Enrichment Pretreatment Expands the Microbial Diversity Cultivated from Marine Sediments
The majority of the microbial diversity in nature has not been recovered through cultivation. Enrichment is a classical technique widely used in the selective cultivation of specific taxa. Whether enrichment is suitable for cultivation studies that aim to recover large numbers of species remains lit...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/11/2771 |
| _version_ | 1797458332818079744 |
|---|---|
| author | Meng Wang Ning Zheng Xuan Li Kun Zhao Bin-Bin Xie |
| author_facet | Meng Wang Ning Zheng Xuan Li Kun Zhao Bin-Bin Xie |
| author_sort | Meng Wang |
| collection | DOAJ |
| description | The majority of the microbial diversity in nature has not been recovered through cultivation. Enrichment is a classical technique widely used in the selective cultivation of specific taxa. Whether enrichment is suitable for cultivation studies that aim to recover large numbers of species remains little explored. To address this issue, we evaluated the potential of enrichment pretreatment in the cultivation of bacteria from marine sediments. Upon obtaining and classifying a total of 943 pure cultures from chitin and cellulose enrichment pretreatment systems and a control system, our results showed that species obtained using enrichment pretreatment differed greatly from those without enrichment. Multiple enrichment media and different enrichment times increased the number of cultivated species in a sample. Amplicon sequencing showed that the increased relative abundance during pretreatment contributed greatly to bacterial cultivation. The testing of degradation abilities against chitin and cellulose and the whole-genome sequencing of representative strains suggested that microorganism–microorganism interactions play roles in the expanded diversity of cultivated bacteria. This study provides new insights into the abilities of enrichment in exploring cultivable diversity and mining microbial resources. |
| first_indexed | 2024-03-09T16:35:36Z |
| format | Article |
| id | doaj.art-c8bb54f3da4b4d6eb3c74ec687d1ab22 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:35:36Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c8bb54f3da4b4d6eb3c74ec687d1ab222023-11-24T14:57:12ZengMDPI AGMicroorganisms2076-26072023-11-011111277110.3390/microorganisms11112771Enrichment Pretreatment Expands the Microbial Diversity Cultivated from Marine SedimentsMeng Wang0Ning Zheng1Xuan Li2Kun Zhao3Bin-Bin Xie4State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, ChinaThe majority of the microbial diversity in nature has not been recovered through cultivation. Enrichment is a classical technique widely used in the selective cultivation of specific taxa. Whether enrichment is suitable for cultivation studies that aim to recover large numbers of species remains little explored. To address this issue, we evaluated the potential of enrichment pretreatment in the cultivation of bacteria from marine sediments. Upon obtaining and classifying a total of 943 pure cultures from chitin and cellulose enrichment pretreatment systems and a control system, our results showed that species obtained using enrichment pretreatment differed greatly from those without enrichment. Multiple enrichment media and different enrichment times increased the number of cultivated species in a sample. Amplicon sequencing showed that the increased relative abundance during pretreatment contributed greatly to bacterial cultivation. The testing of degradation abilities against chitin and cellulose and the whole-genome sequencing of representative strains suggested that microorganism–microorganism interactions play roles in the expanded diversity of cultivated bacteria. This study provides new insights into the abilities of enrichment in exploring cultivable diversity and mining microbial resources.https://www.mdpi.com/2076-2607/11/11/2771enrichment pretreatmentcultivationchitincellulosenovel species |
| spellingShingle | Meng Wang Ning Zheng Xuan Li Kun Zhao Bin-Bin Xie Enrichment Pretreatment Expands the Microbial Diversity Cultivated from Marine Sediments Microorganisms enrichment pretreatment cultivation chitin cellulose novel species |
| title | Enrichment Pretreatment Expands the Microbial Diversity Cultivated from Marine Sediments |
| title_full | Enrichment Pretreatment Expands the Microbial Diversity Cultivated from Marine Sediments |
| title_fullStr | Enrichment Pretreatment Expands the Microbial Diversity Cultivated from Marine Sediments |
| title_full_unstemmed | Enrichment Pretreatment Expands the Microbial Diversity Cultivated from Marine Sediments |
| title_short | Enrichment Pretreatment Expands the Microbial Diversity Cultivated from Marine Sediments |
| title_sort | enrichment pretreatment expands the microbial diversity cultivated from marine sediments |
| topic | enrichment pretreatment cultivation chitin cellulose novel species |
| url | https://www.mdpi.com/2076-2607/11/11/2771 |
| work_keys_str_mv | AT mengwang enrichmentpretreatmentexpandsthemicrobialdiversitycultivatedfrommarinesediments AT ningzheng enrichmentpretreatmentexpandsthemicrobialdiversitycultivatedfrommarinesediments AT xuanli enrichmentpretreatmentexpandsthemicrobialdiversitycultivatedfrommarinesediments AT kunzhao enrichmentpretreatmentexpandsthemicrobialdiversitycultivatedfrommarinesediments AT binbinxie enrichmentpretreatmentexpandsthemicrobialdiversitycultivatedfrommarinesediments |