PROBIT ANALYSIS FOR Cd, Pb, Cu, Zn PHYTOTOXICITY ASSESSMENT

The aim of investigation was to develop a new approach in Cd, Zn, Cu, Pb phytotoxicity assessing. This approach provided the probit analysis using. Methods included probit analysis, thin layer chromatography, statistical methods (calculation of the least significant difference, correlation analysis)...

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Main Authors: N. O. Ryzhenko, V. M. Kavetsky
Format: Article
Language:English
Published: National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry 2017-04-01
Series:Biotechnologia Acta
Subjects:
Online Access:http://biotechnology.kiev.ua/images/storage/2_2017/ryzhenko_2_2017.pdf
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author N. O. Ryzhenko
V. M. Kavetsky
author_facet N. O. Ryzhenko
V. M. Kavetsky
author_sort N. O. Ryzhenko
collection DOAJ
description The aim of investigation was to develop a new approach in Cd, Zn, Cu, Pb phytotoxicity assessing. This approach provided the probit analysis using. Methods included probit analysis, thin layer chromatography, statistical methods (calculation of the least significant difference, correlation analysis). We applied «doze-effect» model to obtain the ranking of the metals according to their phytotoxicity in spring barley field. We offered to estimate the phytotoxicity by PhLD50 index. Research results were: graphic formalization of "dose-effect" dependence and calculation of phytotoxic doses (PhLD50 and PhLD95) for Cd, Cu, Pb, Zn in polluted soil. According to PhLD50 value we conducted a comparative assessment of Cd, Cu, Pb, Zn phytotoxicity relatively to spring barley. According to PhLD50 value metals could be ranked: Cd> Cu> Pb> Zn. The most toxic metal was Cd. PhLD values, on which the estimation of metals phytotoxicity, were: Cd – 50, Cu –129, Pb –537, Zn –603 mg / kg mobile forms in turf-podzol sandy loam soil. Our findings are relevant to estimating the metal hazard and controlling the condition of the crop growth.
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spelling doaj.art-c8f1cf324ac74c0f81874b6320ea7b2d2023-08-02T00:04:39ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2017-04-01102677410.15407/biotech10.02.067PROBIT ANALYSIS FOR Cd, Pb, Cu, Zn PHYTOTOXICITY ASSESSMENTN. O. Ryzhenko0 V. M. Kavetsky11State Ecological Academy of Post-Graduate Education and Management, KyivNational University of Kyiv-Mohylanska Academy The aim of investigation was to develop a new approach in Cd, Zn, Cu, Pb phytotoxicity assessing. This approach provided the probit analysis using. Methods included probit analysis, thin layer chromatography, statistical methods (calculation of the least significant difference, correlation analysis). We applied «doze-effect» model to obtain the ranking of the metals according to their phytotoxicity in spring barley field. We offered to estimate the phytotoxicity by PhLD50 index. Research results were: graphic formalization of "dose-effect" dependence and calculation of phytotoxic doses (PhLD50 and PhLD95) for Cd, Cu, Pb, Zn in polluted soil. According to PhLD50 value we conducted a comparative assessment of Cd, Cu, Pb, Zn phytotoxicity relatively to spring barley. According to PhLD50 value metals could be ranked: Cd> Cu> Pb> Zn. The most toxic metal was Cd. PhLD values, on which the estimation of metals phytotoxicity, were: Cd – 50, Cu –129, Pb –537, Zn –603 mg / kg mobile forms in turf-podzol sandy loam soil. Our findings are relevant to estimating the metal hazard and controlling the condition of the crop growth.http://biotechnology.kiev.ua/images/storage/2_2017/ryzhenko_2_2017.pdfheavy metalsphytotoxicityprobit analysis
spellingShingle N. O. Ryzhenko
V. M. Kavetsky
PROBIT ANALYSIS FOR Cd, Pb, Cu, Zn PHYTOTOXICITY ASSESSMENT
Biotechnologia Acta
heavy metals
phytotoxicity
probit analysis
title PROBIT ANALYSIS FOR Cd, Pb, Cu, Zn PHYTOTOXICITY ASSESSMENT
title_full PROBIT ANALYSIS FOR Cd, Pb, Cu, Zn PHYTOTOXICITY ASSESSMENT
title_fullStr PROBIT ANALYSIS FOR Cd, Pb, Cu, Zn PHYTOTOXICITY ASSESSMENT
title_full_unstemmed PROBIT ANALYSIS FOR Cd, Pb, Cu, Zn PHYTOTOXICITY ASSESSMENT
title_short PROBIT ANALYSIS FOR Cd, Pb, Cu, Zn PHYTOTOXICITY ASSESSMENT
title_sort probit analysis for cd pb cu zn phytotoxicity assessment
topic heavy metals
phytotoxicity
probit analysis
url http://biotechnology.kiev.ua/images/storage/2_2017/ryzhenko_2_2017.pdf
work_keys_str_mv AT noryzhenko probitanalysisforcdpbcuznphytotoxicityassessment
AT vmkavetsky probitanalysisforcdpbcuznphytotoxicityassessment