Microbial Communities in Retail Draft Beers and the Biofilms They Produce
ABSTRACT In the beer brewing industry, microbial spoilage presents a consistent threat that must be monitored and controlled to ensure the palatability of a finished product. Many of the predominant beer spoilage microbes have been identified and characterized, but the mechanisms of contamination an...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2021-12-01
|
| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/Spectrum.01404-21 |
| _version_ | 1819274645084307456 |
|---|---|
| author | Nikhil Bose Daniel P. Auvil Erica L. Moore Sean D. Moore |
| author_facet | Nikhil Bose Daniel P. Auvil Erica L. Moore Sean D. Moore |
| author_sort | Nikhil Bose |
| collection | DOAJ |
| description | ABSTRACT In the beer brewing industry, microbial spoilage presents a consistent threat that must be monitored and controlled to ensure the palatability of a finished product. Many of the predominant beer spoilage microbes have been identified and characterized, but the mechanisms of contamination and persistence remain an open area of study. Postproduction, many beers are distributed as kegs that are attached to draft delivery systems in retail settings where ample opportunities for microbial spoilage are present. As such, restaurants and bars can experience substantial costs and downtime for cleaning when beer draft lines become heavily contaminated. Spoilage monitoring on the retail side of the beer industry is often overlooked, yet this arena may represent one of the largest threats to the profitability of a beer if its flavor profile becomes substantially distorted by contaminating microbes. In this study, we sampled and cultured microbial communities found in beers dispensed from a retail draft system to identify the contaminating bacteria and yeasts. We also evaluated their capability to establish new biofilms in a controlled setting. Among four tested beer types, we identified over a hundred different contaminant bacteria and nearly 20 wild yeasts. The culturing experiments demonstrated that most of these microbes were viable and capable of joining new biofilm communities. These data provide an important reference for monitoring specific beer spoilage microbes in draft systems and we provide suggestions for cleaning protocol improvements. IMPORTANCE Beer production, packaging, and service are each vulnerable to contamination by microbes that metabolize beer chemicals and impart undesirable flavors, which can result in the disposal of entire batches. Therefore, great effort is taken by brewmasters to reduce and monitor contamination during production and packaging. A commonly overlooked quality control stage of a beer supply chain is at the retail service end, where beer kegs supply draft lines in bars and restaurants under nonsterile conditions. We found that retail draft line contamination is rampant and that routine line cleaning methods are insufficient to efficiently suppress beer spoilage. Thus, many customers unknowingly consume spoiled versions of the beers they consume. This study identified the bacteria and yeast that were resident in retail draft beer samples and also investigated their abilities to colonize tubing material as members of biofilm communities. |
| first_indexed | 2024-12-23T23:11:43Z |
| format | Article |
| id | doaj.art-c8b72923fdc9495789464e64a49c4159 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-12-23T23:11:43Z |
| publishDate | 2021-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-c8b72923fdc9495789464e64a49c41592022-12-21T17:26:39ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972021-12-019310.1128/Spectrum.01404-21Microbial Communities in Retail Draft Beers and the Biofilms They ProduceNikhil Bose0Daniel P. Auvil1Erica L. Moore2Sean D. Moore3Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USABurnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USABurnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USABurnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USAABSTRACT In the beer brewing industry, microbial spoilage presents a consistent threat that must be monitored and controlled to ensure the palatability of a finished product. Many of the predominant beer spoilage microbes have been identified and characterized, but the mechanisms of contamination and persistence remain an open area of study. Postproduction, many beers are distributed as kegs that are attached to draft delivery systems in retail settings where ample opportunities for microbial spoilage are present. As such, restaurants and bars can experience substantial costs and downtime for cleaning when beer draft lines become heavily contaminated. Spoilage monitoring on the retail side of the beer industry is often overlooked, yet this arena may represent one of the largest threats to the profitability of a beer if its flavor profile becomes substantially distorted by contaminating microbes. In this study, we sampled and cultured microbial communities found in beers dispensed from a retail draft system to identify the contaminating bacteria and yeasts. We also evaluated their capability to establish new biofilms in a controlled setting. Among four tested beer types, we identified over a hundred different contaminant bacteria and nearly 20 wild yeasts. The culturing experiments demonstrated that most of these microbes were viable and capable of joining new biofilm communities. These data provide an important reference for monitoring specific beer spoilage microbes in draft systems and we provide suggestions for cleaning protocol improvements. IMPORTANCE Beer production, packaging, and service are each vulnerable to contamination by microbes that metabolize beer chemicals and impart undesirable flavors, which can result in the disposal of entire batches. Therefore, great effort is taken by brewmasters to reduce and monitor contamination during production and packaging. A commonly overlooked quality control stage of a beer supply chain is at the retail service end, where beer kegs supply draft lines in bars and restaurants under nonsterile conditions. We found that retail draft line contamination is rampant and that routine line cleaning methods are insufficient to efficiently suppress beer spoilage. Thus, many customers unknowingly consume spoiled versions of the beers they consume. This study identified the bacteria and yeast that were resident in retail draft beer samples and also investigated their abilities to colonize tubing material as members of biofilm communities.https://journals.asm.org/doi/10.1128/Spectrum.01404-21beerbacteriayeastbiofilmAcetobacterLactobacillus |
| spellingShingle | Nikhil Bose Daniel P. Auvil Erica L. Moore Sean D. Moore Microbial Communities in Retail Draft Beers and the Biofilms They Produce Microbiology Spectrum beer bacteria yeast biofilm Acetobacter Lactobacillus |
| title | Microbial Communities in Retail Draft Beers and the Biofilms They Produce |
| title_full | Microbial Communities in Retail Draft Beers and the Biofilms They Produce |
| title_fullStr | Microbial Communities in Retail Draft Beers and the Biofilms They Produce |
| title_full_unstemmed | Microbial Communities in Retail Draft Beers and the Biofilms They Produce |
| title_short | Microbial Communities in Retail Draft Beers and the Biofilms They Produce |
| title_sort | microbial communities in retail draft beers and the biofilms they produce |
| topic | beer bacteria yeast biofilm Acetobacter Lactobacillus |
| url | https://journals.asm.org/doi/10.1128/Spectrum.01404-21 |
| work_keys_str_mv | AT nikhilbose microbialcommunitiesinretaildraftbeersandthebiofilmstheyproduce AT danielpauvil microbialcommunitiesinretaildraftbeersandthebiofilmstheyproduce AT ericalmoore microbialcommunitiesinretaildraftbeersandthebiofilmstheyproduce AT seandmoore microbialcommunitiesinretaildraftbeersandthebiofilmstheyproduce |