Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23
The mechanisms contributing to persistence of coagulase-negative staphylococci are diverse; to better understanding of their dynamics, the characterization of nosocomial isolates is needed. Our aim was to characterize phenotypic and molecular characteristics of Staphylococcus epidermidis and Staphyl...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-09-01
|
| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867016301015 |
| _version_ | 1818838046731141120 |
|---|---|
| author | Adrián Martínez-Meléndez Rayo Morfín-Otero Licet Villarreal-Treviño Adrián Camacho-Ortíz Gloria González-González Jorge Llaca-Díaz Eduardo Rodríguez-Noriega Elvira Garza-González |
| author_facet | Adrián Martínez-Meléndez Rayo Morfín-Otero Licet Villarreal-Treviño Adrián Camacho-Ortíz Gloria González-González Jorge Llaca-Díaz Eduardo Rodríguez-Noriega Elvira Garza-González |
| author_sort | Adrián Martínez-Meléndez |
| collection | DOAJ |
| description | The mechanisms contributing to persistence of coagulase-negative staphylococci are diverse; to better understanding of their dynamics, the characterization of nosocomial isolates is needed. Our aim was to characterize phenotypic and molecular characteristics of Staphylococcus epidermidis and Staphylococcus haemolyticus human blood isolates from two tertiary care hospitals in Mexico, the Hospital Universitario in Monterrey and the Hospital Civil in Guadalajara.Antimicrobial susceptibility was determined. Biofilm formation was assessed by crystal violet staining. Detection of the ica operon and Staphylococcal Cassette Chromosome mec typing were performed by PCR. Clonal relatedness was determined by Pulsed-fiel gel electrophoresis and Multi locus sequence typing.Methicillin-resistance was 85.5% and 93.2% for S. epidermidis and S. haemolyticus, respectively. Both species showed resistance >70% to norfloxacin, clindamycin, levofloxacin, trimethoprim/sulfamethoxazole, and erythromycin. Three S. epidermidis and two S. haemolyticus isolates were linezolid-resistant (one isolate of each species was cfr+). Most isolates of both species were strong biofilm producers (92.8% of S. epidermidis and 72.9% of S. haemolyticus). The ica operon was amplified in 36 (43.4%) S. epidermidis isolates. SCCmec type IV was found in 47.2% of the S. epidermidis isolates and SCCmec type V in 14.5% of S. haemolyticus isolates. No clonal relatedness was found in either species. Resistance to clindamycin, levofloxacin, erythromycin, oxacillin, and cefoxitin was associated with biofilm production for both species (p < 0.05). A G2576T mutation in 23S rRNA gene was detected in an S. haemolyticus linezolid-resistant isolate. All linezolid-resistant S. epidermidis isolates belonged to ST23; isolate with SCCmec type IV belonged to ST7, and isolate with SCCmec type III belonged to ST2. This is the first report of ST7 in Mexico.There was a high genetic diversity in both species, though both species shared characteristics that may contibute to virulence. Keywords: Linezolid resistance, Staphylococcal Cassette Chromosome mec, Coagulase-negative staphylococci, Multilocus Sequence Typing |
| first_indexed | 2024-12-19T03:32:10Z |
| format | Article |
| id | doaj.art-c8b9cab328254e2db3a8ff5be2ca64cc |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-19T03:32:10Z |
| publishDate | 2016-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-c8b9cab328254e2db3a8ff5be2ca64cc2022-12-21T20:37:28ZengElsevierBrazilian Journal of Infectious Diseases1413-86702016-09-01205419428S1413-86702016000500419Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23Adrián Martínez-Meléndez0Rayo Morfín-Otero1Licet Villarreal-Treviño2Adrián Camacho-Ortíz3Gloria González-González4Jorge Llaca-Díaz5Eduardo Rodríguez-Noriega6Elvira Garza-González7Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, Monterrey, MexicoHospital Civil de Guadalajara “Fray Antonio Alcalde”, Guadalajara, Mexico; Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Instituto de Patología Infecciosa y Experimental, Guadalajara, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, Monterrey, MexicoUniversidad Autónoma de Nuevo León, Hospital Universitario Dr. José Eleuterio González, Servicio de Infectología, Monterrey, MexicoUniversidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Microbiología, México, Monterrey, MexicoUniversidad Autónoma de Nuevo León, Hospital Universitario Dr. José Eleuterio González, Departamento de Patología Clínica, Monterrey, MexicoHospital Civil de Guadalajara “Fray Antonio Alcalde”, Guadalajara, MexicoUniversidad Autónoma de Nuevo