Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review
Molecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors which...
| मुख्य लेखकों: | , , , , , , , |
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| स्वरूप: | लेख |
| भाषा: | English |
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Iranian Society of Pathology
2021-01-01
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| श्रृंखला: | Iranian Journal of Pathology |
| विषय: | |
| ऑनलाइन पहुंच: | https://ijp.iranpath.org/article_46859_099d228371f5c1f9ef59b863c08fb831.pdf |
| _version_ | 1831756042776608768 |
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| author | Maryam Anvari Atoosa Gharib Maryam Abolhasani Aileen Azari-Yaam Farzaneh Gharalari Moeinadin Safavi Ali Zare-Mirzaie Mohammad Vasei |
| author_facet | Maryam Anvari Atoosa Gharib Maryam Abolhasani Aileen Azari-Yaam Farzaneh Gharalari Moeinadin Safavi Ali Zare-Mirzaie Mohammad Vasei |
| author_sort | Maryam Anvari |
| collection | DOAJ |
| description | Molecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors which take place in molecular laboratories. Certain practices in specimen collection, transportation, and storage can affect the integrity of nucleic acids before analysis. Applying best practices in these steps, helps to minimize those errors and leads to better decisions in patient diagnosis and treatment. Widely acceptable recommendations, which are for optimal molecular assays associated with pre-analytic variables, are limited. In this article, we have reviewed most of the important issues in sample handling from bed to bench before starting molecular tests, which can be used in diagnostic as well as research laboratories. We have addressed the most important pre-analytical points in performing molecular analysis in fixed and unfixed solid tissues, whole blood, serum, plasma, as well as most of the body fluids including urine, fecal and bronchial samples, as well as prenatal diagnosis samples. |
| first_indexed | 2024-12-22T00:05:34Z |
| format | Article |
| id | doaj.art-c8e5f26c760e47c097f078ae3ea72b10 |
| institution | Directory Open Access Journal |
| issn | 1735-5303 2345-3656 |
| language | English |
| last_indexed | 2024-12-22T00:05:34Z |
| publishDate | 2021-01-01 |
| publisher | Iranian Society of Pathology |
| record_format | Article |
| series | Iranian Journal of Pathology |
| spelling | doaj.art-c8e5f26c760e47c097f078ae3ea72b102022-12-21T18:45:34ZengIranian Society of PathologyIranian Journal of Pathology1735-53032345-36562021-01-0116111910.30699/ijp.2020.124315.235746859Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise ReviewMaryam Anvari0Atoosa Gharib1Maryam Abolhasani2Aileen Azari-Yaam3Farzaneh Gharalari4Moeinadin Safavi5Ali Zare-Mirzaie6Mohammad Vasei7Molecular Pathology and Cytogenetics Division, Pathology Department, Children's Medical Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathology, Modarres Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IranOncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran; Hasheminejad Kidney Center, Iran University of Medical Sciences, Tehran, IranMolecular Pathology and Cytogenetics Division, Pathology Department, Children's Medical Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathology, School of Medicine, Urmia University of Medical Sciences, Urmia, IranMolecular Pathology and Cytogenetics Division, Pathology Department, Children's Medical Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, IranCell-based Therapies Research Center, Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IranMolecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors which take place in molecular laboratories. Certain practices in specimen collection, transportation, and storage can affect the integrity of nucleic acids before analysis. Applying best practices in these steps, helps to minimize those errors and leads to better decisions in patient diagnosis and treatment. Widely acceptable recommendations, which are for optimal molecular assays associated with pre-analytic variables, are limited. In this article, we have reviewed most of the important issues in sample handling from bed to bench before starting molecular tests, which can be used in diagnostic as well as research laboratories. We have addressed the most important pre-analytical points in performing molecular analysis in fixed and unfixed solid tissues, whole blood, serum, plasma, as well as most of the body fluids including urine, fecal and bronchial samples, as well as prenatal diagnosis samples.https://ijp.iranpath.org/article_46859_099d228371f5c1f9ef59b863c08fb831.pdfbody fluidsformaldehydenucleic acidspre-analytical phase |
| spellingShingle | Maryam Anvari Atoosa Gharib Maryam Abolhasani Aileen Azari-Yaam Farzaneh Gharalari Moeinadin Safavi Ali Zare-Mirzaie Mohammad Vasei Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review Iranian Journal of Pathology body fluids formaldehyde nucleic acids pre-analytical phase |
| title | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
| title_full | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
| title_fullStr | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
| title_full_unstemmed | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
| title_short | Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review |
| title_sort | pre analytical practices in the molecular diagnostic tests a concise review |
| topic | body fluids formaldehyde nucleic acids pre-analytical phase |
| url | https://ijp.iranpath.org/article_46859_099d228371f5c1f9ef59b863c08fb831.pdf |
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