No Evidence for Binding Between Resistance Gene Product Cf-9 of Tomato and Avirulence Gene Product AVR9 of Cladosporium fulvum
The gene-for-gene model postulates that for every gene determining resistance in the host plant, there is a corresponding gene conditioning avirulence in the pathogen. On the basis of this relationship, products of resistance (R) genes and matching avirulence (Avr) genes are predicted to interact. H...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2001-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.7.867 |
| _version_ | 1818647113980968960 |
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| author | Rianne Luderer Susana Rivas Thorsten Nürnberger Benedetta Mattei Henno W. Van den Hooven Renier A. L. Van der Hoorn Tina Romeis Josa-M. Wehrfritz Beatrix Blume Dirk Nennstiel Douwe Zuidema Jacques Vervoort Giulia De Lorenzo Jonathan D. G. Jones Pierre J. G. M. De Wit Matthieu H. A. J. Joosten |
| author_facet | Rianne Luderer Susana Rivas Thorsten Nürnberger Benedetta Mattei Henno W. Van den Hooven Renier A. L. Van der Hoorn Tina Romeis Josa-M. Wehrfritz Beatrix Blume Dirk Nennstiel Douwe Zuidema Jacques Vervoort Giulia De Lorenzo Jonathan D. G. Jones Pierre J. G. M. De Wit Matthieu H. A. J. Joosten |
| author_sort | Rianne Luderer |
| collection | DOAJ |
| description | The gene-for-gene model postulates that for every gene determining resistance in the host plant, there is a corresponding gene conditioning avirulence in the pathogen. On the basis of this relationship, products of resistance (R) genes and matching avirulence (Avr) genes are predicted to interact. Here, we report on binding studies between the R gene product Cf-9 of tomato and the Avr gene product AVR9 of the pathogenic fungus Cladosporium fulvum. Because a high-affinity binding site (HABS) for AVR9 is present in tomato lines, with or without the Cf-9 resistance gene, as well as in other solanaceous plants, the Cf-9 protein was produced in COS and insect cells in order to perform binding studies in the absence of the HABS. Binding studies with radio-labeled AVR9 were performed with Cf-9-producing COS and insect cells and with membrane preparations of such cells. Furthermore, the Cf-9 gene was introduced in tobacco, which is known to be able to produce a functional Cf-9 protein. Binding of AVR9 to Cf-9 protein produced in tobacco was studied employing surface plasmon resonance and surface-enhanced laser desorption and ionization. Specific binding between Cf-9 and AVR9 was not detected with any of the procedures. The implications of this observation are discussed. |
| first_indexed | 2024-12-17T00:57:23Z |
| format | Article |
| id | doaj.art-c8f7b29e5df0491692d113e223f4daba |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:57:23Z |
| publishDate | 2001-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-c8f7b29e5df0491692d113e223f4daba2022-12-21T22:09:34ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062001-07-0114786787610.1094/MPMI.2001.14.7.867No Evidence for Binding Between Resistance Gene Product Cf-9 of Tomato and Avirulence Gene Product AVR9 of Cladosporium fulvumRianne LudererSusana RivasThorsten NürnbergerBenedetta MatteiHenno W. Van den HoovenRenier A. L. Van der HoornTina RomeisJosa-M. WehrfritzBeatrix BlumeDirk NennstielDouwe ZuidemaJacques VervoortGiulia De LorenzoJonathan D. G. JonesPierre J. G. M. De WitMatthieu H. A. J. JoostenThe gene-for-gene model postulates that for every gene determining resistance in the host plant, there is a corresponding gene conditioning avirulence in the pathogen. On the basis of this relationship, products of resistance (R) genes and matching avirulence (Avr) genes are predicted to interact. Here, we report on binding studies between the R gene product Cf-9 of tomato and the Avr gene product AVR9 of the pathogenic fungus Cladosporium fulvum. Because a high-affinity binding site (HABS) for AVR9 is present in tomato lines, with or without the Cf-9 resistance gene, as well as in other solanaceous plants, the Cf-9 protein was produced in COS and insect cells in order to perform binding studies in the absence of the HABS. Binding studies with radio-labeled AVR9 were performed with Cf-9-producing COS and insect cells and with membrane preparations of such cells. Furthermore, the Cf-9 gene was introduced in tobacco, which is known to be able to produce a functional Cf-9 protein. Binding of AVR9 to Cf-9 protein produced in tobacco was studied employing surface plasmon resonance and surface-enhanced laser desorption and ionization. Specific binding between Cf-9 and AVR9 was not detected with any of the procedures. The implications of this observation are discussed.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.7.867elicitorhypersensitive responseLRRreceptor |
| spellingShingle | Rianne Luderer Susana Rivas Thorsten Nürnberger Benedetta Mattei Henno W. Van den Hooven Renier A. L. Van der Hoorn Tina Romeis Josa-M. Wehrfritz Beatrix Blume Dirk Nennstiel Douwe Zuidema Jacques Vervoort Giulia De Lorenzo Jonathan D. G. Jones Pierre J. G. M. De Wit Matthieu H. A. J. Joosten No Evidence for Binding Between Resistance Gene Product Cf-9 of Tomato and Avirulence Gene Product AVR9 of Cladosporium fulvum Molecular Plant-Microbe Interactions elicitor hypersensitive response LRR receptor |
| title | No Evidence for Binding Between Resistance Gene Product Cf-9 of Tomato and Avirulence Gene Product AVR9 of Cladosporium fulvum |
| title_full | No Evidence for Binding Between Resistance Gene Product Cf-9 of Tomato and Avirulence Gene Product AVR9 of Cladosporium fulvum |
| title_fullStr | No Evidence for Binding Between Resistance Gene Product Cf-9 of Tomato and Avirulence Gene Product AVR9 of Cladosporium fulvum |
| title_full_unstemmed | No Evidence for Binding Between Resistance Gene Product Cf-9 of Tomato and Avirulence Gene Product AVR9 of Cladosporium fulvum |
| title_short | No Evidence for Binding Between Resistance Gene Product Cf-9 of Tomato and Avirulence Gene Product AVR9 of Cladosporium fulvum |
| title_sort | no evidence for binding between resistance gene product cf 9 of tomato and avirulence gene product avr9 of cladosporium fulvum |
| topic | elicitor hypersensitive response LRR receptor |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.7.867 |
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