Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni
From the commercial extract of the leaves of Stevia rebaudiana Bertoni, a minor steviol glycoside, 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2-O-(3-O-β-D-glucopyranosyl-α-L-rhamnopyranosyl)-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-09-01
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| Series: | Biomolecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2218-273X/3/4/733 |
| _version_ | 1818766345461825536 |
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| author | Guohong Mao Venkata Sai Prakash Chaturvedula Oliver Yu Steven Chen |
| author_facet | Guohong Mao Venkata Sai Prakash Chaturvedula Oliver Yu Steven Chen |
| author_sort | Guohong Mao |
| collection | DOAJ |
| description | From the commercial extract of the leaves of Stevia rebaudiana Bertoni, a minor steviol glycoside, 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2-O-(3-O-β-D-glucopyranosyl-α-L-rhamnopyranosyl)-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester] (1); also known as rebaudioside O having seven sugar units has been isolated. Its structural characterization has been achieved by the extensive 1D (1H and 13C), and 2D NMR (COSY, HMQC, HMBC) as well as mass spectral data. Further, hydrolysis studies were performed on rebaudioside O using acid and enzymatic methods to identify aglycone and sugar residues in its structure as well as their configurations. |
| first_indexed | 2024-12-18T08:32:31Z |
| format | Article |
| id | doaj.art-c8e6d7e8bab445d1b4cf22e168358adb |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-18T08:32:31Z |
| publishDate | 2013-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-c8e6d7e8bab445d1b4cf22e168358adb2022-12-21T21:14:25ZengMDPI AGBiomolecules2218-273X2013-09-013473374010.3390/biom3040733Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana BertoniGuohong MaoVenkata Sai Prakash ChaturvedulaOliver YuSteven ChenFrom the commercial extract of the leaves of Stevia rebaudiana Bertoni, a minor steviol glycoside, 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2-O-(3-O-β-D-glucopyranosyl-α-L-rhamnopyranosyl)-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester] (1); also known as rebaudioside O having seven sugar units has been isolated. Its structural characterization has been achieved by the extensive 1D (1H and 13C), and 2D NMR (COSY, HMQC, HMBC) as well as mass spectral data. Further, hydrolysis studies were performed on rebaudioside O using acid and enzymatic methods to identify aglycone and sugar residues in its structure as well as their configurations.http://www.mdpi.com/2218-273X/3/4/733Stevia rebaudianaditerpene glycosideisolationstructure elucidationspectral datahydrolysis studies |
| spellingShingle | Guohong Mao Venkata Sai Prakash Chaturvedula Oliver Yu Steven Chen Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni Biomolecules Stevia rebaudiana diterpene glycoside isolation structure elucidation spectral data hydrolysis studies |
| title | Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni |
| title_full | Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni |
| title_fullStr | Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni |
| title_full_unstemmed | Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni |
| title_short | Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni |
| title_sort | isolation nmr spectral analysis and hydrolysis studies of a hepta pyranosyl diterpene glycoside from stevia rebaudiana bertoni |
| topic | Stevia rebaudiana diterpene glycoside isolation structure elucidation spectral data hydrolysis studies |
| url | http://www.mdpi.com/2218-273X/3/4/733 |
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