PLGA nanoparticles as a platform for vitamin D-based cancer therapy
Poly(lactic-co-glycolic acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-H...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Beilstein-Institut
2015-06-01
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| Series: | Beilstein Journal of Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjnano.6.135 |
| _version_ | 1819076956870672384 |
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| author | Maria J. Ramalho Joana A. Loureiro Bárbara Gomes Manuela F. Frasco Manuel A. N. Coelho M. Carmo Pereira |
| author_facet | Maria J. Ramalho Joana A. Loureiro Bárbara Gomes Manuela F. Frasco Manuel A. N. Coelho M. Carmo Pereira |
| author_sort | Maria J. Ramalho |
| collection | DOAJ |
| description | Poly(lactic-co-glycolic acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-HPNE, and the lung cancer cell line A549. Encapsulated calcitriol retained its biological activity, reducing the cell growth. Cytotoxicity assays demonstrated that encapsulation of calcitriol enhanced its inhibitory effect on cell growth at a concentration of 2.4 μM for the S2-013 cells (91%) and for A549 cells (70%) comparared to the free calcitriol results. At this concentration the inhibitory effect on nontumor cells (hTERT-HPNE) decreased to 65%. This study highlights the ability of PLGA nanoparticles to deliver vitamin D3 into cancer cells, with major effects regarding cancer cell cycle arrest and major changes in the cell morphological features. |
| first_indexed | 2024-12-21T18:49:33Z |
| format | Article |
| id | doaj.art-c8e5d9788bf249468053bca33e8c4f6e |
| institution | Directory Open Access Journal |
| issn | 2190-4286 |
| language | English |
| last_indexed | 2024-12-21T18:49:33Z |
| publishDate | 2015-06-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj.art-c8e5d9788bf249468053bca33e8c4f6e2022-12-21T18:53:48ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862015-06-01611306131810.3762/bjnano.6.1352190-4286-6-135PLGA nanoparticles as a platform for vitamin D-based cancer therapyMaria J. Ramalho0Joana A. Loureiro1Bárbara Gomes2Manuela F. Frasco3Manuel A. N. Coelho4M. Carmo Pereira5LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalPoly(lactic-co-glycolic acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-HPNE, and the lung cancer cell line A549. Encapsulated calcitriol retained its biological activity, reducing the cell growth. Cytotoxicity assays demonstrated that encapsulation of calcitriol enhanced its inhibitory effect on cell growth at a concentration of 2.4 μM for the S2-013 cells (91%) and for A549 cells (70%) comparared to the free calcitriol results. At this concentration the inhibitory effect on nontumor cells (hTERT-HPNE) decreased to 65%. This study highlights the ability of PLGA nanoparticles to deliver vitamin D3 into cancer cells, with major effects regarding cancer cell cycle arrest and major changes in the cell morphological features.https://doi.org/10.3762/bjnano.6.1351α,25-dihydroxyvitamin D3calcitriolcancer therapydrug deliverypoly(lactic-co-glycolic acid) |
| spellingShingle | Maria J. Ramalho Joana A. Loureiro Bárbara Gomes Manuela F. Frasco Manuel A. N. Coelho M. Carmo Pereira PLGA nanoparticles as a platform for vitamin D-based cancer therapy Beilstein Journal of Nanotechnology 1α,25-dihydroxyvitamin D3 calcitriol cancer therapy drug delivery poly(lactic-co-glycolic acid) |
| title | PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
| title_full | PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
| title_fullStr | PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
| title_full_unstemmed | PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
| title_short | PLGA nanoparticles as a platform for vitamin D-based cancer therapy |
| title_sort | plga nanoparticles as a platform for vitamin d based cancer therapy |
| topic | 1α,25-dihydroxyvitamin D3 calcitriol cancer therapy drug delivery poly(lactic-co-glycolic acid) |
| url | https://doi.org/10.3762/bjnano.6.135 |
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