Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes
To obtain a clear understanding of the mode of action of potassium bicarbonate (PB), sodium silicate (SSi) and calcium chelate (CCh) solutions (1%) in inducing resistance to gray mold disease in table grapes, enzymatic and nonenzymatic investigations were carried out. In particular, changes in the a...
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MDPI AG
2020-09-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/6/3/179 |
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| author | Khamis Youssef Sergio Ruffo Roberto Angélica Nunes Tiepo Leonel Vinicius Constantino Juliano Tadeu Vilela de Resende Kamal A.M. Abo-Elyousr |
| author_facet | Khamis Youssef Sergio Ruffo Roberto Angélica Nunes Tiepo Leonel Vinicius Constantino Juliano Tadeu Vilela de Resende Kamal A.M. Abo-Elyousr |
| author_sort | Khamis Youssef |
| collection | DOAJ |
| description | To obtain a clear understanding of the mode of action of potassium bicarbonate (PB), sodium silicate (SSi) and calcium chelate (CCh) solutions (1%) in inducing resistance to gray mold disease in table grapes, enzymatic and nonenzymatic investigations were carried out. In particular, changes in the activity of the enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POD), total phenolic content and total flavonoid content were studied. As indirect action, PB, SSi and CCh reduced the incidence of gray mold by 43%, 50% and 41%, respectively. The highest activity of SOD was detected at 48 h in SSi-treated tissue, PB-treated tissue and CCh-treated tissue, and it was 1.7-, 1.4- and 1.2-fold higher, respectively, compared to the control. The APX activity was significantly higher in SSi-treated tissue than in the control at 24, 48 and 72 h and showed an increase in activity 2-fold for all times. Additionally, PB, SSi and CCh increased the activity of POD by 1.4-, 1.2- and 2.7-fold at 48 h posttreatment, respectively. The results showed that CCh was the most pronounced salt to increase both total phenol and flavonoid contents by 1.3 and 2.1, respectively. Additionally, the three tested salts induced an increase in total phenols and total flavonoids at 48 h posttreatment. The obtained result is one more movement towards an overall understanding of the mechanism by which salt solutions act as antimicrobial agents against gray mold of table grapes. |
| first_indexed | 2024-03-10T16:13:56Z |
| format | Article |
| id | doaj.art-c8d5840f9d62418490c3a10fc11a8b15 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T16:13:56Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-c8d5840f9d62418490c3a10fc11a8b152023-11-20T14:16:16ZengMDPI AGJournal of Fungi2309-608X2020-09-016317910.3390/jof6030179Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table GrapesKhamis Youssef0Sergio Ruffo Roberto1Angélica Nunes Tiepo2Leonel Vinicius Constantino3Juliano Tadeu Vilela de Resende4Kamal A.M. Abo-Elyousr5Agricultural Research Center, Plant Pathology Research Institute, 9 Gamaa St., Giza 12619, EgyptDepartment of Agronomy, Agricultural Research Center, Londrina State University, Londrina 86057-970, BrazilDepartment of Animal and Plant Biology, CCB, Londrina State University, Londrina 86057-970, BrazilDepartment of Agronomy, Agricultural Research Center, Londrina State University, Londrina 86057-970, BrazilDepartment of Agronomy, Agricultural Research Center, Londrina State University, Londrina 86057-970, BrazilDepartment of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 80208, Saudi ArabiaTo obtain a clear understanding of the mode of action of potassium bicarbonate (PB), sodium silicate (SSi) and calcium chelate (CCh) solutions (1%) in inducing resistance to gray mold disease in table grapes, enzymatic and nonenzymatic investigations were carried out. In particular, changes in the activity of the enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POD), total phenolic content and total flavonoid content were studied. As indirect action, PB, SSi and CCh reduced the incidence of gray mold by 43%, 50% and 41%, respectively. The highest activity of SOD was detected at 48 h in SSi-treated tissue, PB-treated tissue and CCh-treated tissue, and it was 1.7-, 1.4- and 1.2-fold higher, respectively, compared to the control. The APX activity was significantly higher in SSi-treated tissue than in the control at 24, 48 and 72 h and showed an increase in activity 2-fold for all times. Additionally, PB, SSi and CCh increased the activity of POD by 1.4-, 1.2- and 2.7-fold at 48 h posttreatment, respectively. The results showed that CCh was the most pronounced salt to increase both total phenol and flavonoid contents by 1.3 and 2.1, respectively. Additionally, the three tested salts induced an increase in total phenols and total flavonoids at 48 h posttreatment. The obtained result is one more movement towards an overall understanding of the mechanism by which salt solutions act as antimicrobial agents against gray mold of table grapes.https://www.mdpi.com/2309-608X/6/3/179saltmechanism of actionantioxidant enzyme<i>Botrytis cinerea</i> |
| spellingShingle | Khamis Youssef Sergio Ruffo Roberto Angélica Nunes Tiepo Leonel Vinicius Constantino Juliano Tadeu Vilela de Resende Kamal A.M. Abo-Elyousr Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes Journal of Fungi salt mechanism of action antioxidant enzyme <i>Botrytis cinerea</i> |
| title | Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes |
| title_full | Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes |
| title_fullStr | Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes |
| title_full_unstemmed | Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes |
| title_short | Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes |
| title_sort | salt solution treatments trigger antioxidant defense response against gray mold disease in table grapes |
| topic | salt mechanism of action antioxidant enzyme <i>Botrytis cinerea</i> |
| url | https://www.mdpi.com/2309-608X/6/3/179 |
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