Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections
Three human protoparvoviruses, bufavirus (BuV), tusavirus (TuV) and cutavirus (CuV), have recently been discovered in diarrheal stool. BuV has been associated with diarrhea and CuV with cutaneous T-cell lymphoma, but there are hardly any data for TuV or CuV in stool or respiratory samples. Hence, us...
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| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/3/483 |
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| author | Ushanandini Mohanraj Maija Jokinen Rajita Rayamajhi Thapa Minna Paloniemi Timo Vesikari Maija Lappalainen Eveliina Tarkka Zaiga Nora-Krūkle Anda Vilmane Kim Vettenranta Charles Mangani Sami Oikarinen Yue-Mei Fan Per Ashorn Elina Väisänen Maria Söderlund-Venermo |
| author_facet | Ushanandini Mohanraj Maija Jokinen Rajita Rayamajhi Thapa Minna Paloniemi Timo Vesikari Maija Lappalainen Eveliina Tarkka Zaiga Nora-Krūkle Anda Vilmane Kim Vettenranta Charles Mangani Sami Oikarinen Yue-Mei Fan Per Ashorn Elina Väisänen Maria Söderlund-Venermo |
| author_sort | Ushanandini Mohanraj |
| collection | DOAJ |
| description | Three human protoparvoviruses, bufavirus (BuV), tusavirus (TuV) and cutavirus (CuV), have recently been discovered in diarrheal stool. BuV has been associated with diarrhea and CuV with cutaneous T-cell lymphoma, but there are hardly any data for TuV or CuV in stool or respiratory samples. Hence, using qPCR and IgG enzyme immunoassays, we analyzed 1072 stool, 316 respiratory and 445 serum or plasma samples from 1098 patients with and without gastroenteritis (GE) or respiratory-tract infections (RTI) from Finland, Latvia and Malawi. The overall CuV-DNA prevalences in stool samples ranged between 0–6.1% among our six patient cohorts. In Finland, CuV DNA was significantly more prevalent in GE patients above rather than below 60 years of age (5.1% vs 0.2%). CuV DNA was more prevalent in stools among Latvian and Malawian children compared with Finnish children. In 10/11 CuV DNA-positive adults and 4/6 CuV DNA-positive children with GE, no known causal pathogens were detected. Interestingly, for the first time, CuV DNA was observed in two nasopharyngeal aspirates from children with RTI and the rare TuV in diarrheal stools of two adults. Our results provide new insights on the occurrence of human protoparvoviruses in GE and RTI in different countries. |
| first_indexed | 2024-03-10T13:13:51Z |
| format | Article |
| id | doaj.art-c8c10e7c2263454da169a87588e3305f |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T13:13:51Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c8c10e7c2263454da169a87588e3305f2023-11-21T10:35:25ZengMDPI AGViruses1999-49152021-03-0113348310.3390/v13030483Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal InfectionsUshanandini Mohanraj0Maija Jokinen1Rajita Rayamajhi Thapa2Minna Paloniemi3Timo Vesikari4Maija Lappalainen5Eveliina Tarkka6Zaiga Nora-Krūkle7Anda Vilmane8Kim Vettenranta9Charles Mangani10Sami Oikarinen11Yue-Mei Fan12Per Ashorn13Elina Väisänen14Maria Söderlund-Venermo15Department of Virology, University of Helsinki, 00290 Helsinki, FinlandDepartment of Virology, University of Helsinki, 00290 Helsinki, FinlandDepartment of Virology, University of Helsinki, 00290 Helsinki, FinlandFaculty of Medicine and Health Technology, Tampere University, 33100 Tampere, FinlandNordic Research Network Oy, 33700 Tampere, FinlandHelsinki University Hospital Laboratory (HUSLAB), 00290 Helsinki, FinlandHelsinki University Hospital Laboratory (HUSLAB), 00290 Helsinki, FinlandInstitute of Microbiology and Virology, Rīga Stradiņš University, 1067 Riga, LatviaInstitute of Microbiology and Virology, Rīga Stradiņš University, 1067 Riga, LatviaHelsinki University Hospital, 00280 Helsinki, FinlandCollege of Medicine, University of Malawi, Blantyre 3, MalawiFaculty of Medicine and Health Technology, Tampere University, 33100 Tampere, FinlandFaculty of Medicine and Health Technology, Tampere University, 33100 Tampere, FinlandFaculty of Medicine and Health Technology, Tampere University, 33100 Tampere, FinlandDepartment of Virology, University of Helsinki, 00290 Helsinki, FinlandDepartment of Virology, University of Helsinki, 00290 Helsinki, FinlandThree human protoparvoviruses, bufavirus (BuV), tusavirus (TuV) and cutavirus (CuV), have recently been discovered in diarrheal stool. BuV has been associated with diarrhea and CuV with cutaneous T-cell lymphoma, but there are hardly any data for TuV or CuV in stool or respiratory samples. Hence, using qPCR and IgG enzyme immunoassays, we analyzed 1072 stool, 316 respiratory and 445 serum or plasma samples from 1098 patients with and without gastroenteritis (GE) or respiratory-tract infections (RTI) from Finland, Latvia and Malawi. The overall CuV-DNA prevalences in stool samples ranged between 0–6.1% among our six patient cohorts. In Finland, CuV DNA was significantly more prevalent in GE patients above rather than below 60 years of age (5.1% vs 0.2%). CuV DNA was more prevalent in stools among Latvian and Malawian children compared with Finnish children. In 10/11 CuV DNA-positive adults and 4/6 CuV DNA-positive children with GE, no known causal pathogens were detected. Interestingly, for the first time, CuV DNA was observed in two nasopharyngeal aspirates from children with RTI and the rare TuV in diarrheal stools of two adults. Our results provide new insights on the occurrence of human protoparvoviruses in GE and RTI in different countries.https://www.mdpi.com/1999-4915/13/3/483parvovirusbufavirustusaviruscutavirusgastroenteritisrespiratory-tract infection |
| spellingShingle | Ushanandini Mohanraj Maija Jokinen Rajita Rayamajhi Thapa Minna Paloniemi Timo Vesikari Maija Lappalainen Eveliina Tarkka Zaiga Nora-Krūkle Anda Vilmane Kim Vettenranta Charles Mangani Sami Oikarinen Yue-Mei Fan Per Ashorn Elina Väisänen Maria Söderlund-Venermo Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections Viruses parvovirus bufavirus tusavirus cutavirus gastroenteritis respiratory-tract infection |
| title | Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections |
| title_full | Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections |
| title_fullStr | Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections |
| title_full_unstemmed | Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections |
| title_short | Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections |
| title_sort | human protoparvovirus dna and igg in children and adults with and without respiratory or gastrointestinal infections |
| topic | parvovirus bufavirus tusavirus cutavirus gastroenteritis respiratory-tract infection |
| url | https://www.mdpi.com/1999-4915/13/3/483 |
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