A simple and cost-effective setup for super-resolution localization microscopy
Abstract Single molecule localization microscopy (SMLM) has become a powerful imaging tool for biomedical research, but it is mostly available in imaging facilities and a small number of laboratories due to its high cost. Here, we evaluate the possibility of replacing high-cost components on standar...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-01606-6 |
| _version_ | 1818851084857245696 |
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| author | Hongqiang Ma Rao Fu Jianquan Xu Yang Liu |
| author_facet | Hongqiang Ma Rao Fu Jianquan Xu Yang Liu |
| author_sort | Hongqiang Ma |
| collection | DOAJ |
| description | Abstract Single molecule localization microscopy (SMLM) has become a powerful imaging tool for biomedical research, but it is mostly available in imaging facilities and a small number of laboratories due to its high cost. Here, we evaluate the possibility of replacing high-cost components on standard SMLM with appropriate low-cost alternatives and build a simple but high-performance super-resolution SMLM setup. Through numerical simulation and biological experiments, we demonstrate that our low-cost SMLM setup can yield similar localization precision and spatial resolution compared to the standard SMLM equipped with state-of-the-art components, but at a small fraction of their cost. Our low-cost SMLM setup can potentially serve as a routine laboratory microscope with high-performance super-resolution imaging capability. |
| first_indexed | 2024-12-19T06:59:24Z |
| format | Article |
| id | doaj.art-c8ca032fc7f24d4c8c5cf61a09b93b09 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T06:59:24Z |
| publishDate | 2017-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c8ca032fc7f24d4c8c5cf61a09b93b092022-12-21T20:31:27ZengNature PortfolioScientific Reports2045-23222017-05-01711910.1038/s41598-017-01606-6A simple and cost-effective setup for super-resolution localization microscopyHongqiang Ma0Rao Fu1Jianquan Xu2Yang Liu3Biomedical and Optical Imaging Laboratory, Departments of Medicine and Bioengineering, University of PittsburghBiomedical and Optical Imaging Laboratory, Departments of Medicine and Bioengineering, University of PittsburghBiomedical and Optical Imaging Laboratory, Departments of Medicine and Bioengineering, University of PittsburghBiomedical and Optical Imaging Laboratory, Departments of Medicine and Bioengineering, University of PittsburghAbstract Single molecule localization microscopy (SMLM) has become a powerful imaging tool for biomedical research, but it is mostly available in imaging facilities and a small number of laboratories due to its high cost. Here, we evaluate the possibility of replacing high-cost components on standard SMLM with appropriate low-cost alternatives and build a simple but high-performance super-resolution SMLM setup. Through numerical simulation and biological experiments, we demonstrate that our low-cost SMLM setup can yield similar localization precision and spatial resolution compared to the standard SMLM equipped with state-of-the-art components, but at a small fraction of their cost. Our low-cost SMLM setup can potentially serve as a routine laboratory microscope with high-performance super-resolution imaging capability.https://doi.org/10.1038/s41598-017-01606-6 |
| spellingShingle | Hongqiang Ma Rao Fu Jianquan Xu Yang Liu A simple and cost-effective setup for super-resolution localization microscopy Scientific Reports |
| title | A simple and cost-effective setup for super-resolution localization microscopy |
| title_full | A simple and cost-effective setup for super-resolution localization microscopy |
| title_fullStr | A simple and cost-effective setup for super-resolution localization microscopy |
| title_full_unstemmed | A simple and cost-effective setup for super-resolution localization microscopy |
| title_short | A simple and cost-effective setup for super-resolution localization microscopy |
| title_sort | simple and cost effective setup for super resolution localization microscopy |
| url | https://doi.org/10.1038/s41598-017-01606-6 |
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