Genotyping of Escherichia Coli Isolated From Clinical and Hospitals Environment
Pathogenic Escherichia coli (E. coli) strains are known to cause intestinal and extraintestinal infections in human. A wide variety of infectious diseases could be caused by E. coli strains, including urinary tract infection, septicemia, newborn meningitis, central nervous system and respiratory s...
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| Format: | Article |
| Language: | English |
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University of Thi-Qar
2019-05-01
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| Series: | مجلة علوم ذي قار |
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| Online Access: | https://www.jsci.utq.edu.iq/index.php/main/article/view/141 |
| Summary: | Pathogenic Escherichia coli (E. coli) strains are known to cause intestinal and extraintestinal infections in human.
A wide variety of infectious diseases could be caused by E. coli strains, including urinary tract infection, septicemia,
newborn meningitis, central nervous system and respiratory system infections.Several bacterial agents can cause
diarrheal and urinary tract infections, among these E.coli strains are detected as an important cause of morbidity and
mortality of diarrhoea and UTI throughout the world.A total of 318 samples were collected during the period from
September 2012 to march 2013 .All specimens were screened for the presence of E. coli by cultured on MacConkey
agar and Eosin-methylene blue agar then identified by biochemical tests and confirmed by API 20E system which
revealed that:90/318(28.33%) gave positive growth for E.coli )15 (16.63%) environmental isolates and 75(83.33%)
clinical isolates( as fallowing:1/42(2.38%)was obtained from burns,7/25(28%)from High vaginal swabs ,11/41
(26.82%) from Wounds infections,18/33(54.54%)from Diarrheal infections and38/67(56.71 %)from Urinary tract
infections, while 15/110 (13.63%) from environmental isolates, The isolated were subjected to molecular identification
as E.coli by used the intergenic spacer region primer(ITS). The confirmed isolates were examined to detect the
phylogenetic group based on triplex PCR by using a combination of two genes (chuA and yjaA) and an anonymous
DNA fragment.Universal primers (ITS) indicated that all isolates 90(100%) gave positive results.The phylogenetic
analysis revealed that 90 E.coli isolates belonged to three phylogenetic groups A &D 36(40%)for each, while B2 18
(20%) and no one isolates belong to B1 phylogenetic types .Out of the 16 of antibiotics used, 87(92.22%) isolates were
multidrug resistance.All isolates have been found resistant to at least one ?-lactam Antibiotics.
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| ISSN: | 1991-8690 2709-0256 |