Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies
African swine fever (ASF) is a viral disease caused by the African swine fever virus that can be highly transmitted and lethal in domestic pigs. In the absence of a vaccine, effective diagnosis is critical for minimizing the virus’s spread. In recent years, with the decline of African swine fever vi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1308753/full |
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| author | Yuanyuan Tian Yuanyuan Tian Chao Liang Chao Liang Jingming Zhou Jingming Zhou Fanglin Sun Fanglin Sun Yankai Liu Yankai Liu Yumei Chen Yumei Chen Xifang Zhu Xifang Zhu Hongliang Liu Hongliang Liu Peiyang Ding Peiyang Ding Enping Liu Enping Liu Ying Zhang Ying Zhang Sixuan Wu Sixuan Wu Aiping Wang Aiping Wang Aiping Wang |
| author_facet | Yuanyuan Tian Yuanyuan Tian Chao Liang Chao Liang Jingming Zhou Jingming Zhou Fanglin Sun Fanglin Sun Yankai Liu Yankai Liu Yumei Chen Yumei Chen Xifang Zhu Xifang Zhu Hongliang Liu Hongliang Liu Peiyang Ding Peiyang Ding Enping Liu Enping Liu Ying Zhang Ying Zhang Sixuan Wu Sixuan Wu Aiping Wang Aiping Wang Aiping Wang |
| author_sort | Yuanyuan Tian |
| collection | DOAJ |
| description | African swine fever (ASF) is a viral disease caused by the African swine fever virus that can be highly transmitted and lethal in domestic pigs. In the absence of a vaccine, effective diagnosis is critical for minimizing the virus’s spread. In recent years, with the decline of African swine fever virus (ASFV) virulence, antibody detection has become an important means of detection. ASFV nucleocapsid protein p34 is a mature hydrolytic product of pp220, which is highly conserved and has a high content in the structural protein of the virus. Prokaryotic cells were chosen to generate highly active and high-yield p34 protein, which was then used as an antigen for producing mouse monoclonal antibodies. The B-cell epitope 202QKELDKLQT210, which was highly conserved and found on the surface of the p34 protein, was first identified by an anti-p34 monoclonal antibody utilizing the peptide scanning technique and visualized in helix. This supported the viability of p34 protein detection even further. In addition, we established an indirect ELISA assay based on p34 to detect ASFV antibodies. The coincidence rate of this method with commercially available kits was shown to be 97.83%. Sensitivity analysis revealed that it could be detected in serum dilution as low as 1:6400, and there was no cross-reaction with other prevalent porcine epidemic diseases classical swine fever virus (CSFV), foot-and-mouth disease virus (FMDV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine circovirus 2 (PCV2). In summary, the established ELISA method and anti-P34 monoclonal antibody have demonstrated that the p34 protein has a promising application prospect for the detection of African swine fever antibodies. |
| first_indexed | 2024-03-08T14:28:28Z |
| format | Article |
| id | doaj.art-c8f27b20dfa6433fb7ed3b7d3cf29a13 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T14:28:28Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c8f27b20dfa6433fb7ed3b7d3cf29a132024-01-12T16:38:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-01-011410.3389/fmicb.2023.13087531308753Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodiesYuanyuan Tian0Yuanyuan Tian1Chao Liang2Chao Liang3Jingming Zhou4Jingming Zhou5Fanglin Sun6Fanglin Sun7Yankai Liu8Yankai Liu9Yumei Chen10Yumei Chen11Xifang Zhu12Xifang Zhu13Hongliang Liu14Hongliang Liu15Peiyang Ding16Peiyang Ding17Enping Liu18Enping Liu19Ying Zhang20Ying Zhang21Sixuan Wu22Sixuan Wu23Aiping Wang24Aiping Wang25Aiping Wang26Longhu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaLonghu Laboratory, Zhengzhou, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaAfrican swine fever (ASF) is a viral disease caused by the African swine fever virus that can be highly transmitted and lethal in domestic pigs. In the absence of a vaccine, effective diagnosis is critical for minimizing the virus’s spread. In recent years, with the decline of African swine fever virus (ASFV) virulence, antibody detection has become an important means of detection. ASFV nucleocapsid protein p34 is a mature hydrolytic product of pp220, which is highly conserved and has a high content in the structural protein of the virus. Prokaryotic cells were chosen to generate highly active and high-yield p34 protein, which was then used as an antigen for producing mouse monoclonal antibodies. The B-cell epitope 202QKELDKLQT210, which was highly conserved and found on the surface of the p34 protein, was first identified by an anti-p34 monoclonal antibody utilizing the peptide scanning technique and visualized in helix. This supported the viability of p34 protein detection even further. In addition, we established an indirect ELISA assay based on p34 to detect ASFV antibodies. The coincidence rate of this method with commercially available kits was shown to be 97.83%. Sensitivity analysis revealed that it could be detected in serum dilution as low as 1:6400, and there was no cross-reaction with other prevalent porcine epidemic diseases classical swine fever virus (CSFV), foot-and-mouth disease virus (FMDV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine circovirus 2 (PCV2). In summary, the established ELISA method and anti-P34 monoclonal antibody have demonstrated that the p34 protein has a promising application prospect for the detection of African swine fever antibodies.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1308753/fullAfrican swine fever virusp34 proteinmonoclonal antibodyB-cell epitopeantibody detectionELISA |
| spellingShingle | Yuanyuan Tian Yuanyuan Tian Chao Liang Chao Liang Jingming Zhou Jingming Zhou Fanglin Sun Fanglin Sun Yankai Liu Yankai Liu Yumei Chen Yumei Chen Xifang Zhu Xifang Zhu Hongliang Liu Hongliang Liu Peiyang Ding Peiyang Ding Enping Liu Enping Liu Ying Zhang Ying Zhang Sixuan Wu Sixuan Wu Aiping Wang Aiping Wang Aiping Wang Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies Frontiers in Microbiology African swine fever virus p34 protein monoclonal antibody B-cell epitope antibody detection ELISA |
| title | Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies |
| title_full | Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies |
| title_fullStr | Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies |
| title_full_unstemmed | Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies |
| title_short | Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies |
| title_sort | identification of a novel b cell epitope of the african swine fever virus p34 protein and development of an indirect elisa for the detection of serum antibodies |
| topic | African swine fever virus p34 protein monoclonal antibody B-cell epitope antibody detection ELISA |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1308753/full |
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