Reduced DAXX Expression Is Associated with Reduced CD24 Expression in Colorectal Cancer
The presence of an activating mutation of the Wnt/β-catenin signaling pathway is found in ~90% of colorectal cancer (CRC) cases. Death domain-associated protein (DAXX), a nuclear protein, interacts with β-catenin in CRC cells. We investigated DAXX expression in 106 matched sample p...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/10/1242 |
| _version_ | 1797710528607420416 |
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| author | Ya-Chun Chen Tsung-Hsien Lee Shu-Ling Tzeng |
| author_facet | Ya-Chun Chen Tsung-Hsien Lee Shu-Ling Tzeng |
| author_sort | Ya-Chun Chen |
| collection | DOAJ |
| description | The presence of an activating mutation of the Wnt/β-catenin signaling pathway is found in ~90% of colorectal cancer (CRC) cases. Death domain-associated protein (DAXX), a nuclear protein, interacts with β-catenin in CRC cells. We investigated DAXX expression in 106 matched sample pairs of CRC and adjacent normal tissue by Western blotting. This study evaluated DAXX expression and its clinical implications in CRC. The results revealed that DAXX expression was significantly lower in the patients with the positive serum carcinoembryonic antigen (CEA) screening results compared to the patients with negative CEA screening levels (<i>p</i> < 0.001). It has been reported that CD24 is a Wnt target in CRC cells. Here, we further revealed that DAXX expression was significantly correlated with CD24 expression (rho = 0.360, <i>p</i> < 0.001) in 106 patients. Consistent with this, in the CEA-positive subgroup, of which the carcinomas expressed DAXX at low levels, they were significantly correlated with CD24 expression (rho = 0.461, <i>p</i> < 0.005). Therefore, reduced DAXX expression is associated with reduced CD24 expression in CRC. Notably, in the Hct116 cells, DAXX knockdown using short-hairpin RNA against DAXX (shDAXX) not only caused significant cell proliferation, but also promoted metastasis. The DAXX-knockdown cells also demonstrated significantly decreased CD24 expression, however the intracellular localization of CD24 did not change. Thus, DAXX might be considered as a potential regulator of CD24 or β-catenin expression, which might be correlated with proliferative and metastatic potential of CRC. |
| first_indexed | 2024-03-12T06:52:41Z |
| format | Article |
| id | doaj.art-c8f3a575fe12424bb84086bd230c8b61 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:52:41Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj.art-c8f3a575fe12424bb84086bd230c8b612023-09-03T00:11:17ZengMDPI AGCells2073-44092019-10-01810124210.3390/cells8101242cells8101242Reduced DAXX Expression Is Associated with Reduced CD24 Expression in Colorectal CancerYa-Chun Chen0Tsung-Hsien Lee1Shu-Ling Tzeng2Institute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanThe presence of an activating mutation of the Wnt/β-catenin signaling pathway is found in ~90% of colorectal cancer (CRC) cases. Death domain-associated protein (DAXX), a nuclear protein, interacts with β-catenin in CRC cells. We investigated DAXX expression in 106 matched sample pairs of CRC and adjacent normal tissue by Western blotting. This study evaluated DAXX expression and its clinical implications in CRC. The results revealed that DAXX expression was significantly lower in the patients with the positive serum carcinoembryonic antigen (CEA) screening results compared to the patients with negative CEA screening levels (<i>p</i> < 0.001). It has been reported that CD24 is a Wnt target in CRC cells. Here, we further revealed that DAXX expression was significantly correlated with CD24 expression (rho = 0.360, <i>p</i> < 0.001) in 106 patients. Consistent with this, in the CEA-positive subgroup, of which the carcinomas expressed DAXX at low levels, they were significantly correlated with CD24 expression (rho = 0.461, <i>p</i> < 0.005). Therefore, reduced DAXX expression is associated with reduced CD24 expression in CRC. Notably, in the Hct116 cells, DAXX knockdown using short-hairpin RNA against DAXX (shDAXX) not only caused significant cell proliferation, but also promoted metastasis. The DAXX-knockdown cells also demonstrated significantly decreased CD24 expression, however the intracellular localization of CD24 did not change. Thus, DAXX might be considered as a potential regulator of CD24 or β-catenin expression, which might be correlated with proliferative and metastatic potential of CRC.https://www.mdpi.com/2073-4409/8/10/1242colorectal cancerdaxxcd24carcinoembryonic antigenproliferationmetastasis |
| spellingShingle | Ya-Chun Chen Tsung-Hsien Lee Shu-Ling Tzeng Reduced DAXX Expression Is Associated with Reduced CD24 Expression in Colorectal Cancer Cells colorectal cancer daxx cd24 carcinoembryonic antigen proliferation metastasis |
| title | Reduced DAXX Expression Is Associated with Reduced CD24 Expression in Colorectal Cancer |
| title_full | Reduced DAXX Expression Is Associated with Reduced CD24 Expression in Colorectal Cancer |
| title_fullStr | Reduced DAXX Expression Is Associated with Reduced CD24 Expression in Colorectal Cancer |
| title_full_unstemmed | Reduced DAXX Expression Is Associated with Reduced CD24 Expression in Colorectal Cancer |
| title_short | Reduced DAXX Expression Is Associated with Reduced CD24 Expression in Colorectal Cancer |
| title_sort | reduced daxx expression is associated with reduced cd24 expression in colorectal cancer |
| topic | colorectal cancer daxx cd24 carcinoembryonic antigen proliferation metastasis |
| url | https://www.mdpi.com/2073-4409/8/10/1242 |
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