Plasma Extracellular Vesicle Characteristics as Biomarkers of Resectability and Radicality of Surgical Resection in Pancreatic Cancer—A Prospective Cohort Study
Due to possible diagnostic misjudgment of tumor resectability, patients with pancreatic ductal adenocarcinoma (PDAC) might be exposed to non-radical resection or unnecessary laparotomy. With small extracellular vesicles (sEV) obtained by liquid biopsy, we aimed to evaluate their potential as biomark...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/15/3/605 |
| _version_ | 1797625057557610496 |
|---|---|
| author | David Badovinac Katja Goričar Teja Lavrin Hana Zavrtanik Vita Dolžan Metka Lenassi Aleš Tomažič |
| author_facet | David Badovinac Katja Goričar Teja Lavrin Hana Zavrtanik Vita Dolžan Metka Lenassi Aleš Tomažič |
| author_sort | David Badovinac |
| collection | DOAJ |
| description | Due to possible diagnostic misjudgment of tumor resectability, patients with pancreatic ductal adenocarcinoma (PDAC) might be exposed to non-radical resection or unnecessary laparotomy. With small extracellular vesicles (sEV) obtained by liquid biopsy, we aimed to evaluate their potential as biomarkers of tumor resectability, radicality of resection and overall survival (OS). Our prospective study included 83 PDAC patients undergoing surgery with curative intent followed-up longitudinally. sEV were isolated from plasma, and their concentration and size were determined. Fifty patients underwent PDAC resection, and thirty-three had no resection. Preoperatively, patients undergoing resection had higher sEV concentrations than those without resection (<i>p</i> = 0.023). Resection was predicted at the cutoff value of 1.88 × 10<sup>9</sup>/mL for preoperative sEV concentration (<i>p</i> = 0.023) and the cutoff value of 194.8 nm for preoperative mean diameter (<i>p</i> = 0.057). Furthermore, patients with R0 resection demonstrated higher preoperative plasma sEV concentrations than patients with R1/R2 resection (<i>p</i> = 0.014). If sEV concentration was above 1.88 × 10<sup>9</sup>/mL or if the mean diameter was below 194.8 nm, patients had significantly longer OS (<i>p</i> = 0.018 and <i>p</i> = 0.030, respectively). Our proof-of-principle study identified preoperative sEV characteristics as putative biomarkers of feasibility and radicality of PDAC resection that also enable discrimination of patients with worse OS. Liquid biopsy with sEV could aid in PDAC patient stratification and treatment optimization in the future. |
| first_indexed | 2024-03-11T09:51:26Z |
| format | Article |
| id | doaj.art-c8b950de163b446bace4577011743802 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T09:51:26Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-c8b950de163b446bace45770117438022023-11-16T16:14:34ZengMDPI AGCancers2072-66942023-01-0115360510.3390/cancers15030605Plasma Extracellular Vesicle Characteristics as Biomarkers of Resectability and Radicality of Surgical Resection in Pancreatic Cancer—A Prospective Cohort StudyDavid Badovinac0Katja Goričar1Teja Lavrin2Hana Zavrtanik3Vita Dolžan4Metka Lenassi5Aleš Tomažič6Department of Abdominal Surgery, University Medical Centre Ljubljana, Zaloška 7, 1000 Ljubljana, SloveniaInstitute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, SloveniaInstitute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, SloveniaDepartment of Abdominal Surgery, University Medical Centre Ljubljana, Zaloška 7, 1000 Ljubljana, SloveniaInstitute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, SloveniaInstitute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, SloveniaDepartment of Abdominal Surgery, University Medical Centre Ljubljana, Zaloška 7, 1000 Ljubljana, SloveniaDue to possible diagnostic misjudgment of tumor resectability, patients with pancreatic ductal adenocarcinoma (PDAC) might be exposed to non-radical resection or unnecessary laparotomy. With small extracellular vesicles (sEV) obtained by liquid biopsy, we aimed to evaluate their potential as biomarkers of tumor resectability, radicality of resection and overall survival (OS). Our prospective study included 83 PDAC patients