León, Hospital Universitario Dr. José Eleuterio González, Departamento de Patología Clínica, Monterrey, Mexico; Universidad Autónoma de Nuevo León, Hospital Universitario Dr. José Eleuterio González, Servicio de Gastroenterología, Monterrey, Mexico; Corresponding author.The mechanisms contributing to persistence of coagulase-negative staphylococci are diverse; to better understanding of their dynamics, the characterization of nosocomial isolates is needed. Our aim was to characterize phenotypic and molecular characteristics of Staphylococcus epidermidis and Staphylococcus haemolyticus human blood isolates from two tertiary care hospitals in Mexico, the Hospital Universitario in Monterrey and the Hospital Civil in Guadalajara.Antimicrobial susceptibility was determined. Biofilm formation was assessed by crystal violet staining. Detection of the ica operon and Staphylococcal Cassette Chromosome mec typing were performed by PCR. Clonal relatedness was determined by Pulsed-fiel gel electrophoresis and Multi locus sequence typing.Methicillin-resistance was 85.5% and 93.2% for S. epidermidis and S. haemolyticus, respectively. Both species showed resistance >70% to norfloxacin, clindamycin, levofloxacin, trimethoprim/sulfamethoxazole, and erythromycin. Three S. epidermidis and two S. haemolyticus isolates were linezolid-resistant (one isolate of each species was cfr+). Most isolates of both species were strong biofilm producers (92.8% of S. epidermidis and 72.9% of S. haemolyticus). The ica operon was amplified in 36 (43.4%) S. epidermidis isolates. SCCmec type IV was found in 47.2% of the S. epidermidis isolates and SCCmec type V in 14.5% of S. haemolyticus isolates. No clonal relatedness was found in either species. Resistance to clindamycin, levofloxacin, erythromycin, oxacillin, and cefoxitin was associated with biofilm production for both species (p < 0.05). A G2576T mutation in 23S rRNA gene was detected in an S. haemolyticus linezolid-resistant isolate. All linezolid-resistant S. epidermidis isolates belonged to ST23; isolate with SCCmec type IV belonged to ST7, and isolate with SCCmec type III belonged to ST2. This is the first report of ST7 in Mexico.There was a high genetic diversity in both species, though both species shared characteristics that may contibute to virulence. Keywords: Linezolid resistance, Staphylococcal Cassette Chromosome mec, Coagulase-negative staphylococci, Multilocus Sequence Typinghttp://www.sciencedirect.com/science/article/pii/S1413867016301015 |
| spellingShingle | Adrián Martínez-Meléndez Rayo Morfín-Otero Licet Villarreal-Treviño Adrián Camacho-Ortíz Gloria González-González Jorge Llaca-Díaz Eduardo Rodríguez-Noriega Elvira Garza-González Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23 Brazilian Journal of Infectious Diseases |
| title | Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23 |
| title_full | Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23 |
| title_fullStr | Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23 |
| title_full_unstemmed | Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23 |
| title_short | Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23 |
| title_sort | molecular epidemiology of coagulase negative bloodstream isolates detection of staphylococcus epidermidis st2 st7 and linezolid resistant st23 |
| url | http://www.sciencedirect.com/science/article/pii/S1413867016301015 |
| work_keys_str_mv | AT adrianmartinezmelendez molecularepidemiologyofcoagulasenegativebloodstreamisolatesdetectionofstaphylococcusepidermidisst2st7andlinezolidresistantst23 AT rayomorfinotero molecularepidemiologyofcoagulasenegativebloodstreamisolatesdetectionofstaphylococcusepidermidisst2st7andlinezolidresistantst23 AT licetvillarrealtrevino molecularepidemiologyofcoagulasenegativebloodstreamisolatesdetectionofstaphylococcusepidermidisst2st7andlinezolidresistantst23 AT adriancamachoortiz molecularepidemiologyofcoagulasenegativebloodstreamisolatesdetectionofstaphylococcusepidermidisst2st7andlinezolidresistantst23 AT gloriagonzalezgonzalez molecularepidemiologyofcoagulasenegativebloodstreamisolatesdetectionofstaphylococcusepidermidisst2st7andlinezolidresistantst23 AT jorgellacadiaz molecularepidemiologyofcoagulasenegativebloodstreamisolatesdetectionofstaphylococcusepidermidisst2st7andlinezolidresistantst23 AT eduardorodrigueznoriega molecularepidemiologyofcoagulasenegativebloodstreamisolatesdetectionofstaphylococcusepidermidisst2st7andlinezolidresistantst23 AT elviragarzagonzalez molecularepidemiologyofcoagulasenegativebloodstreamisolatesdetectionofstaphylococcusepidermidisst2st7andlinezolidresistantst23 |