Montreal Children's Hospital, McGill University, Montreal, Québec H3H 1P3, CanadaSchool of Dietetics and Human Nutrition, Macdonald Campus of McGill University, Montreal, Québec H9X 3V9, Canada; To whom correspondence should be addressed.To compare endogenous cholesterol biosynthesis measured by deuterium incorporation (DI) and mass isotopomer distribution analysis (MIDA), cholesterol fractional and absolute synthetic rates were measured simultaneously by both techniques under identical physiological conditions. Twelve subjects (22 to 39 years of age) underwent a dual stable isotope protocol, involving oral deuterium oxide administration and measurement of incorporation of deuterium into cholesterol coincident with constant infusion of sodium [1-13C]acetate and measurement of the mass isotopomer distribution pattern of newly synthesized cholesterol. Synthesis was determined over 24 h with a 7-h feeding period. Both methods yielded similar measurements of fractional cholesterol synthesis (7.8 ± 2.5% day−1 for DI vs. 6.9 ± 2.2% day−1 for MIDA). Correlation of fractional synthesis across techniques was strong (r = 0.84, P = 0.0007). Absolute synthesis rates were also not different at 24 h (13.4 ± 4.3 mg kg−1 day−1 for DI vs. 11.9 ± 3.6 mg kg−1 day−1 for MIDA, r = 0.79, P < 0.002). We conclude that despite different assumptions and analytical requirements, deuterium incorporation and MIDA yield similar rates of cholesterogenesis in humans when measurements are made over 24 h. The decision as to which method to adopt depends on available clinical and analytical facilities. —Di Buono, M., P. J. H. Jones, L. Beaumier, and L. J. Wykes. Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis. J. Lipid Res. 2000. 41: 1516–1523.http://www.sciencedirect.com/science/article/pii/S0022227520334647deuterium incorporationmass isotopomer distribution analysischolesterol biosynthesisstable isotopesmass spectrometry |
| spellingShingle | Marco Di Buono Peter J.H. Jones Louis Beaumier Linda J. Wykes Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis Journal of Lipid Research deuterium incorporation mass isotopomer distribution analysis cholesterol biosynthesis stable isotopes mass spectrometry |
| title | Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis |
| title_full | Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis |
| title_fullStr | Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis |
| title_full_unstemmed | Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis |
| title_short | Comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis |
| title_sort | comparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis |
| topic | deuterium incorporation mass isotopomer distribution analysis cholesterol biosynthesis stable isotopes mass spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520334647 |
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