Immunohistochemical Analysis of Vimentin and Zonula Occludens-1 in Placentas of Patients with PPROM
Background: We aimed to investigate the immunohistochemical staining of vimentin and zonula occludens-1 (ZO-1) expression in the placentas of pregnant women with preterm premature rupture of membranes (PPROM). Methods: Placentas of 25 healthy and 25 women with PPROM were fixed in 10% formaldehyde so...
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| Format: | Article |
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IMR Press
2024-01-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/51/1/10.31083/j.ceog5101018 |
| _version_ | 1797338510710013952 |
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| author | Fuat Zaman Fırat Aşır Işılay Sezen Ermiş Mehmet Cudi Tuncer Engin Deveci Süleyman Cemil Oğlak |
| author_facet | Fuat Zaman Fırat Aşır Işılay Sezen Ermiş Mehmet Cudi Tuncer Engin Deveci Süleyman Cemil Oğlak |
| author_sort | Fuat Zaman |
| collection | DOAJ |
| description | Background: We aimed to investigate the immunohistochemical staining of vimentin and zonula occludens-1 (ZO-1) expression in the placentas of pregnant women with preterm premature rupture of membranes (PPROM). Methods: Placentas of 25 healthy and 25 women with PPROM were fixed in 10% formaldehyde solution and further processed for paraffin wax tissue embedding. Demographic properties of patients were recorded. Placentas were histologically stained with hematoxylin-eosin and vimentin and ZO-1 expression immunostaining. Results: Vimentin expression was high in the decidual cells, fibroblasts, and connective tissue fibers in control group. Compared to control group, vimentin expression was decreased in the placental structures of PPROM group, where fetal membranes were degenerated and histologically irregular. Similar to vimentin expression, ZO-1 expression was also high in placental components of control group such as chorioamniotic membrane and amniotic epithelium. The PPROM group showed lower expression of ZO-1 expression in placental structures than in that of control. ZO-1 expression was significantly lowered in regions where fetal membrane integrity was weakened and lost. Conclusions: We suggest that ZO-1 and vimentin expression may show alteration in etiology premature rupture of membrane. |
| first_indexed | 2024-03-08T09:32:18Z |
| format | Article |
| id | doaj.art-c8ed72e42aab44288274f157e7efea5e |
| institution | Directory Open Access Journal |
| issn | 0390-6663 |
| language | English |
| last_indexed | 2024-03-08T09:32:18Z |
| publishDate | 2024-01-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj.art-c8ed72e42aab44288274f157e7efea5e2024-01-31T01:12:31ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632024-01-015111810.31083/j.ceog5101018S0390-6663(23)02259-5Immunohistochemical Analysis of Vimentin and Zonula Occludens-1 in Placentas of Patients with PPROMFuat Zaman0Fırat Aşır1Işılay Sezen Ermiş2Mehmet Cudi Tuncer3Engin Deveci4Süleyman Cemil Oğlak5Department of Obstetrics and Gynecology, Diyarlife Hospital, 21100 Diyarbakır, TurkeyDepartment of Histology and Embryology, Medical Faculty, Dicle University, 21280 Diyarbakır, TurkeyDepartment of Gynecology and Obstetrics, Medical Faculty, Harran University, 63300 Şanlıurfa, TurkeyDepartment of Anatomy, Medical Faculty, Dicle University, 21280 Diyarbakır, TurkeyDepartment of Histology and Embryology, Medical Faculty, Dicle University, 21280 Diyarbakır, TurkeyDivision of Gynecology and Obstetrics, Diyarbakır Gazi Yaşargil Training and Research Hospital, 21010 Diyarbakır, TurkeyBackground: We aimed to investigate the immunohistochemical staining of vimentin and zonula occludens-1 (ZO-1) expression in the placentas of pregnant women with preterm premature rupture of membranes (PPROM). Methods: Placentas of 25 healthy and 25 women with PPROM were fixed in 10% formaldehyde solution and further processed for paraffin wax tissue embedding. Demographic properties of patients were recorded. Placentas were histologically stained with hematoxylin-eosin and vimentin and ZO-1 expression immunostaining. Results: Vimentin expression was high in the decidual cells, fibroblasts, and connective tissue fibers in control group. Compared to control group, vimentin expression was decreased in the placental structures of PPROM group, where fetal membranes were degenerated and histologically irregular. Similar to vimentin expression, ZO-1 expression was also high in placental components of control group such as chorioamniotic membrane and amniotic epithelium. The PPROM group showed lower expression of ZO-1 expression in placental structures than in that of control. ZO-1 expression was significantly lowered in regions where fetal membrane integrity was weakened and lost. Conclusions: We suggest that ZO-1 and vimentin expression may show alteration in etiology premature rupture of membrane.https://www.imrpress.com/journal/CEOG/51/1/10.31083/j.ceog5101018promamniotic membranejunctionhistopathology |
| spellingShingle | Fuat Zaman Fırat Aşır Işılay Sezen Ermiş Mehmet Cudi Tuncer Engin Deveci Süleyman Cemil Oğlak Immunohistochemical Analysis of Vimentin and Zonula Occludens-1 in Placentas of Patients with PPROM Clinical and Experimental Obstetrics & Gynecology prom amniotic membrane junction histopathology |
| title | Immunohistochemical Analysis of Vimentin and Zonula Occludens-1 in Placentas of Patients with PPROM |
| title_full | Immunohistochemical Analysis of Vimentin and Zonula Occludens-1 in Placentas of Patients with PPROM |
| title_fullStr | Immunohistochemical Analysis of Vimentin and Zonula Occludens-1 in Placentas of Patients with PPROM |
| title_full_unstemmed | Immunohistochemical Analysis of Vimentin and Zonula Occludens-1 in Placentas of Patients with PPROM |
| title_short | Immunohistochemical Analysis of Vimentin and Zonula Occludens-1 in Placentas of Patients with PPROM |
| title_sort | immunohistochemical analysis of vimentin and zonula occludens 1 in placentas of patients with pprom |
| topic | prom amniotic membrane junction histopathology |
| url | https://www.imrpress.com/journal/CEOG/51/1/10.31083/j.ceog5101018 |
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