Hs27 fibroblast response to contact guidance cues
Abstract Contact guidance is the phenomena of how cells respond to the topography of their external environment. The morphological and dynamic cell responses are strongly influenced by topographic features such as lateral and vertical dimensions, namely, ridge and groove widths and groove depth ( $$...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-48913-9 |
| _version_ | 1797398102496247808 |
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| author | C. Kim M. Robitaille J. Christodoulides Y. Ng M. Raphael W. Kang |
| author_facet | C. Kim M. Robitaille J. Christodoulides Y. Ng M. Raphael W. Kang |
| author_sort | C. Kim |
| collection | DOAJ |
| description | Abstract Contact guidance is the phenomena of how cells respond to the topography of their external environment. The morphological and dynamic cell responses are strongly influenced by topographic features such as lateral and vertical dimensions, namely, ridge and groove widths and groove depth ( $${{\text{R}}}_{{\text{w}}}, {{\text{G}}}_{{\text{w}}},\,\mathrm{and}\,\,{{\text{G}}}_{{\text{D}}}$$ R w , G w , and G D , respectively). However, experimental studies that independently quantify the effect of the individual dimensions as well as their coupling on cellular function are still limited. In this work, we perform extensive parametric studies in the dimensional space—well beyond the previously studied range in the literature—to explore topographical effects on morphology and migration of Hs27 fibroblasts via static and dynamic analyses of live cell images. Our static analysis reveals that the $${{\text{G}}}_{{\text{D}}}$$ G D is most significant, followed by the $${{\text{R}}}_{{\text{w}}}$$ R w . The fibroblasts appear to be more elongated and aligned in the groove direction as the $${{\text{G}}}_{{\text{D}}}$$ G D increases, but their trend changes after 725 nm. Interestingly, the cell shape and alignment show a very strong correlation regardless of $${{\text{G}}}_{{\text{D}}}$$ G D . Our dynamic analysis confirms that directional cell migration is also strongly influenced by the $${{\text{G}}}_{{\text{D}}}$$ G D , while the effect of the $${{\text{R}}}_{{\text{w}}}$$ R w and $${{\text{G}}}_{{\text{w}}}$$ G w is statistically insignificant. Directional cell migration, as observed in the static cell behavior, shows the statistically significant transition when the $${{\text{G}}}_{{\text{D}}}$$ G D is 725 nm, showing the intimate links between cell morphology and migration. We propose possible scenarios to offer mechanistic explanations of the observed cell behavior. |
| first_indexed | 2024-03-09T01:19:54Z |
| format | Article |
| id | doaj.art-c8bf378eccda4c589a8ff5cc818fb2f3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T01:19:54Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c8bf378eccda4c589a8ff5cc818fb2f32023-12-10T12:13:51ZengNature PortfolioScientific Reports2045-23222023-12-0113111310.1038/s41598-023-48913-9Hs27 fibroblast response to contact guidance cuesC. Kim0M. Robitaille1J. Christodoulides2Y. Ng3M. Raphael4W. Kang5Mechanical Engineering, School for Engineering of Matter, Transport and Energy, Arizona State UniversityUS Naval Research LaboratoryUS Naval Research LaboratoryMechanical Engineering, School for Engineering of Matter, Transport and Energy, Arizona State UniversityUS Naval Research LaboratoryMechanical Engineering, School for Engineering of Matter, Transport and Energy, Arizona State UniversityAbstract Contact guidance is the phenomena of how cells respond to the topography of their external environment. The morphological and dynamic cell responses are strongly influenced by topographic features such as lateral and vertical dimensions, namely, ridge and groove widths and groove depth ( $${{\text{R}}}_{{\text{w}}}, {{\text{G}}}_{{\text{w}}},\,\mathrm{and}\,\,{{\text{G}}}_{{\text{D}}}$$ R w , G w , and G D , respectively). However, experimental studies that independently quantify the effect of the individual dimensions as well as their coupling on cellular function are still limited. In this work, we perform extensive parametric studies in the dimensional space—well beyond the previously studied range in the literature—to explore topographical effects on morphology and migration of Hs27 fibroblasts via static and dynamic analyses of live cell images. Our static analysis reveals that the $${{\text{G}}}_{{\text{D}}}$$ G D is most significant, followed by the $${{\text{R}}}_{{\text{w}}}$$ R w . The fibroblasts appear to be more elongated and aligned in the groove direction as the $${{\text{G}}}_{{\text{D}}}$$ G D increases, but their trend changes after 725 nm. Interestingly, the cell shape and alignment show a very strong correlation regardless of $${{\text{G}}}_{{\text{D}}}$$ G D . Our dynamic analysis confirms that directional cell migration is also strongly influenced by the $${{\text{G}}}_{{\text{D}}}$$ G D , while the effect of the $${{\text{R}}}_{{\text{w}}}$$ R w and $${{\text{G}}}_{{\text{w}}}$$ G w is statistically insignificant. Directional cell migration, as observed in the static cell behavior, shows the statistically significant transition when the $${{\text{G}}}_{{\text{D}}}$$ G D is 725 nm, showing the intimate links between cell morphology and migration. We propose possible scenarios to offer mechanistic explanations of the observed cell behavior.https://doi.org/10.1038/s41598-023-48913-9 |
| spellingShingle | C. Kim M. Robitaille J. Christodoulides Y. Ng M. Raphael W. Kang Hs27 fibroblast response to contact guidance cues Scientific Reports |
| title | Hs27 fibroblast response to contact guidance cues |
| title_full | Hs27 fibroblast response to contact guidance cues |
| title_fullStr | Hs27 fibroblast response to contact guidance cues |
| title_full_unstemmed | Hs27 fibroblast response to contact guidance cues |
| title_short | Hs27 fibroblast response to contact guidance cues |
| title_sort | hs27 fibroblast response to contact guidance cues |
| url | https://doi.org/10.1038/s41598-023-48913-9 |
| work_keys_str_mv | AT ckim hs27fibroblastresponsetocontactguidancecues AT mrobitaille hs27fibroblastresponsetocontactguidancecues AT jchristodoulides hs27fibroblastresponsetocontactguidancecues AT yng hs27fibroblastresponsetocontactguidancecues AT mraphael hs27fibroblastresponsetocontactguidancecues AT wkang hs27fibroblastresponsetocontactguidancecues |