Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection
Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) infection, currently lacks specific preventive and therapeutic interventions. Here, we demonstrated that Pien Tze Huang (PZH) could dose-dependently inhibit EV-A71 replication at the cellular level, resulting in significant redu...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1251731/full |
| _version_ | 1797648891496103936 |
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| author | Huiqiang Wang Fenbei Chen Shicong Wang Yuhuan Li Ting Liu Yinghong Li Hongbin Deng Jingwen Dong Jing Pang Danqing Song Dousheng Zhang Juan Yu Yanxiang Wang |
| author_facet | Huiqiang Wang Fenbei Chen Shicong Wang Yuhuan Li Ting Liu Yinghong Li Hongbin Deng Jingwen Dong Jing Pang Danqing Song Dousheng Zhang Juan Yu Yanxiang Wang |
| author_sort | Huiqiang Wang |
| collection | DOAJ |
| description | Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) infection, currently lacks specific preventive and therapeutic interventions. Here, we demonstrated that Pien Tze Huang (PZH) could dose-dependently inhibit EV-A71 replication at the cellular level, resulting in significant reductions in EV-A71 virus protein 1 (VP1) expression and viral yields in Vero and human rhabdomyosarcoma cells. More importantly, we confirmed that PZH could protect mice from EV-A71 infection for the first time, with Ribavirin serving as a positive control. PZH treatment reduced EV-A71 VP1 protein expression, viral yields in infected muscles, and improved muscle pathology. Additionally, we conducted a preliminary mechanism study using quantitative proteomics. The results suggested that the suppression of the PI3K/AKT/mTOR and NF-κB signaling pathways may contribute to the anti-EV-A71 activity of PZH. These findings provide strong evidence supporting the potential therapeutic application of PZH for EV-A71 infection management. |
| first_indexed | 2024-03-11T15:38:31Z |
| format | Article |
| id | doaj.art-c8c09e9a8cf54250acb8daeee8c15836 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-11T15:38:31Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-c8c09e9a8cf54250acb8daeee8c158362023-10-26T13:15:18ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-10-011410.3389/fphar.2023.12517311251731Evaluation and mechanism study of Pien Tze Huang against EV-A71 infectionHuiqiang Wang0Fenbei Chen1Shicong Wang2Yuhuan Li3Ting Liu4Yinghong Li5Hongbin Deng6Jingwen Dong7Jing Pang8Danqing Song9Dousheng Zhang10Juan Yu11Yanxiang Wang12Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaFujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development, Zhangzhou, ChinaBeijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaInstitute for Drug Control, National Institute for Food and Drug Control, Beijing, ChinaBeijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaInstitute for Drug Control, National Institute for Food and Drug Control, Beijing, ChinaFujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development, Zhangzhou, ChinaBeijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaHand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) infection, currently lacks specific preventive and therapeutic interventions. Here, we demonstrated that Pien Tze Huang (PZH) could dose-dependently inhibit EV-A71 replication at the cellular level, resulting in significant reductions in EV-A71 virus protein 1 (VP1) expression and viral yields in Vero and human rhabdomyosarcoma cells. More importantly, we confirmed that PZH could protect mice from EV-A71 infection for the first time, with Ribavirin serving as a positive control. PZH treatment reduced EV-A71 VP1 protein expression, viral yields in infected muscles, and improved muscle pathology. Additionally, we conducted a preliminary mechanism study using quantitative proteomics. The results suggested that the suppression of the PI3K/AKT/mTOR and NF-κB signaling pathways may contribute to the anti-EV-A71 activity of PZH. These findings provide strong evidence supporting the potential therapeutic application of PZH for EV-A71 infection management.https://www.frontiersin.org/articles/10.3389/fphar.2023.1251731/fullenterovirus A71Pien Tze Huangmulticomponent drughostcytokineproteomics |
| spellingShingle | Huiqiang Wang Fenbei Chen Shicong Wang Yuhuan Li Ting Liu Yinghong Li Hongbin Deng Jingwen Dong Jing Pang Danqing Song Dousheng Zhang Juan Yu Yanxiang Wang Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection Frontiers in Pharmacology enterovirus A71 Pien Tze Huang multicomponent drug host cytokine proteomics |
| title | Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection |
| title_full | Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection |
| title_fullStr | Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection |
| title_full_unstemmed | Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection |
| title_short | Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection |
| title_sort | evaluation and mechanism study of pien tze huang against ev a71 infection |
| topic | enterovirus A71 Pien Tze Huang multicomponent drug host cytokine proteomics |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1251731/full |
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