Evaluation and reconditioning of donor organs for transplantation through ex vivo lung perfusion
ABSTRACT Objective: To assess the feasibility and impact of ex vivo lung perfusion with hyperoncotic solution (Steen Solution™) in the utilization of these organs in Brazil. Methods: In this prospective study, we subjected five lungs considered to be high risk for transplantation to 4 hours of ex...
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| Format: | Article |
| Language: | English |
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
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| Series: | Einstein (São Paulo) |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082019000400200&lng=en&tlng=en |
| _version_ | 1818128837978882048 |
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| author | Luis Gustavo Abdalla Karina Andrighetti de Oliveira-Braga Lucas Matos Fernandes Marcos Naoyuki Samano Paula Refinetti Camerini Paulo Manuel Pêgo-Fernandes |
| author_facet | Luis Gustavo Abdalla Karina Andrighetti de Oliveira-Braga Lucas Matos Fernandes Marcos Naoyuki Samano Paula Refinetti Camerini Paulo Manuel Pêgo-Fernandes |
| author_sort | Luis Gustavo Abdalla |
| collection | DOAJ |
| description | ABSTRACT Objective: To assess the feasibility and impact of ex vivo lung perfusion with hyperoncotic solution (Steen Solution™) in the utilization of these organs in Brazil. Methods: In this prospective study, we subjected five lungs considered to be high risk for transplantation to 4 hours of ex vivo lung perfusion, with evaluation of oxygenation capacity. High-risk donor lungs were defined by specific criteria, including inflammatory infiltrates, pulmonary edema and partial pressure of arterial oxygen less than 300mmHg (inspired oxygen fraction of 100%). Results: During reperfusion, the mean partial pressure of arterial oxygen (inspired oxygen fraction of 100%) of the lungs did not change significantly (p=0.315). In the first hour, the mean partial pressure of arterial oxygen was 302.7mmHg (±127.66mmHg); in the second hour, 214.2mmHg (±94.12mmHg); in the third hour, 214.4mmHg (±99.70mmHg); and in the fourth hour, 217.7mmHg (±73.93mmHg). Plasma levels of lactate and glucose remained stable during perfusion, with no statistical difference between the moments studied (p=0.216). Conclusion: Ex vivo lung perfusion was reproduced in our center and ensured the preservation of lungs during the study period, which was 4 hours. The technique did not provide enough improvement for indicating organs for transplantation; therefore, it did not impact on use of these organs. |
| first_indexed | 2024-12-11T07:39:36Z |
| format | Article |
| id | doaj.art-c8c84251f1e6417fab1ce9db50e64baf |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-12-11T07:39:36Z |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-c8c84251f1e6417fab1ce9db50e64baf2022-12-22T01:15:37ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-638517410.31744/einstein_journal/2019ao4288S1679-45082019000400200Evaluation and reconditioning of donor organs for transplantation through ex vivo lung perfusionLuis Gustavo AbdallaKarina Andrighetti de Oliveira-BragaLucas Matos FernandesMarcos Naoyuki SamanoPaula Refinetti CameriniPaulo Manuel Pêgo-FernandesABSTRACT Objective: To assess the feasibility and impact of ex vivo lung perfusion with hyperoncotic solution (Steen Solution™) in the utilization of these organs in Brazil. Methods: In this prospective study, we subjected five lungs considered to be high risk for transplantation to 4 hours of ex vivo lung perfusion, with evaluation of oxygenation capacity. High-risk donor lungs were defined by specific criteria, including inflammatory infiltrates, pulmonary edema and partial pressure of arterial oxygen less than 300mmHg (inspired oxygen fraction of 100%). Results: During reperfusion, the mean partial pressure of arterial oxygen (inspired oxygen fraction of 100%) of the lungs did not change significantly (p=0.315). In the first hour, the mean partial pressure of arterial oxygen was 302.7mmHg (±127.66mmHg); in the second hour, 214.2mmHg (±94.12mmHg); in the third hour, 214.4mmHg (±99.70mmHg); and in the fourth hour, 217.7mmHg (±73.93mmHg). Plasma levels of lactate and glucose remained stable during perfusion, with no statistical difference between the moments studied (p=0.216). Conclusion: Ex vivo lung perfusion was reproduced in our center and ensured the preservation of lungs during the study period, which was 4 hours. The technique did not provide enough improvement for indicating organs for transplantation; therefore, it did not impact on use of these organs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082019000400200&lng=en&tlng=enLung transplantationOrgan preservationBrain death |
| spellingShingle | Luis Gustavo Abdalla Karina Andrighetti de Oliveira-Braga Lucas Matos Fernandes Marcos Naoyuki Samano Paula Refinetti Camerini Paulo Manuel Pêgo-Fernandes Evaluation and reconditioning of donor organs for transplantation through ex vivo lung perfusion Einstein (São Paulo) Lung transplantation Organ preservation Brain death |
| title | Evaluation and reconditioning of donor organs for transplantation through ex vivo lung perfusion |
| title_full | Evaluation and reconditioning of donor organs for transplantation through ex vivo lung perfusion |
| title_fullStr | Evaluation and reconditioning of donor organs for transplantation through ex vivo lung perfusion |
| title_full_unstemmed | Evaluation and reconditioning of donor organs for transplantation through ex vivo lung perfusion |
| title_short | Evaluation and reconditioning of donor organs for transplantation through ex vivo lung perfusion |
| title_sort | evaluation and reconditioning of donor organs for transplantation through ex vivo lung perfusion |
| topic | Lung transplantation Organ preservation Brain death |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082019000400200&lng=en&tlng=en |
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