Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation
Maltol (3-hydroxy-2-methyl-4-pyrone) is used widely as a food and cosmetic supplement, and it has antioxidant and anti-inflammatory activities. Inflammasome causes the maturation and secretion of interleukin (IL)-1β and -18 through the activation of caspase-1 (Casp1), which contributes to various in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1923 |
| _version_ | 1827652038872268800 |
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| author | Huijeong Ahn Gilyoung Lee Byung-Cheol Han Seung-Ho Lee Geun-Shik Lee |
| author_facet | Huijeong Ahn Gilyoung Lee Byung-Cheol Han Seung-Ho Lee Geun-Shik Lee |
| author_sort | Huijeong Ahn |
| collection | DOAJ |
| description | Maltol (3-hydroxy-2-methyl-4-pyrone) is used widely as a food and cosmetic supplement, and it has antioxidant and anti-inflammatory activities. Inflammasome causes the maturation and secretion of interleukin (IL)-1β and -18 through the activation of caspase-1 (Casp1), which contributes to various inflammatory diseases. This study examined the effects of maltol on the inflammasome activation in macrophages and mice. Lipopolysaccharide (LPS)-primed macrophages were treated with a trigger of NLRP3, NLRC4, AIM2, or non-canonical (NC) inflammasomes in the presence of maltol. The secretion of IL-1β and IL-18 and the cleavage of Casp1 were analyzed as indices of inflammasome activation. Mice were injected with LPS and an NLRP3 trigger with or without maltol, and the peritoneal IL-1β secretions were observed. The effects of maltol on reactive oxygen species (ROS) production and Casp1 activity were analyzed to determine the mechanism. Maltol inhibited the activation of NLRP3 and NC inflammasomes, but it did not alter the other inflammasomes. Maltol also attenuated IL-1β secretion resulting from the inflammasome activation in mice. The anti-inflammatory mechanism of maltol was revealed by the inhibition of ROS production and Casp1 activity. Maltol is suggested to be promising as a anti-inflammasome molecule. |
| first_indexed | 2024-03-09T20:49:54Z |
| format | Article |
| id | doaj.art-c8eef79bdede44e5a5804d5062ce1f54 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:49:54Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-c8eef79bdede44e5a5804d5062ce1f542023-11-23T22:37:54ZengMDPI AGAntioxidants2076-39212022-09-011110192310.3390/antiox11101923Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome ActivationHuijeong Ahn0Gilyoung Lee1Byung-Cheol Han2Seung-Ho Lee3Geun-Shik Lee4College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, KoreaCollege of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, KoreaCollege of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, KoreaKorea Ginseng Research Institute, Korea Ginseng Corporation, Daejeon 34337, KoreaCollege of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, KoreaMaltol (3-hydroxy-2-methyl-4-pyrone) is used widely as a food and cosmetic supplement, and it has antioxidant and anti-inflammatory activities. Inflammasome causes the maturation and secretion of interleukin (IL)-1β and -18 through the activation of caspase-1 (Casp1), which contributes to various inflammatory diseases. This study examined the effects of maltol on the inflammasome activation in macrophages and mice. Lipopolysaccharide (LPS)-primed macrophages were treated with a trigger of NLRP3, NLRC4, AIM2, or non-canonical (NC) inflammasomes in the presence of maltol. The secretion of IL-1β and IL-18 and the cleavage of Casp1 were analyzed as indices of inflammasome activation. Mice were injected with LPS and an NLRP3 trigger with or without maltol, and the peritoneal IL-1β secretions were observed. The effects of maltol on reactive oxygen species (ROS) production and Casp1 activity were analyzed to determine the mechanism. Maltol inhibited the activation of NLRP3 and NC inflammasomes, but it did not alter the other inflammasomes. Maltol also attenuated IL-1β secretion resulting from the inflammasome activation in mice. The anti-inflammatory mechanism of maltol was revealed by the inhibition of ROS production and Casp1 activity. Maltol is suggested to be promising as a anti-inflammasome molecule.https://www.mdpi.com/2076-3921/11/10/1923maltolinflammasomemacrophagesinterleukin-1beta |
| spellingShingle | Huijeong Ahn Gilyoung Lee Byung-Cheol Han Seung-Ho Lee Geun-Shik Lee Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation Antioxidants maltol inflammasome macrophages interleukin-1beta |
| title | Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation |
| title_full | Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation |
| title_fullStr | Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation |
| title_full_unstemmed | Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation |
| title_short | Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation |
| title_sort | maltol a natural flavor enhancer inhibits nlrp3 and non canonical inflammasome activation |
| topic | maltol inflammasome macrophages interleukin-1beta |
| url | https://www.mdpi.com/2076-3921/11/10/1923 |
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