Discrimination between the Two Closely Related Species of the Operational Group <i>B. amyloliquefaciens</i> Based on Whole-Cell Fatty Acid Profiling
(1) Background: <i>Bacillus velezensis</i> and <i>Bacillus amyloliquefaciens</i> are closely related members of the “operational group <i>B. amyloliquefaciens</i>”, a taxonomical unit above species level within the ”<i>Bacillus subtilis</i> species com...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/2/418 |
| _version_ | 1797477815422025728 |
|---|---|
| author | Thu Huynh Mónika Vörös Orsolya Kedves Adiyadolgor Turbat György Sipos Balázs Leitgeb László Kredics Csaba Vágvölgyi András Szekeres |
| author_facet | Thu Huynh Mónika Vörös Orsolya Kedves Adiyadolgor Turbat György Sipos Balázs Leitgeb László Kredics Csaba Vágvölgyi András Szekeres |
| author_sort | Thu Huynh |
| collection | DOAJ |
| description | (1) Background: <i>Bacillus velezensis</i> and <i>Bacillus amyloliquefaciens</i> are closely related members of the “operational group <i>B. amyloliquefaciens</i>”, a taxonomical unit above species level within the ”<i>Bacillus subtilis</i> species complex”. They have similar morphological, physiological, biochemical, phenotypic, and phylogenetic characteristics. Thus, separating these two taxa from each another has proven to be difficult to implement and could not be pushed easily into the line of routine analyses. (2) Methods: The aim of this study was to determine whether whole FAME profiling could be used to distinguish between these two species, using both type strains and environmental isolates. Initially, the classification was determined by partial sequences of the <i>gyrA</i> and <i>rpoB</i> genes and the classified isolates and type strains were considered as samples to develop the identification method, based on FAME profiles. (3) Results: The dissimilarities in 16:0, 17:0 iso, and 17:0 FA components have drawn a distinction between the two species and minor differences in FA 14:0, 15:0 iso, and 16:0 iso were also visible. The statistical analysis of the FA profiles confirmed that the two taxa can be distinguished into two separate groups, where the isolates are identified without misreading. (4) Conclusions: Our study proposes that the developed easy and fast-automated identification tool based on cellular FA profiles can be routinely applied to distinguish <i>B. velezensis</i> and <i>B. amyloliquefaciens</i>. |
| first_indexed | 2024-03-09T21:23:06Z |
| format | Article |
| id | doaj.art-c8ca9d8ea7f24d4b923696d252abb24f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:06Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c8ca9d8ea7f24d4b923696d252abb24f2023-11-23T21:15:53ZengMDPI AGMicroorganisms2076-26072022-02-0110241810.3390/microorganisms10020418Discrimination between the Two Closely Related Species of the Operational Group <i>B. amyloliquefaciens</i> Based on Whole-Cell Fatty Acid ProfilingThu Huynh0Mónika Vörös1Orsolya Kedves2Adiyadolgor Turbat3György Sipos4Balázs Leitgeb5László Kredics6Csaba Vágvölgyi7András Szekeres8Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryFunctional Genomics and Bioinformatics Group, Research Center for Forestry and Wood Industry, University of Sopron, Bajcsy-Zsilinszky Str. 4, H-9400 Sopron, HungaryInstitute of Biophysics, Biological Research Centre, Eötvös Loránd Research Network, Temesvári Krt. 62, H-6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, Hungary(1) Background: <i>Bacillus velezensis</i> and <i>Bacillus amyloliquefaciens</i> are closely related members of the “operational group <i>B. amyloliquefaciens</i>”, a taxonomical unit above species level within the ”<i>Bacillus subtilis</i> species complex”. They have similar morphological, physiological, biochemical, phenotypic, and phylogenetic characteristics. Thus, separating these two taxa from each another has proven to be difficult to implement and could not be pushed easily into the line of routine analyses. (2) Methods: The aim of this study was to determine whether whole