mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of Brazil
ABSTRACT: This study detected the presence and distribution of mecA in Staphylococcus spp. in the dairy production environment at farm level in Brazil. We analyzed 335 samples of mastitis cow milk, 15 samples of nostrils and hand swabs from milkers, 14 teat cup swabs, and 9 milking buckets swabs. In...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2021-09-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000300451&tlng=en |
| _version_ | 1798014515688767488 |
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| author | José Givanildo da Silva Anderson Carlos Camargo Renata Pimentel Bandeira de Melo Breno Bezerra Aragão Junior Mário Baltazar de Oliveira Maria José de Sena Luís Augusto Nero Rinaldo Aparecido Mota |
| author_facet | José Givanildo da Silva Anderson Carlos Camargo Renata Pimentel Bandeira de Melo Breno Bezerra Aragão Junior Mário Baltazar de Oliveira Maria José de Sena Luís Augusto Nero Rinaldo Aparecido Mota |
| author_sort | José Givanildo da Silva |
| collection | DOAJ |
| description | ABSTRACT: This study detected the presence and distribution of mecA in Staphylococcus spp. in the dairy production environment at farm level in Brazil. We analyzed 335 samples of mastitis cow milk, 15 samples of nostrils and hand swabs from milkers, 14 teat cup swabs, and 9 milking buckets swabs. Initially, the samples were subjected to microbiological analysis to detect Staphylococcus spp. and then S. aureus and mecA positive isolates were identified by PCR. All S. aureus isolates carrying the mecA genes were subjected to DNA macro-restriction analysis by Pulsed-Field Gel Electrophoresis (PFGE). The mecA gene was detected in 6/335 (1.78%) of mastitis cow milk, 5/15 (33.3%), and 5/15 (33.3%) of nostrils and hand swab, and 4/14 (28.5%) of the teat cup isolates. MRSA genotyping was performed by PFGE, a total of seven pulsotypes were grouped in two clusters. This study identified the occurrence and spread of MRSA at dairy environment of farms, and also the existence of distinct genetic profiles between isolates. |
| first_indexed | 2024-04-11T15:18:28Z |
| format | Article |
| id | doaj.art-c8ef182f7d0045ccaa0be239bf4e8ada |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-04-11T15:18:28Z |
| publishDate | 2021-09-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-c8ef182f7d0045ccaa0be239bf4e8ada2022-12-22T04:16:25ZengUniversidade Federal de Santa MariaCiência Rural1678-45962021-09-0152310.1590/0103-8478cr20210008mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of BrazilJosé Givanildo da Silvahttps://orcid.org/0000-0001-5565-7980Anderson Carlos CamargoRenata Pimentel Bandeira de Melohttps://orcid.org/0000-0001-7162-2745Breno Bezerra Aragãohttps://orcid.org/0000-0002-9550-3692Junior Mário Baltazar de OliveiraMaria José de Senahttps://orcid.org/0000-0002-7845-7452Luís Augusto Nerohttps://orcid.org/0000-0002-4954-5824Rinaldo Aparecido Motahttps://orcid.org/0000-0002-2844-5509ABSTRACT: This study detected the presence and distribution of mecA in Staphylococcus spp. in the dairy production environment at farm level in Brazil. We analyzed 335 samples of mastitis cow milk, 15 samples of nostrils and hand swabs from milkers, 14 teat cup swabs, and 9 milking buckets swabs. Initially, the samples were subjected to microbiological analysis to detect Staphylococcus spp. and then S. aureus and mecA positive isolates were identified by PCR. All S. aureus isolates carrying the mecA genes were subjected to DNA macro-restriction analysis by Pulsed-Field Gel Electrophoresis (PFGE). The mecA gene was detected in 6/335 (1.78%) of mastitis cow milk, 5/15 (33.3%), and 5/15 (33.3%) of nostrils and hand swab, and 4/14 (28.5%) of the teat cup isolates. MRSA genotyping was performed by PFGE, a total of seven pulsotypes were grouped in two clusters. This study identified the occurrence and spread of MRSA at dairy environment of farms, and also the existence of distinct genetic profiles between isolates.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000300451&tlng=enS. aureusmecA genemultidrug resistanceanimal healthdairy production. |
| spellingShingle | José Givanildo da Silva Anderson Carlos Camargo Renata Pimentel Bandeira de Melo Breno Bezerra Aragão Junior Mário Baltazar de Oliveira Maria José de Sena Luís Augusto Nero Rinaldo Aparecido Mota mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of Brazil Ciência Rural S. aureus mecA gene multidrug resistance animal health dairy production. |
| title | mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of Brazil |
| title_full | mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of Brazil |
| title_fullStr | mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of Brazil |
| title_full_unstemmed | mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of Brazil |
| title_short | mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of Brazil |
| title_sort | meca positive staphylococcus spp in bovine mastitis milkers milking environment and the circulation of different mrsa clones at dairy cows farms in the northeast region of brazil |
| topic | S. aureus mecA gene multidrug resistance animal health dairy production. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000300451&tlng=en |
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