Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition
The aim of the study was to define ultrastructural changes in the ovarian cyclic corpus luteum (CL) of dairy cows with different body condition score (BCS, a 5-point scale). The ovaries were recovered from slaughtered Holstein cows of BCS1 (emaciation; n= 4), BCS2 (tendency to emaciation; n=4), BCS3...
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| Format: | Article |
| Language: | English |
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Sciendo
2016-06-01
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| Series: | Acta Veterinaria |
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| Online Access: | https://doi.org/10.1515/acve-2016-0021 |
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| author | Pivko Juraj Makarevich V. Alexander Kubovičova Elena Rafay Jan Chrenek Peter |
| author_facet | Pivko Juraj Makarevich V. Alexander Kubovičova Elena Rafay Jan Chrenek Peter |
| author_sort | Pivko Juraj |
| collection | DOAJ |
| description | The aim of the study was to define ultrastructural changes in the ovarian cyclic corpus luteum (CL) of dairy cows with different body condition score (BCS, a 5-point scale). The ovaries were recovered from slaughtered Holstein cows of BCS1 (emaciation; n= 4), BCS2 (tendency to emaciation; n=4), BCS3 (average body condition; n= 4), BCS4 (tendency to fattening; n=4) and BCS5 (fattening; n=4). Average ages of cows were: 6.2, 4.1, 5.7, 5.5 and 6.36 years for BCS1, BCS2, BCS3, BCS4 and BCS5, respectively. For electron microscopy, functioning CL at the mid-luteal phase were fixed in the aldehyde solution and post-fixed in 1% OsO4 solution. For lipid visualization the samples were soaked in 1% p-phenylenediamine solution in acetone. Durcupan ultrathin sections (90 nm) were contrasted with uranyl acetate and lead citrate and viewed under Jeol transmission electron microscope. Occurrence of cell organelles was determined by counting the relative volume of individual cellular components using the modified universal test network. Luteal regression of cyclic CL was characterized by atrophy of lutein cells, condensation of organelles and cytoplasmic inclusions, increase in relative volumes of vacuoles, lysosomes, peroxisomes, autophagosomes and lipids. There were significant differences (p<0.05) among BCS1, BCS2, BCS4 and BCS5 cows in the occurrence of lipids, vacuoles, lysosomes and autophagosomes in the lutein cells compared to BCS3 cows. Cows with BCS2 or BCS1 had higher relative volume of lipid droplets, whilst the BCS4 or BCS5 cows had increased occurrence (relative volume) of vacuoles, lysosomes, peroxisomes and autolysosomes. These results show that luteolysis of cyclic CL of cows has different patterns in each BCS group; in the emaciated and fattened cows luteolysis starts earlier than in cows with average body condition. |
| first_indexed | 2024-12-20T23:11:39Z |
| format | Article |
| id | doaj.art-c8f35340a3f34501ba4ee8856cbae833 |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-12-20T23:11:39Z |
| publishDate | 2016-06-01 |
| publisher | Sciendo |
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| series | Acta Veterinaria |
| spelling | doaj.art-c8f35340a3f34501ba4ee8856cbae8332022-12-21T19:23:43ZengSciendoActa Veterinaria1820-74482016-06-0166224525610.1515/acve-2016-0021Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body ConditionPivko Juraj0Makarevich V. Alexander1Kubovičova Elena2Rafay Jan3Chrenek Peter4Research Institute for Animal Production Nitra, National Agricultural and Food Centre, Hlohovecka 2, 95141 Lužianky-near-Nitra, Slovak RepublicResearch Institute for Animal Production Nitra, National Agricultural and Food Centre, Hlohovecka 2, 95141 Lužianky-near-Nitra, Slovak RepublicResearch Institute for Animal Production Nitra, National Agricultural and Food Centre, Hlohovecka 2, 95141 Lužianky-near-Nitra, Slovak RepublicResearch Institute for Animal Production Nitra, National Agricultural and Food Centre, Hlohovecka 2, 95141 Lužianky-near-Nitra, Slovak RepublicResearch Institute for Animal Production Nitra, National Agricultural and Food Centre, Hlohovecka 2, 95141 Lužianky-near-Nitra, Slovak RepublicThe aim of the study was to define ultrastructural changes in the ovarian cyclic corpus luteum (CL) of dairy cows with different body condition score (BCS, a 5-point scale). The ovaries were recovered from slaughtered Holstein cows of BCS1 (emaciation; n= 4), BCS2 (tendency to emaciation; n=4), BCS3 (average body condition; n= 4), BCS4 (tendency to fattening; n=4) and BCS5 (fattening; n=4). Average ages of cows were: 6.2, 4.1, 5.7, 5.5 and 6.36 years for BCS1, BCS2, BCS3, BCS4 and BCS5, respectively. For electron microscopy, functioning CL at the mid-luteal phase were fixed in the aldehyde solution and post-fixed in 1% OsO4 solution. For lipid visualization the samples were soaked in 1% p-phenylenediamine solution in acetone. Durcupan ultrathin sections (90 nm) were contrasted with uranyl acetate and lead citrate and viewed under Jeol transmission electron microscope. Occurrence of cell organelles was determined by counting the relative volume of individual cellular components using the modified universal test network. Luteal regression of cyclic CL was characterized by atrophy of lutein cells, condensation of organelles and cytoplasmic inclusions, increase in relative volumes of vacuoles, lysosomes, peroxisomes, autophagosomes and lipids. There were significant differences (p<0.05) among BCS1, BCS2, BCS4 and BCS5 cows in the occurrence of lipids, vacuoles, lysosomes and autophagosomes in the lutein cells compared to BCS3 cows. Cows with BCS2 or BCS1 had higher relative volume of lipid droplets, whilst the BCS4 or BCS5 cows had increased occurrence (relative volume) of vacuoles, lysosomes, peroxisomes and autolysosomes. These results show that luteolysis of cyclic CL of cows has different patterns in each BCS group; in the emaciated and fattened cows luteolysis starts earlier than in cows with average body condition.https://doi.org/10.1515/acve-2016-0021body condition scorecattleemaciationfatteningluteal regression |
| spellingShingle | Pivko Juraj Makarevich V. Alexander Kubovičova Elena Rafay Jan Chrenek Peter Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition Acta Veterinaria body condition score cattle emaciation fattening luteal regression |
| title | Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition |
| title_full | Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition |
| title_fullStr | Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition |
| title_full_unstemmed | Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition |
| title_short | Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition |
| title_sort | ultrastructural changes in the cyclic corpus luteum of dairy cows with different body condition |
| topic | body condition score cattle emaciation fattening luteal regression |
| url | https://doi.org/10.1515/acve-2016-0021 |
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