Application of Freeze-Dryers of Chamber Type in the Collections of Pathogenic Microorganisms
By the example of Martin Christ Epsilon 2-6D device carried out was assessment of the possibility to use freeze-dryers of the chamber type for conservation of pathogenic microorganisms collection strains. Elaborated was algorithm of lyophilisation of the III-IV pathogenicity groups bacteria, which i...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2014-09-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/166 |
| _version_ | 1797220896947044352 |
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| author | N. S. Chervyakova T. V. Valova A. V. Osin |
| author_facet | N. S. Chervyakova T. V. Valova A. V. Osin |
| author_sort | N. S. Chervyakova |
| collection | DOAJ |
| description | By the example of Martin Christ Epsilon 2-6D device carried out was assessment of the possibility to use freeze-dryers of the chamber type for conservation of pathogenic microorganisms collection strains. Elaborated was algorithm of lyophilisation of the III-IV pathogenicity groups bacteria, which incorporated conditions of freeze-drying and biological safety provision of this process. Indices of viability and survivability were defined for freeze-dried cells of pathogenic bacteria strains. Using thermostability test calculated were predicted timelines of storage of collection strains preparations freeze-dried in the flasks in Martin Christ Epsilon 2-6D. It was determined that in the collections of pathogenic microorganisms freeze-dryers of the chamber type could be used most prospectively for the III-IV pathogenicity groups bacteria conservation requiring mass reproduction and not intended for long storage. At the same time their application for freeze-drying of the strains of the I-II pathogenicity groups bacteria intended for a long storage, requires further adaptation of these devices as regards biological safety provision and prolongation of the shelf life. |
| first_indexed | 2024-03-08T13:43:48Z |
| format | Article |
| id | doaj.art-c8d6c7e5f55049da9d82f1a1be1a22dd |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:56:50Z |
| publishDate | 2014-09-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-c8d6c7e5f55049da9d82f1a1be1a22dd2024-04-05T16:47:10ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2014-09-0103656810.21055/0370-1069-2014-3-65-68166Application of Freeze-Dryers of Chamber Type in the Collections of Pathogenic MicroorganismsN. S. Chervyakova0T. V. Valova1A. V. Osin2Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”By the example of Martin Christ Epsilon 2-6D device carried out was assessment of the possibility to use freeze-dryers of the chamber type for conservation of pathogenic microorganisms collection strains. Elaborated was algorithm of lyophilisation of the III-IV pathogenicity groups bacteria, which incorporated conditions of freeze-drying and biological safety provision of this process. Indices of viability and survivability were defined for freeze-dried cells of pathogenic bacteria strains. Using thermostability test calculated were predicted timelines of storage of collection strains preparations freeze-dried in the flasks in Martin Christ Epsilon 2-6D. It was determined that in the collections of pathogenic microorganisms freeze-dryers of the chamber type could be used most prospectively for the III-IV pathogenicity groups bacteria conservation requiring mass reproduction and not intended for long storage. At the same time their application for freeze-drying of the strains of the I-II pathogenicity groups bacteria intended for a long storage, requires further adaptation of these devices as regards biological safety provision and prolongation of the shelf life.https://journal.microbe.ru/jour/article/view/166коллекционные штаммылиофилизациявыживаемость клетокпрогнозируемый срок храненияcollection strainsfreeze-dryingsurvivability of cellspredicted shelf life |
| spellingShingle | N. S. Chervyakova T. V. Valova A. V. Osin Application of Freeze-Dryers of Chamber Type in the Collections of Pathogenic Microorganisms Проблемы особо опасных инфекций коллекционные штаммы лиофилизация выживаемость клеток прогнозируемый срок хранения collection strains freeze-drying survivability of cells predicted shelf life |
| title | Application of Freeze-Dryers of Chamber Type in the Collections of Pathogenic Microorganisms |
| title_full | Application of Freeze-Dryers of Chamber Type in the Collections of Pathogenic Microorganisms |
| title_fullStr | Application of Freeze-Dryers of Chamber Type in the Collections of Pathogenic Microorganisms |
| title_full_unstemmed | Application of Freeze-Dryers of Chamber Type in the Collections of Pathogenic Microorganisms |
| title_short | Application of Freeze-Dryers of Chamber Type in the Collections of Pathogenic Microorganisms |
| title_sort | application of freeze dryers of chamber type in the collections of pathogenic microorganisms |
| topic | коллекционные штаммы лиофилизация выживаемость клеток прогнозируемый срок хранения collection strains freeze-drying survivability of cells predicted shelf life |
| url | https://journal.microbe.ru/jour/article/view/166 |
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