A high-throughput microfluidic diploid yeast long-term culturing (DYLC) chip capable of bud reorientation and concerted daughter dissection for replicative lifespan determination

A high-throughput microfluidic diploid yeast long-term culturing (DYLC) chip capable of bud reorientation and concerted daughter dissection for replicative lifespan determination

Abstract Background Budding yeast, Saccharomyces cerevisiae, has been extensively favored as a model organism in aging and age-related studies, thanks to versatile microfluidic chips for cell dynamics assay and replicative lifespan (RLS) determination at single-cell resolution. However, previous mic...

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Bibliographic Details
Main Authors: Yingying Wang, Zhen Zhu, Ke Liu, Qin Xiao, Yangye Geng, Feng Xu, Shuiping Ouyang, Ke Zheng, Yimin Fan, Nan Jin, Xiangwei Zhao, Mario A. Marchisio, Dejing Pan, Qing-an Huang
Format: Article
Language:English
Published: BMC 2022-03-01
Series:Journal of Nanobiotechnology
Subjects:
Microfluidics
Single-cell analysis
Saccharomyces cerevisiae
Diploid yeast aging
Daughter-cell dissection
Replicative lifespan
Online Access:https://doi.org/10.1186/s12951-022-01379-9

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