Effects of Ultrasound Targeted Destruction of Echogenic Nanobubbles Containing Doxorubicin in Rat Liver
Ultrasound is a widely used diagnostic medical imaging modality, and its therapeutic potential has recently been reported. This study examined whether ultrasound irradiation causes nanobubble destruction and identified the most effective frequency and duration of ultrasound for doxorubicin delivery...
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| Format: | Article |
| Language: | English |
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The Korean Surgical Ultrasound Society
2023-11-01
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| Series: | Journal of Surgical Ultrasound |
| Subjects: | |
| Online Access: | http://www.jsu.or.kr/journal/view.html?doi=10.46268/jsu.2023.10.2.32 |
| _version_ | 1797400819765608448 |
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| author | Jung Hyun Park Il Young Park |
| author_facet | Jung Hyun Park Il Young Park |
| author_sort | Jung Hyun Park |
| collection | DOAJ |
| description | Ultrasound is a widely used diagnostic medical imaging modality, and its therapeutic potential has recently been reported. This study examined whether ultrasound irradiation causes nanobubble destruction and identified the most effective frequency and duration of ultrasound for doxorubicin delivery in rat liver under ultrasound-targeted nanobubble destruction. Rats underwent an intravenous nanobubble injection via the caudal vein. An ultrasound probe was applied to the rat liver. The rats were divided into different groups based on the frequency. Fluorescence images were acquired using an IVIS® system. Laboratory tests were performed to determine the serum levels of AST (aspartate aminotransferase), ALT (alanine aminotransferase), total bilirubin, and creatinine. Under ultrasound irradiation, more nanobubbles were destroyed, and the doxorubicin uptake was increased in the rat liver (P ＜ 0.05). The 5 MHz group showed an increased fluorescence efficiency in the liver (P ＜ 0.05). Based on the duration of ultrasound irradiation, the 5, 10, and 13 MHz groups showed an increased fluorescence efficiency in the liver compared to the control group (P ＜ 0.05). There was no significant difference between the 15- and 30-minute groups. The other organs showed no significant differences compared to the control group. The groups to which ultrasound was applied showed increased serum AST and ALT levels, but the effects did not last for 30 min. Ultrasound-targeted nanobubble destruction was effective for doxorubicin delivery in the rat liver. The most effective ultrasound frequency and irradiation duration were 5 MHz and 15 min, respectively. |
| first_indexed | 2024-03-09T02:00:04Z |
| format | Article |
| id | doaj.art-c8f00ed23cc64f32914578b252f4a999 |
| institution | Directory Open Access Journal |
| issn | 2288-9140 |
| language | English |
| last_indexed | 2024-03-09T02:00:04Z |
| publishDate | 2023-11-01 |
| publisher | The Korean Surgical Ultrasound Society |
| record_format | Article |
| series | Journal of Surgical Ultrasound |
| spelling | doaj.art-c8f00ed23cc64f32914578b252f4a9992023-12-08T05:35:44ZengThe Korean Surgical Ultrasound SocietyJournal of Surgical Ultrasound2288-91402023-11-01102324110.46268/jsu.2023.10.2.32jsu.2023.10.2.32Effects of Ultrasound Targeted Destruction of Echogenic Nanobubbles Containing Doxorubicin in Rat LiverJung Hyun Park0Il Young Park1Department of Surgery, The Catholic University of Korea, Eunpyeong St. Mary’s Hospital, Seoul, KoreaThe Catholic University of Korea, College of Medicine, Seoul, KoreaUltrasound is a widely used diagnostic medical imaging modality, and its therapeutic potential has recently been reported. This study examined whether ultrasound irradiation causes nanobubble destruction and identified the most effective frequency and duration of ultrasound for doxorubicin delivery in rat liver under ultrasound-targeted nanobubble destruction. Rats underwent an intravenous nanobubble injection via the caudal vein. An ultrasound probe was applied to the rat liver. The rats were divided into different groups based on the frequency. Fluorescence images were acquired using an IVIS® system. Laboratory tests were performed to determine the serum levels of AST (aspartate aminotransferase), ALT (alanine aminotransferase), total bilirubin, and creatinine. Under ultrasound irradiation, more nanobubbles were destroyed, and the doxorubicin uptake was increased in the rat liver (P ＜ 0.05). The 5 MHz group showed an increased fluorescence efficiency in the liver (P ＜ 0.05). Based on the duration of ultrasound irradiation, the 5, 10, and 13 MHz groups showed an increased fluorescence efficiency in the liver compared to the control group (P ＜ 0.05). There was no significant difference between the 15- and 30-minute groups. The other organs showed no significant differences compared to the control group. The groups to which ultrasound was applied showed increased serum AST and ALT levels, but the effects did not last for 30 min. Ultrasound-targeted nanobubble destruction was effective for doxorubicin delivery in the rat liver. The most effective ultrasound frequency and irradiation duration were 5 MHz and 15 min, respectively.http://www.jsu.or.kr/journal/view.html?doi=10.46268/jsu.2023.10.2.32microbubbleultrasoundfrequencydurationdoxorubicin |
| spellingShingle | Jung Hyun Park Il Young Park Effects of Ultrasound Targeted Destruction of Echogenic Nanobubbles Containing Doxorubicin in Rat Liver Journal of Surgical Ultrasound microbubble ultrasound frequency duration doxorubicin |
| title | Effects of Ultrasound Targeted Destruction of Echogenic Nanobubbles Containing Doxorubicin in Rat Liver |
| title_full | Effects of Ultrasound Targeted Destruction of Echogenic Nanobubbles Containing Doxorubicin in Rat Liver |
| title_fullStr | Effects of Ultrasound Targeted Destruction of Echogenic Nanobubbles Containing Doxorubicin in Rat Liver |
| title_full_unstemmed | Effects of Ultrasound Targeted Destruction of Echogenic Nanobubbles Containing Doxorubicin in Rat Liver |
| title_short | Effects of Ultrasound Targeted Destruction of Echogenic Nanobubbles Containing Doxorubicin in Rat Liver |
| title_sort | effects of ultrasound targeted destruction of echogenic nanobubbles containing doxorubicin in rat liver |
| topic | microbubble ultrasound frequency duration doxorubicin |
| url | http://www.jsu.or.kr/journal/view.html?doi=10.46268/jsu.2023.10.2.32 |
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