Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster.
In Drosophila melanogaster, specification of wing vein cells and sensory organ precursor (SOP) cells, which later give rise to a bristle, requires EGFR signaling. Here, we show that Pumilio (Pum), an RNA-binding translational repressor, negatively regulates EGFR signaling in wing vein and bristle de...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3326002?pdf=render |
| _version_ | 1828483875661152256 |
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| author | Sung Yun Kim Ji Young Kim Sumira Malik Wonseok Son Ki-Sun Kwon Changsoo Kim |
| author_facet | Sung Yun Kim Ji Young Kim Sumira Malik Wonseok Son Ki-Sun Kwon Changsoo Kim |
| author_sort | Sung Yun Kim |
| collection | DOAJ |
| description | In Drosophila melanogaster, specification of wing vein cells and sensory organ precursor (SOP) cells, which later give rise to a bristle, requires EGFR signaling. Here, we show that Pumilio (Pum), an RNA-binding translational repressor, negatively regulates EGFR signaling in wing vein and bristle development. We observed that loss of Pum function yielded extra wing veins and additional bristles. Conversely, overexpression of Pum eliminated wing veins and bristles. Heterozygotes for Pum produced no phenotype on their own, but greatly enhanced phenotypes caused by the enhancement of EGFR signaling. Conversely, over-expression of Pum suppressed the effects of ectopic EGFR signaling. Components of the EGFR signaling pathway are encoded by mRNAs that have Nanos Response Element (NRE)-like sequences in their 3'UTRs; NREs are known to bind Pum to confer regulation in other mRNAs. We show that these NRE-like sequences bind Pum and confer repression on a luciferase reporter in heterologous cells. Taken together, our evidence suggests that Pum functions as a negative regulator of EGFR signaling by directly targeting components of the pathway in Drosophila. |
| first_indexed | 2024-12-11T08:45:35Z |
| format | Article |
| id | doaj.art-c8e96de8698a4d44808c5c7926b5601e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:45:35Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c8e96de8698a4d44808c5c7926b5601e2022-12-22T01:14:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3401610.1371/journal.pone.0034016Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster.Sung Yun KimJi Young KimSumira MalikWonseok SonKi-Sun KwonChangsoo KimIn Drosophila melanogaster, specification of wing vein cells and sensory organ precursor (SOP) cells, which later give rise to a bristle, requires EGFR signaling. Here, we show that Pumilio (Pum), an RNA-binding translational repressor, negatively regulates EGFR signaling in wing vein and bristle development. We observed that loss of Pum function yielded extra wing veins and additional bristles. Conversely, overexpression of Pum eliminated wing veins and bristles. Heterozygotes for Pum produced no phenotype on their own, but greatly enhanced phenotypes caused by the enhancement of EGFR signaling. Conversely, over-expression of Pum suppressed the effects of ectopic EGFR signaling. Components of the EGFR signaling pathway are encoded by mRNAs that have Nanos Response Element (NRE)-like sequences in their 3'UTRs; NREs are known to bind Pum to confer regulation in other mRNAs. We show that these NRE-like sequences bind Pum and confer repression on a luciferase reporter in heterologous cells. Taken together, our evidence suggests that Pum functions as a negative regulator of EGFR signaling by directly targeting components of the pathway in Drosophila.http://europepmc.org/articles/PMC3326002?pdf=render |
| spellingShingle | Sung Yun Kim Ji Young Kim Sumira Malik Wonseok Son Ki-Sun Kwon Changsoo Kim Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster. PLoS ONE |
| title | Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster. |
| title_full | Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster. |
| title_fullStr | Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster. |
| title_full_unstemmed | Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster. |
| title_short | Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster. |
| title_sort | negative regulation of egfr mapk pathway by pumilio in drosophila melanogaster |
| url | http://europepmc.org/articles/PMC3326002?pdf=render |
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