Maternal total cell-free DNA in preeclampsia with and without intrauterine growth restriction
Abstract Elevation of total cell-free DNA (cfDNA) in patients with preeclampsia is well-known; however, whether this change precedes the onset of symptoms remains inconclusive. Here, we conducted a nested case–control study to determine the elevation of cfDNA levels in women who subsequently develop...
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Nature Portfolio
2020-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-68842-1 |
| _version_ | 1818996769992736768 |
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| author | Dong Wook Kwak Shin Young Kim Hyun Jin Kim Ji Hyae Lim Young-Han Kim Hyun Mee Ryu |
| author_facet | Dong Wook Kwak Shin Young Kim Hyun Jin Kim Ji Hyae Lim Young-Han Kim Hyun Mee Ryu |
| author_sort | Dong Wook Kwak |
| collection | DOAJ |
| description | Abstract Elevation of total cell-free DNA (cfDNA) in patients with preeclampsia is well-known; however, whether this change precedes the onset of symptoms remains inconclusive. Here, we conducted a nested case–control study to determine the elevation of cfDNA levels in women who subsequently developed preeclampsia. Methylated HYP2 (m-HYP2) levels were determined in 68 blood samples collected from women with hypertensive disorders of pregnancy, along with 136 control samples, using real-time quantitative PCR. The measured m-HYP2 levels were converted to multiples of the median (MoM) values for correction of maternal characteristics. The m-HYP2 levels and MoM values in patients with preeclampsia were significantly higher than in controls during the third trimester (P < 0.001, both), whereas those for women who subsequently developed preeclampsia did not differ during the second trimester. However, when patients with preeclampsia were divided based on the onset-time of preeclampsia or 10th percentile birth weight, both values were significantly higher in women who subsequently developed early-onset preeclampsia (P < 0.05, both) and preeclampsia with small-for-gestational-age (SGA) neonate (P < 0.01, both) than controls. These results suggested that total cfDNA levels could be used to predict early-onset preeclampsia or preeclampsia with SGA neonate. |
| first_indexed | 2024-12-20T21:35:01Z |
| format | Article |
| id | doaj.art-c8dd70c1ea094b8a98d6e721f0060274 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:35:01Z |
| publishDate | 2020-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c8dd70c1ea094b8a98d6e721f00602742022-12-21T19:25:57ZengNature PortfolioScientific Reports2045-23222020-07-011011810.1038/s41598-020-68842-1Maternal total cell-free DNA in preeclampsia with and without intrauterine growth restrictionDong Wook Kwak0Shin Young Kim1Hyun Jin Kim2Ji Hyae Lim3Young-Han Kim4Hyun Mee Ryu5Department of Obstetrics and Gynecology, Ajou University School of MedicineDepartment of Obstetrics and Gynecology, Cheil General Hospital and Women’s Healthcare CenterDepartment of Obstetrics and Gynecology, CHA Gangnam Medical CenterCenter for Prenatal Biomarker Research, CHA Advanced Research InstituteDivision of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Institute of Women’s Life Medical Science, Yonsei University College of MedicineCenter for Prenatal Biomarker Research, CHA Advanced Research InstituteAbstract Elevation of total cell-free DNA (cfDNA) in patients with preeclampsia is well-known; however, whether this change precedes the onset of symptoms remains inconclusive. Here, we conducted a nested case–control study to determine the elevation of cfDNA levels in women who subsequently developed preeclampsia. Methylated HYP2 (m-HYP2) levels were determined in 68 blood samples collected from women with hypertensive disorders of pregnancy, along with 136 control samples, using real-time quantitative PCR. The measured m-HYP2 levels were converted to multiples of the median (MoM) values for correction of maternal characteristics. The m-HYP2 levels and MoM values in patients with preeclampsia were significantly higher than in controls during the third trimester (P < 0.001, both), whereas those for women who subsequently developed preeclampsia did not differ during the second trimester. However, when patients with preeclampsia were divided based on the onset-time of preeclampsia or 10th percentile birth weight, both values were significantly higher in women who subsequently developed early-onset preeclampsia (P < 0.05, both) and preeclampsia with small-for-gestational-age (SGA) neonate (P < 0.01, both) than controls. These results suggested that total cfDNA levels could be used to predict early-onset preeclampsia or preeclampsia with SGA neonate.https://doi.org/10.1038/s41598-020-68842-1 |
| spellingShingle | Dong Wook Kwak Shin Young Kim Hyun Jin Kim Ji Hyae Lim Young-Han Kim Hyun Mee Ryu Maternal total cell-free DNA in preeclampsia with and without intrauterine growth restriction Scientific Reports |
| title | Maternal total cell-free DNA in preeclampsia with and without intrauterine growth restriction |
| title_full | Maternal total cell-free DNA in preeclampsia with and without intrauterine growth restriction |
| title_fullStr | Maternal total cell-free DNA in preeclampsia with and without intrauterine growth restriction |
| title_full_unstemmed | Maternal total cell-free DNA in preeclampsia with and without intrauterine growth restriction |
| title_short | Maternal total cell-free DNA in preeclampsia with and without intrauterine growth restriction |
| title_sort | maternal total cell free dna in preeclampsia with and without intrauterine growth restriction |
| url | https://doi.org/10.1038/s41598-020-68842-1 |
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