Effect of Inoculum Size and Age, and Sucrose Concentration on Cell Growth to Promote Metabolites Production in Cultured <i>Taraxacum officinale</i> (Weber) Cells
Pentacyclic triterpenes, including lupeol, α- amyrin, and β-amyrin, present a large range of biological activities including anti-inflammatory, anti-cancer, and gastroprotective properties. The phytochemistry of dandelion (<i>Taraxacum officinale</i>) tissues has been widely described. P...
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MDPI AG
2023-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/5/1116 |
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| author | María Eugenia Martínez Lorena Jorquera Paola Poirrier Katy Díaz Rolando Chamy |
| author_facet | María Eugenia Martínez Lorena Jorquera Paola Poirrier Katy Díaz Rolando Chamy |
| author_sort | María Eugenia Martínez |
| collection | DOAJ |
| description | Pentacyclic triterpenes, including lupeol, α- amyrin, and β-amyrin, present a large range of biological activities including anti-inflammatory, anti-cancer, and gastroprotective properties. The phytochemistry of dandelion (<i>Taraxacum officinale</i>) tissues has been widely described. Plant biotechnology offers an alternative for secondary metabolite production and several active plant ingredients are already synthesized through in vitro cultures. This study aimed to establish a suitable protocol for cell growth and to determine the accumulation of α-amyrin and lupeol in cell suspension cultures of <i>T. officinale</i> under different culture conditions. To this end, inoculum density (0.2% to 8% (<i>w</i>/<i>v</i>)), inoculum age (2- to 10-week-old), and carbon source concentration (1%, 2.3%, 3.2%, and 5.5% (<i>w</i>/<i>v</i>)) were investigated. Hypocotyl explants of <i>T. officinale</i> were used for callus induction. Age, size, and sucrose concentrations were statistically significant in cell growth (fresh and dry weight), cell quality (aggregation, differentiation, viability), and triterpenes yield. The best conditions for establishing a suspension culture were achieved by using a 6-week-old callus at 4% (<i>w</i>/<i>v</i>) and 1% (<i>w</i>/<i>v</i>) of sucrose concentration. Results indicate that 0.04 (±0.02) α-amyrin and 0.03 (±0.01) mg/g lupeol can be obtained in suspension culture under these starting conditions at the 8th week of culture. The results of the present study provide a backdrop for future studies in which an elicitor could be incorporated to increase the large-scale production of α-amyrin and lupeol from <i>T. officinale</i>. |
| first_indexed | 2024-03-11T07:13:59Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T07:13:59Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-c8e5a6c606314f19bc1768ba54f076c32023-11-17T08:24:23ZengMDPI AGPlants2223-77472023-03-01125111610.3390/plants12051116Effect of Inoculum Size and Age, and Sucrose Concentration on Cell Growth to Promote Metabolites Production in Cultured <i>Taraxacum officinale</i> (Weber) CellsMaría Eugenia Martínez0Lorena Jorquera1Paola Poirrier2Katy Díaz3Rolando Chamy4Escuela de Ingeniería Bioquímica, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, Brasil 2085, Valparaíso 237463, ChileEscuela de Ingeniería en Construcción y Transporte, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2147, Valparaíso 237463, ChileEscuela de Ingeniería Bioquímica, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, Brasil 2085, Valparaíso 237463, ChileDepartamento de Química, Universidad Técnica Federico Santa María, Avenida España #1680, Valparaíso 2390123, ChileEscuela de Ingeniería Bioquímica, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, Brasil 2085, Valparaíso 237463, ChilePentacyclic triterpenes, including lupeol, α- amyrin, and β-amyrin, present a large range of biological activities including anti-inflammatory, anti-cancer, and gastroprotective properties. The phytochemistry of dandelion (<i>Taraxacum officinale</i>) tissues has been widely described. Plant biotechnology offers an alternative for secondary metabolite production and several active plant ingredients are already synthesized through in vitro cultures. This study aimed to establish a suitable protocol for cell growth and to determine the accumulation of α-amyrin and lupeol in cell suspension cultures of <i>T. officinale</i> under different culture conditions. To this end, inoculum density (0.2% to 8% (<i>w</i>/<i>v</i>)), inoculum age (2- to 10-week-old), and carbon source concentration (1%, 2.3%, 3.2%, and 5.5% (<i>w</i>/<i>v</i>)) were investigated. Hypocotyl explants of <i>T. officinale</i> were used for callus induction. Age, size, and sucrose concentrations were statistically significant in cell growth (fresh and dry weight), cell quality (aggregation, differentiation, viability), and triterpenes yield. The best conditions for establishing a suspension culture were achieved by using a 6-week-old callus at 4% (<i>w</i>/<i>v</i>) and 1% (<i>w</i>/<i>v</i>) of sucrose concentration. Results indicate that 0.04 (±0.02) α-amyrin and 0.03 (±0.01) mg/g lupeol can be obtained in suspension culture under these starting conditions at the 8th week of culture. The results of the present study provide a backdrop for future studies in which an elicitor could be incorporated to increase the large-scale production of α-amyrin and lupeol from <i>T. officinale</i>.https://www.mdpi.com/2223-7747/12/5/1116dandeliontriterpenesecondary metabolitecell suspensionlupeolα-amyrin |
| spellingShingle | María Eugenia Martínez Lorena Jorquera Paola Poirrier Katy Díaz Rolando Chamy Effect of Inoculum Size and Age, and Sucrose Concentration on Cell Growth to Promote Metabolites Production in Cultured <i>Taraxacum officinale</i> (Weber) Cells Plants dandelion triterpene secondary metabolite cell suspension lupeol α-amyrin |
| title | Effect of Inoculum Size and Age, and Sucrose Concentration on Cell Growth to Promote Metabolites Production in Cultured <i>Taraxacum officinale</i> (Weber) Cells |
| title_full | Effect of Inoculum Size and Age, and Sucrose Concentration on Cell Growth to Promote Metabolites Production in Cultured <i>Taraxacum officinale</i> (Weber) Cells |
| title_fullStr | Effect of Inoculum Size and Age, and Sucrose Concentration on Cell Growth to Promote Metabolites Production in Cultured <i>Taraxacum officinale</i> (Weber) Cells |
| title_full_unstemmed | Effect of Inoculum Size and Age, and Sucrose Concentration on Cell Growth to Promote Metabolites Production in Cultured <i>Taraxacum officinale</i> (Weber) Cells |
| title_short | Effect of Inoculum Size and Age, and Sucrose Concentration on Cell Growth to Promote Metabolites Production in Cultured <i>Taraxacum officinale</i> (Weber) Cells |
| title_sort | effect of inoculum size and age and sucrose concentration on cell growth to promote metabolites production in cultured i taraxacum officinale i weber cells |
| topic | dandelion triterpene secondary metabolite cell suspension lupeol α-amyrin |
| url | https://www.mdpi.com/2223-7747/12/5/1116 |
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