Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production
Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00045/full |
| _version_ | 1831696998681542656 |
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| author | Suvi T. Häkkinen Lauri Reuter Ninni Nuorti Jussi J. Joensuu Heiko Rischer Anneli Ritala |
| author_facet | Suvi T. Häkkinen Lauri Reuter Ninni Nuorti Jussi J. Joensuu Heiko Rischer Anneli Ritala |
| author_sort | Suvi T. Häkkinen |
| collection | DOAJ |
| description | Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the aim was to optimize the levels of most expensive components in the nutrient medium without compromising the accumulation of biomass and recombinant protein yields. Wild-type BY-2 culture and transgenic tobacco BY-2 expressing green fluorescent protein–Hydrophobin I (GFP-HFBI) fusion protein were used to determine the most inexpensive medium composition. One particularly high-accumulating BY-2 clone, named ‘Hulk,’ produced 1.1 ± 0.2 g/l GFP-HFBI in suspension and kept its high performance during prolonged subculturing. In addition, both cultures were successfully cryopreserved enabling truly industrial application of this plant cell host. With the optimized culture medium, 43–55% cost reduction with regard to biomass and up to 69% reduction with regard to recombinant protein production was achieved. |
| first_indexed | 2024-12-20T13:25:10Z |
| format | Article |
| id | doaj.art-c8cba93e82d74c379d1bd6740845304f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T13:25:10Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c8cba93e82d74c379d1bd6740845304f2022-12-21T19:39:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-01-01910.3389/fpls.2018.00045330489Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein ProductionSuvi T. HäkkinenLauri ReuterNinni NuortiJussi J. JoensuuHeiko RischerAnneli RitalaPlant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the aim was to optimize the levels of most expensive components in the nutrient medium without compromising the accumulation of biomass and recombinant protein yields. Wild-type BY-2 culture and transgenic tobacco BY-2 expressing green fluorescent protein–Hydrophobin I (GFP-HFBI) fusion protein were used to determine the most inexpensive medium composition. One particularly high-accumulating BY-2 clone, named ‘Hulk,’ produced 1.1 ± 0.2 g/l GFP-HFBI in suspension and kept its high performance during prolonged subculturing. In addition, both cultures were successfully cryopreserved enabling truly industrial application of this plant cell host. With the optimized culture medium, 43–55% cost reduction with regard to biomass and up to 69% reduction with regard to recombinant protein production was achieved.http://journal.frontiersin.org/article/10.3389/fpls.2018.00045/fulltobaccoBY-2recombinant proteincost evaluationGFP-hydrophobin I |
| spellingShingle | Suvi T. Häkkinen Lauri Reuter Ninni Nuorti Jussi J. Joensuu Heiko Rischer Anneli Ritala Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production Frontiers in Plant Science tobacco BY-2 recombinant protein cost evaluation GFP-hydrophobin I |
| title | Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production |
| title_full | Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production |
| title_fullStr | Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production |
| title_full_unstemmed | Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production |
| title_short | Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production |
| title_sort | tobacco by 2 media component optimization for a cost efficient recombinant protein production |
| topic | tobacco BY-2 recombinant protein cost evaluation GFP-hydrophobin I |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00045/full |
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