undergoing surgery with curative intent followed-up longitudinally. sEV were isolated from plasma, and their concentration and size were determined. Fifty patients underwent PDAC resection, and thirty-three had no resection. Preoperatively, patients undergoing resection had higher sEV concentrations than those without resection (<i>p</i> = 0.023). Resection was predicted at the cutoff value of 1.88 × 10<sup>9</sup>/mL for preoperative sEV concentration (<i>p</i> = 0.023) and the cutoff value of 194.8 nm for preoperative mean diameter (<i>p</i> = 0.057). Furthermore, patients with R0 resection demonstrated higher preoperative plasma sEV concentrations than patients with R1/R2 resection (<i>p</i> = 0.014). If sEV concentration was above 1.88 × 10<sup>9</sup>/mL or if the mean diameter was below 194.8 nm, patients had significantly longer OS (<i>p</i> = 0.018 and <i>p</i> = 0.030, respectively). Our proof-of-principle study identified preoperative sEV characteristics as putative biomarkers of feasibility and radicality of PDAC resection that also enable discrimination of patients with worse OS. Liquid biopsy with sEV could aid in PDAC patient stratification and treatment optimization in the future.https://www.mdpi.com/2072-6694/15/3/605liquid biopsybiomarkersextracellular vesiclespancreatic ductal adenocarcinomaresectionpatient stratification |
| spellingShingle | David Badovinac Katja Goričar Teja Lavrin Hana Zavrtanik Vita Dolžan Metka Lenassi Aleš Tomažič Plasma Extracellular Vesicle Characteristics as Biomarkers of Resectability and Radicality of Surgical Resection in Pancreatic Cancer—A Prospective Cohort Study Cancers liquid biopsy biomarkers extracellular vesicles pancreatic ductal adenocarcinoma resection patient stratification |
| title | Plasma Extracellular Vesicle Characteristics as Biomarkers of Resectability and Radicality of Surgical Resection in Pancreatic Cancer—A Prospective Cohort Study |
| title_full | Plasma Extracellular Vesicle Characteristics as Biomarkers of Resectability and Radicality of Surgical Resection in Pancreatic Cancer—A Prospective Cohort Study |
| title_fullStr | Plasma Extracellular Vesicle Characteristics as Biomarkers of Resectability and Radicality of Surgical Resection in Pancreatic Cancer—A Prospective Cohort Study |
| title_full_unstemmed | Plasma Extracellular Vesicle Characteristics as Biomarkers of Resectability and Radicality of Surgical Resection in Pancreatic Cancer—A Prospective Cohort Study |
| title_short | Plasma Extracellular Vesicle Characteristics as Biomarkers of Resectability and Radicality of Surgical Resection in Pancreatic Cancer—A Prospective Cohort Study |
| title_sort | plasma extracellular vesicle characteristics as biomarkers of resectability and radicality of surgical resection in pancreatic cancer a prospective cohort study |
| topic | liquid biopsy biomarkers extracellular vesicles pancreatic ductal adenocarcinoma resection patient stratification |
| url | https://www.mdpi.com/2072-6694/15/3/605 |
| work_keys_str_mv | AT davidbadovinac plasmaextracellularvesiclecharacteristicsasbiomarkersofresectabilityandradicalityofsurgicalresectioninpancreaticcanceraprospectivecohortstudy AT katjagoricar plasmaextracellularvesiclecharacteristicsasbiomarkersofresectabilityandradicalityofsurgicalresectioninpancreaticcanceraprospectivecohortstudy AT tejalavrin plasmaextracellularvesiclecharacteristicsasbiomarkersofresectabilityandradicalityofsurgicalresectioninpancreaticcanceraprospectivecohortstudy AT hanazavrtanik plasmaextracellularvesiclecharacteristicsasbiomarkersofresectabilityandradicalityofsurgicalresectioninpancreaticcanceraprospectivecohortstudy AT vitadolzan plasmaextracellularvesiclecharacteristicsasbiomarkersofresectabilityandradicalityofsurgicalresectioninpancreaticcanceraprospectivecohortstudy AT metkalenassi plasmaextracellularvesiclecharacteristicsasbiomarkersofresectabilityandradicalityofsurgicalresectioninpancreaticcanceraprospectivecohortstudy AT alestomazic plasmaextracellularvesiclecharacteristicsasbiomarkersofresectabilityandradicalityofsurgicalresectioninpancreaticcanceraprospectivecohortstudy |