Protective Effects of <i>Lactobacillus gasseri</i> against High-Cholesterol Diet-Induced Fatty Liver and Regulation of Host Gene Expression Profiles
Fatty liver is one of the most pervasive liver diseases worldwide. Probiotics play an important role in the progression of liver disease, but their effects on host regulation are poorly understood. This study investigated the protective effects of lactobacillus gasseri (<i>L. gasseri</i>...
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2053 |
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| author | Tianhua He Nikita Lykov Xu Luo Huiling Wang Zhanxiang Du Ziyi Chen Shitian Chen Lin Zhu Ye Zhao Chimeng Tzeng |
| author_facet | Tianhua He Nikita Lykov Xu Luo Huiling Wang Zhanxiang Du Ziyi Chen Shitian Chen Lin Zhu Ye Zhao Chimeng Tzeng |
| author_sort | Tianhua He |
| collection | DOAJ |
| description | Fatty liver is one of the most pervasive liver diseases worldwide. Probiotics play an important role in the progression of liver disease, but their effects on host regulation are poorly understood. This study investigated the protective effects of lactobacillus gasseri (<i>L. gasseri</i>) against high-cholesterol diet (HCD)-induced fatty liver injury using a zebrafish larvae model. Liver pathology, lipid accumulation, oxidative stress and hepatic inflammation were evaluated to demonstrate the changes in a spectrum of hepatic injury. Moreover, multiple indexes on host gene expression profiles were comprehensively characterized by RNA screening. The results showed that treatment with <i>L. gasseri</i> ameliorated HCD-induced morphological and histological alterations, lipid regulations, oxidative stress and macrophage aggregation in the liver of zebrafish larvae. Furthermore, the enrichment of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed that the core pathways of <i>L. gasseri</i> regulation were interleukin-17 (IL-17) signaling, phosphoinositide 3-kinase (PI3K)-AKT signaling pathway, the regulation of lipolysis and adipocytes and fatty acid elongation and estrogen signaling. The genes at key junction nodes, hsp90aa1.1, kyat3, hsd17b7, irs2a, myl9b, ptgs2b, cdk21 and papss2a were significantly regulated by <i>L. gasseri</i> administration. To conclude, the current research extends our understanding of the protective effects of <i>L. gasseri</i> against fatty liver and provides potential therapeutic options for fatty liver treatment. |
| first_indexed | 2024-03-11T09:43:12Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:43:12Z |
| publishDate | 2023-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c8e7807320d248f68ee1c5fdec9e15812023-11-16T16:51:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243205310.3390/ijms24032053Protective Effects of <i>Lactobacillus gasseri</i> against High-Cholesterol Diet-Induced Fatty Liver and Regulation of Host Gene Expression ProfilesTianhua He0Nikita Lykov1Xu Luo2Huiling Wang3Zhanxiang Du4Ziyi Chen5Shitian Chen6Lin Zhu7Ye Zhao8Chimeng Tzeng9School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, ChinaFatty liver is one of the most pervasive liver diseases worldwide. Probiotics play an important role in the progression of liver disease, but their effects on host regulation are poorly understood. This study investigated the protective effects of lactobacillus gasseri (<i>L. gasseri</i>) against high-cholesterol diet (HCD)-induced fatty liver injury using a zebrafish larvae model. Liver pathology, lipid accumulation, oxidative stress and hepatic inflammation were evaluated to demonstrate the changes in a spectrum of hepatic injury. Moreover, multiple indexes on host gene expression profiles were comprehensively characterized by RNA screening. The results showed that treatment with <i>L. gasseri</i> ameliorated HCD-induced morphological and histological alterations, lipid regulations, oxidative stress and macrophage aggregation in the liver of zebrafish larvae. Furthermore, the enrichment of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed that the core pathways of <i>L. gasseri</i> regulation were interleukin-17 (IL-17) signaling, phosphoinositide 3-kinase (PI3K)-AKT signaling pathway, the regulation of lipolysis and adipocytes and fatty acid elongation and estrogen signaling. The genes at key junction nodes, hsp90aa1.1, kyat3, hsd17b7, irs2a, myl9b, ptgs2b, cdk21 and papss2a were significantly regulated by <i>L. gasseri</i> administration. To conclude, the current research extends our understanding of the protective effects of <i>L. gasseri</i> against fatty liver and provides potential therapeutic options for fatty liver treatment.https://www.mdpi.com/1422-0067/24/3/2053fatty liver<i>Lactobacillus gasseri</i>high cholesterol dietzebrafishIL-17 |
| spellingShingle | Tianhua He Nikita Lykov Xu Luo Huiling Wang Zhanxiang Du Ziyi Chen Shitian Chen Lin Zhu Ye Zhao Chimeng Tzeng Protective Effects of <i>Lactobacillus gasseri</i> against High-Cholesterol Diet-Induced Fatty Liver and Regulation of Host Gene Expression Profiles International Journal of Molecular Sciences fatty liver <i>Lactobacillus gasseri</i> high cholesterol diet zebrafish IL-17 |
| title | Protective Effects of <i>Lactobacillus gasseri</i> against High-Cholesterol Diet-Induced Fatty Liver and Regulation of Host Gene Expression Profiles |
| title_full | Protective Effects of <i>Lactobacillus gasseri</i> against High-Cholesterol Diet-Induced Fatty Liver and Regulation of Host Gene Expression Profiles |
| title_fullStr | Protective Effects of <i>Lactobacillus gasseri</i> against High-Cholesterol Diet-Induced Fatty Liver and Regulation of Host Gene Expression Profiles |
| title_full_unstemmed | Protective Effects of <i>Lactobacillus gasseri</i> against High-Cholesterol Diet-Induced Fatty Liver and Regulation of Host Gene Expression Profiles |
| title_short | Protective Effects of <i>Lactobacillus gasseri</i> against High-Cholesterol Diet-Induced Fatty Liver and Regulation of Host Gene Expression Profiles |
| title_sort | protective effects of i lactobacillus gasseri i against high cholesterol diet induced fatty liver and regulation of host gene expression profiles |
| topic | fatty liver <i>Lactobacillus gasseri</i> high cholesterol diet zebrafish IL-17 |
| url | https://www.mdpi.com/1422-0067/24/3/2053 |
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