FAME profiling could be used to distinguish between these two species, using both type strains and environmental isolates. Initially, the classification was determined by partial sequences of the <i>gyrA</i> and <i>rpoB</i> genes and the classified isolates and type strains were considered as samples to develop the identification method, based on FAME profiles. (3) Results: The dissimilarities in 16:0, 17:0 iso, and 17:0 FA components have drawn a distinction between the two species and minor differences in FA 14:0, 15:0 iso, and 16:0 iso were also visible. The statistical analysis of the FA profiles confirmed that the two taxa can be distinguished into two separate groups, where the isolates are identified without misreading. (4) Conclusions: Our study proposes that the developed easy and fast-automated identification tool based on cellular FA profiles can be routinely applied to distinguish <i>B. velezensis</i> and <i>B. amyloliquefaciens</i>.https://www.mdpi.com/2076-2607/10/2/418<i>Bacillus</i> taxonomy<i>Bacillus velezensis</i><i>Bacillus amyloliquefaciens</i>fatty acid profilingchemotaxonomy |
| spellingShingle | Thu Huynh Mónika Vörös Orsolya Kedves Adiyadolgor Turbat György Sipos Balázs Leitgeb László Kredics Csaba Vágvölgyi András Szekeres Discrimination between the Two Closely Related Species of the Operational Group <i>B. amyloliquefaciens</i> Based on Whole-Cell Fatty Acid Profiling Microorganisms <i>Bacillus</i> taxonomy <i>Bacillus velezensis</i> <i>Bacillus amyloliquefaciens</i> fatty acid profiling chemotaxonomy |
| title | Discrimination between the Two Closely Related Species of the Operational Group <i>B. amyloliquefaciens</i> Based on Whole-Cell Fatty Acid Profiling |
| title_full | Discrimination between the Two Closely Related Species of the Operational Group <i>B. amyloliquefaciens</i> Based on Whole-Cell Fatty Acid Profiling |
| title_fullStr | Discrimination between the Two Closely Related Species of the Operational Group <i>B. amyloliquefaciens</i> Based on Whole-Cell Fatty Acid Profiling |
| title_full_unstemmed | Discrimination between the Two Closely Related Species of the Operational Group <i>B. amyloliquefaciens</i> Based on Whole-Cell Fatty Acid Profiling |
| title_short | Discrimination between the Two Closely Related Species of the Operational Group <i>B. amyloliquefaciens</i> Based on Whole-Cell Fatty Acid Profiling |
| title_sort | discrimination between the two closely related species of the operational group i b amyloliquefaciens i based on whole cell fatty acid profiling |
| topic | <i>Bacillus</i> taxonomy <i>Bacillus velezensis</i> <i>Bacillus amyloliquefaciens</i> fatty acid profiling chemotaxonomy |
| url | https://www.mdpi.com/2076-2607/10/2/418 |
| work_keys_str_mv | AT thuhuynh discriminationbetweenthetwocloselyrelatedspeciesoftheoperationalgroupibamyloliquefaciensibasedonwholecellfattyacidprofiling AT monikavoros discriminationbetweenthetwocloselyrelatedspeciesoftheoperationalgroupibamyloliquefaciensibasedonwholecellfattyacidprofiling AT orsolyakedves discriminationbetweenthetwocloselyrelatedspeciesoftheoperationalgroupibamyloliquefaciensibasedonwholecellfattyacidprofiling AT adiyadolgorturbat discriminationbetweenthetwocloselyrelatedspeciesoftheoperationalgroupibamyloliquefaciensibasedonwholecellfattyacidprofiling AT gyorgysipos discriminationbetweenthetwocloselyrelatedspeciesoftheoperationalgroupibamyloliquefaciensibasedonwholecellfattyacidprofiling AT balazsleitgeb discriminationbetweenthetwocloselyrelatedspeciesoftheoperationalgroupibamyloliquefaciensibasedonwholecellfattyacidprofiling AT laszlokredics discriminationbetweenthetwocloselyrelatedspeciesoftheoperationalgroupibamyloliquefaciensibasedonwholecellfattyacidprofiling AT csabavagvolgyi discriminationbetweenthetwocloselyrelatedspeciesoftheoperationalgroupibamyloliquefaciensibasedonwholecellfattyacidprofiling AT andrasszekeres discriminationbetweenthetwocloselyrelatedspeciesoftheoperationalgroupibamyloliquefaciensibasedonwholecellfattyacidprofiling |