A localized surface plasmon resonance based immunosensor for the detection of casein in milk
In this research, a localized surface plasmon resonance (LSPR) immunosensor based on gold-capped nanoparticle substrate for detecting casein, one of the most potent allergens in milk, was developed. The fabrication of the gold-capped nanoparticle substrate involved a surface-modified silica nanopart...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2007-01-01
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| Series: | Science and Technology of Advanced Materials |
| Online Access: | http://www.iop.org/EJ/abstract/1468-6996/8/4/A16 |
| _version_ | 1811303882442145792 |
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| author | Ha Minh Hiep et al |
| author_facet | Ha Minh Hiep et al |
| author_sort | Ha Minh Hiep et al |
| collection | DOAJ |
| description | In this research, a localized surface plasmon resonance (LSPR) immunosensor based on gold-capped nanoparticle substrate for detecting casein, one of the most potent allergens in milk, was developed. The fabrication of the gold-capped nanoparticle substrate involved a surface-modified silica nanoparticle layer (core) on the slide glass substrate between bottom and top gold layers (shell). The absorbance peak of the gold-capped nanoparticle substrate was observed at ~520 nm. In addition, the atomic force microscopy (AFM) images demonstrated that the nanoparticles formed a monolayer on the slide glass. After immobilizing anti-casein antibody on the surface, our device, casein immunosensor, could be applied easily for the detection of casein in the raw milk sample without a difficult pretreatment. Under the optimum conditions, the detection limit of the casein immunosensor was determined as 10 ng/mL. Our device brings several advantages to the existing LSPR-based biosensors with its easy fabrication, simple handling, low-cost, and high sensitivity. |
| first_indexed | 2024-04-13T07:56:11Z |
| format | Article |
| id | doaj.art-c8daefb2ef494ac0993bee332d76cf4c |
| institution | Directory Open Access Journal |
| issn | 1468-6996 1878-5514 |
| language | English |
| last_indexed | 2024-04-13T07:56:11Z |
| publishDate | 2007-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Advanced Materials |
| spelling | doaj.art-c8daefb2ef494ac0993bee332d76cf4c2022-12-22T02:55:24ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142007-01-0184331A localized surface plasmon resonance based immunosensor for the detection of casein in milkHa Minh Hiep et alIn this research, a localized surface plasmon resonance (LSPR) immunosensor based on gold-capped nanoparticle substrate for detecting casein, one of the most potent allergens in milk, was developed. The fabrication of the gold-capped nanoparticle substrate involved a surface-modified silica nanoparticle layer (core) on the slide glass substrate between bottom and top gold layers (shell). The absorbance peak of the gold-capped nanoparticle substrate was observed at ~520 nm. In addition, the atomic force microscopy (AFM) images demonstrated that the nanoparticles formed a monolayer on the slide glass. After immobilizing anti-casein antibody on the surface, our device, casein immunosensor, could be applied easily for the detection of casein in the raw milk sample without a difficult pretreatment. Under the optimum conditions, the detection limit of the casein immunosensor was determined as 10 ng/mL. Our device brings several advantages to the existing LSPR-based biosensors with its easy fabrication, simple handling, low-cost, and high sensitivity.http://www.iop.org/EJ/abstract/1468-6996/8/4/A16 |
| spellingShingle | Ha Minh Hiep et al A localized surface plasmon resonance based immunosensor for the detection of casein in milk Science and Technology of Advanced Materials |
| title | A localized surface plasmon resonance based immunosensor for the detection of casein in milk |
| title_full | A localized surface plasmon resonance based immunosensor for the detection of casein in milk |
| title_fullStr | A localized surface plasmon resonance based immunosensor for the detection of casein in milk |
| title_full_unstemmed | A localized surface plasmon resonance based immunosensor for the detection of casein in milk |
| title_short | A localized surface plasmon resonance based immunosensor for the detection of casein in milk |
| title_sort | localized surface plasmon resonance based immunosensor for the detection of casein in milk |
| url | http://www.iop.org/EJ/abstract/1468-6996/8/4/A16 |
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