Exploring the antibiogram of soil isolates from an indian hospital precinct: link to antibiotic usage
Abstract Objective Hospitals serve as hotspots of antibiotic resistance. Despite several studies exploring antibiotic resistance in hospitals, none have explored the resistance profile of soil bacteria from a hospital precinct. This study examined and compared the antibiogram of the soil isolates fr...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-08-01
|
| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-023-06450-8 |
| _version_ | 1797454045933207552 |
|---|---|
| author | Shalini Kunhikannan Colleen J. Thomas M. N. Sumana Ashley E. Franks Sumana Kumar S. Nagarathna Steve Petrovski Anya E. Shindler |
| author_facet | Shalini Kunhikannan Colleen J. Thomas M. N. Sumana Ashley E. Franks Sumana Kumar S. Nagarathna Steve Petrovski Anya E. Shindler |
| author_sort | Shalini Kunhikannan |
| collection | DOAJ |
| description | Abstract Objective Hospitals serve as hotspots of antibiotic resistance. Despite several studies exploring antibiotic resistance in hospitals, none have explored the resistance profile of soil bacteria from a hospital precinct. This study examined and compared the antibiogram of the soil isolates from a hospital and its affiliated university precinct, to determine if antibiotic resistant bacteria were present closer to the hospital. Results 120 soil samples were collected from JSS Hospital and JSS University in Mysore, India across three consecutive seasons (monsoon, winter and summer). 366 isolates were randomly selected from culture. Antibiotic susceptibility testing was performed on 128 isolates of Pseudomonas (n = 73), Acinetobacter (n = 30), Klebsiella species (n = 15) and Escherichia coli (n = 10). Pseudomonas species exhibited the highest antibiotic resistance. Ticarcillin-clavulanic acid, an extended-spectrum carboxypenicillin antibiotic used to treat moderate-to-severe infections, ranked highest amongst the antibiotics to whom these isolates were resistant (n = 51 out of 73, 69.9%). Moreover, 56.8% (n = 29) were from the hospital and 43.1% (n = 22) were from the university precinct, indicating antibiotic resistant bacteria were closer to the hospital setting. This study highlights the effect of antibiotic usage in hospitals and the influence of anthropogenic activities in the hospital on the dissemination of antibiotic resistance into hospital precinct soil. |
| first_indexed | 2024-03-09T15:31:36Z |
| format | Article |
| id | doaj.art-c8d8968c5c894de1be36941083c3a9d7 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-03-09T15:31:36Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-c8d8968c5c894de1be36941083c3a9d72023-11-26T12:14:16ZengBMCBMC Research Notes1756-05002023-08-0116111110.1186/s13104-023-06450-8Exploring the antibiogram of soil isolates from an indian hospital precinct: link to antibiotic usageShalini Kunhikannan0Colleen J. Thomas1M. N. Sumana2Ashley E. Franks3Sumana Kumar4S. Nagarathna5Steve Petrovski6Anya E. Shindler7Department of Microbiology, Anatomy, Physiology and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe UniversityDepartment of Microbiology, Anatomy, Physiology and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe UniversityDepartment of Microbiology, JSS Medical College and HospitalDepartment of Microbiology, Anatomy, Physiology and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe UniversityDepartment of Microbiology, Faculty of Life Sciences, JSS Academy of Higher Education and ResearchProfessor and Head, National Institute of Mental Health and NeurosciencesDepartment of Microbiology, Anatomy, Physiology and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe UniversityDepartment of Microbiology, Anatomy, Physiology and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe UniversityAbstract Objective Hospitals serve as hotspots of antibiotic resistance. Despite several studies exploring antibiotic resistance in hospitals, none have explored the resistance profile of soil bacteria from a hospital precinct. This study examined and compared the antibiogram of the soil isolates from a hospital and its affiliated university precinct, to determine if antibiotic resistant bacteria were present closer to the hospital. Results 120 soil samples were collected from JSS Hospital and JSS University in Mysore, India across three consecutive seasons (monsoon, winter and summer). 366 isolates were randomly selected from culture. Antibiotic susceptibility testing was performed on 128 isolates of Pseudomonas (n = 73), Acinetobacter (n = 30), Klebsiella species (n = 15) and Escherichia coli (n = 10). Pseudomonas species exhibited the highest antibiotic resistance. Ticarcillin-clavulanic acid, an extended-spectrum carboxypenicillin antibiotic used to treat moderate-to-severe infections, ranked highest amongst the antibiotics to whom these isolates were resistant (n = 51 out of 73, 69.9%). Moreover, 56.8% (n = 29) were from the hospital and 43.1% (n = 22) were from the university precinct, indicating antibiotic resistant bacteria were closer to the hospital setting. This study highlights the effect of antibiotic usage in hospitals and the influence of anthropogenic activities in the hospital on the dissemination of antibiotic resistance into hospital precinct soil.https://doi.org/10.1186/s13104-023-06450-8Antibiotic resistanceHospitalsPseudomonasSoilTicarcillin-clavulanic acid |
| spellingShingle | Shalini Kunhikannan Colleen J. Thomas M. N. Sumana Ashley E. Franks Sumana Kumar S. Nagarathna Steve Petrovski Anya E. Shindler Exploring the antibiogram of soil isolates from an indian hospital precinct: link to antibiotic usage BMC Research Notes Antibiotic resistance Hospitals Pseudomonas Soil Ticarcillin-clavulanic acid |
| title | Exploring the antibiogram of soil isolates from an indian hospital precinct: link to antibiotic usage |
| title_full | Exploring the antibiogram of soil isolates from an indian hospital precinct: link to antibiotic usage |
| title_fullStr | Exploring the antibiogram of soil isolates from an indian hospital precinct: link to antibiotic usage |
| title_full_unstemmed | Exploring the antibiogram of soil isolates from an indian hospital precinct: link to antibiotic usage |
| title_short | Exploring the antibiogram of soil isolates from an indian hospital precinct: link to antibiotic usage |
| title_sort | exploring the antibiogram of soil isolates from an indian hospital precinct link to antibiotic usage |
| topic | Antibiotic resistance Hospitals Pseudomonas Soil Ticarcillin-clavulanic acid |
| url | https://doi.org/10.1186/s13104-023-06450-8 |
| work_keys_str_mv | AT shalinikunhikannan exploringtheantibiogramofsoilisolatesfromanindianhospitalprecinctlinktoantibioticusage AT colleenjthomas exploringtheantibiogramofsoilisolatesfromanindianhospitalprecinctlinktoantibioticusage AT mnsumana exploringtheantibiogramofsoilisolatesfromanindianhospitalprecinctlinktoantibioticusage AT ashleyefranks exploringtheantibiogramofsoilisolatesfromanindianhospitalprecinctlinktoantibioticusage AT sumanakumar exploringtheantibiogramofsoilisolatesfromanindianhospitalprecinctlinktoantibioticusage AT snagarathna exploringtheantibiogramofsoilisolatesfromanindianhospitalprecinctlinktoantibioticusage AT stevepetrovski exploringtheantibiogramofsoilisolatesfromanindianhospitalprecinctlinktoantibioticusage AT anyaeshindler exploringtheantibiogramofsoilisolatesfromanindianhospitalprecinctlinktoantibioticusage |