Molecular Diversity of Candida albicans Isolated from Immuno-compromised Patients, Based on MLST Method
Background: As regards multilocus sequence typing (MLST) method directly analyze the polymorphism within DNA sequences; we performed the first nationwide study on the genotypic relationships of Candida albicans strains obtained from oropharynx and bronchoalveolar lavage (BAL) samples from immunocomp...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-10-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/3397 |
| _version_ | 1818876786394529792 |
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| author | Seyed Mohammad Hosein AFSARIAN Hamid BADALI Tahereh SHOKOHI Sohrab NAJAFIPOUR |
| author_facet | Seyed Mohammad Hosein AFSARIAN Hamid BADALI Tahereh SHOKOHI Sohrab NAJAFIPOUR |
| author_sort | Seyed Mohammad Hosein AFSARIAN |
| collection | DOAJ |
| description | Background: As regards multilocus sequence typing (MLST) method directly analyze the polymorphism within DNA sequences; we performed the first nationwide study on the genotypic relationships of Candida albicans strains obtained from oropharynx and bronchoalveolar lavage (BAL) samples from immunocompromised patients.
Methods: Fourteen epidemiologically unrelated clinical strains of C. albicans were obtained from three hospitals in Mazandaran Province, Iran (2006 to 2012) from seven patients with pulmonary infections and the rest with oropharyngeal samples of immunocompromised patients. Seven loci of housekeeping genes were sequenced for all fourteen isolates.
Results: MLST was applied to a subset of 14 unrelated isolates. Seventy-one (2.5%) nucleotide sites were found to be variable. Accordingly, 60 different alleles were identified in seven loci among the isolates, among which two new alleles were obtained. Furthermore, 12 independent diploid sequence types (DSTs) including five novel DSTs were identified. The fourteen unrelated isolates were placed in 10 clonal clusters (CC) while two isolates were singletons, by eBURST analysis. Most of the isolates belonged to CC461 of eBURST analysis from the clade 11 and two isolates assigned to CC172 from the clade 15.
Conclusion: Pathogen distribution and relatedness for determining the epidemiology of nosocomial infections is highly recommended for pathogen control methods. |
| first_indexed | 2024-12-19T13:47:55Z |
| format | Article |
| id | doaj.art-c8c99b44e4dd48639a9f9ca707eba813 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-19T13:47:55Z |
| publishDate | 2015-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-c8c99b44e4dd48639a9f9ca707eba8132022-12-21T20:18:49ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932015-10-014493296Molecular Diversity of Candida albicans Isolated from Immuno-compromised Patients, Based on MLST MethodSeyed Mohammad Hosein AFSARIANHamid BADALITahereh SHOKOHISohrab NAJAFIPOURBackground: As regards multilocus sequence typing (MLST) method directly analyze the polymorphism within DNA sequences; we performed the first nationwide study on the genotypic relationships of Candida albicans strains obtained from oropharynx and bronchoalveolar lavage (BAL) samples from immunocompromised patients. Methods: Fourteen epidemiologically unrelated clinical strains of C. albicans were obtained from three hospitals in Mazandaran Province, Iran (2006 to 2012) from seven patients with pulmonary infections and the rest with oropharyngeal samples of immunocompromised patients. Seven loci of housekeeping genes were sequenced for all fourteen isolates. Results: MLST was applied to a subset of 14 unrelated isolates. Seventy-one (2.5%) nucleotide sites were found to be variable. Accordingly, 60 different alleles were identified in seven loci among the isolates, among which two new alleles were obtained. Furthermore, 12 independent diploid sequence types (DSTs) including five novel DSTs were identified. The fourteen unrelated isolates were placed in 10 clonal clusters (CC) while two isolates were singletons, by eBURST analysis. Most of the isolates belonged to CC461 of eBURST analysis from the clade 11 and two isolates assigned to CC172 from the clade 15. Conclusion: Pathogen distribution and relatedness for determining the epidemiology of nosocomial infections is highly recommended for pathogen control methods.https://ijph.tums.ac.ir/index.php/ijph/article/view/3397MLSTCandida albicansGenetic diversityUnrelated strains |
| spellingShingle | Seyed Mohammad Hosein AFSARIAN Hamid BADALI Tahereh SHOKOHI Sohrab NAJAFIPOUR Molecular Diversity of Candida albicans Isolated from Immuno-compromised Patients, Based on MLST Method Iranian Journal of Public Health MLST Candida albicans Genetic diversity Unrelated strains |
| title | Molecular Diversity of Candida albicans Isolated from Immuno-compromised Patients, Based on MLST Method |
| title_full | Molecular Diversity of Candida albicans Isolated from Immuno-compromised Patients, Based on MLST Method |
| title_fullStr | Molecular Diversity of Candida albicans Isolated from Immuno-compromised Patients, Based on MLST Method |
| title_full_unstemmed | Molecular Diversity of Candida albicans Isolated from Immuno-compromised Patients, Based on MLST Method |
| title_short | Molecular Diversity of Candida albicans Isolated from Immuno-compromised Patients, Based on MLST Method |
| title_sort | molecular diversity of candida albicans isolated from immuno compromised patients based on mlst method |
| topic | MLST Candida albicans Genetic diversity Unrelated strains |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/3397 |
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