Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus.
Misexpression of Polycomb repressive complex 1 (PRC1) components in human cells profoundly influences the onset of cellular senescence by modulating transcription of the INK4a tumor suppressor gene. Using tandem affinity purification, we find that CBX7 and CBX8, two Polycomb (Pc) homologs that repre...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-07-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2713427?pdf=render |
| _version_ | 1818108896467746816 |
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| author | Goedele N Maertens Selma El Messaoudi-Aubert Tomas Racek Julie K Stock James Nicholls Marc Rodriguez-Niedenführ Jesus Gil Gordon Peters |
| author_facet | Goedele N Maertens Selma El Messaoudi-Aubert Tomas Racek Julie K Stock James Nicholls Marc Rodriguez-Niedenführ Jesus Gil Gordon Peters |
| author_sort | Goedele N Maertens |
| collection | DOAJ |
| description | Misexpression of Polycomb repressive complex 1 (PRC1) components in human cells profoundly influences the onset of cellular senescence by modulating transcription of the INK4a tumor suppressor gene. Using tandem affinity purification, we find that CBX7 and CBX8, two Polycomb (Pc) homologs that repress INK4a, both participate in PRC1-like complexes with at least two Posterior sex combs (Psc) proteins, MEL18 and BMI1. Each complex contains a single representative of the Pc and Psc families. In primary human fibroblasts, CBX7, CBX8, MEL18 and BMI1 are present at the INK4a locus and shRNA-mediated knockdown of any one of these components results in de-repression of INK4a and proliferative arrest. Sequential chromatin immunoprecipitation (ChIP) reveals that CBX7 and CBX8 bind simultaneously to the same region of chromatin and knockdown of one of the Pc or Psc proteins results in release of the other, suggesting that the binding of PRC1 complexes is interdependent. Our findings provide the first evidence that a single gene can be regulated by several distinct PRC1 complexes and raise important questions about their configuration and relative functions. |
| first_indexed | 2024-12-11T02:22:38Z |
| format | Article |
| id | doaj.art-c8fee45a47a84dd9ac42b4409c63fa8a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T02:22:38Z |
| publishDate | 2009-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c8fee45a47a84dd9ac42b4409c63fa8a2022-12-22T01:24:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-07-0147e638010.1371/journal.pone.0006380Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus.Goedele N MaertensSelma El Messaoudi-AubertTomas RacekJulie K StockJames NichollsMarc Rodriguez-NiedenführJesus GilGordon PetersMisexpression of Polycomb repressive complex 1 (PRC1) components in human cells profoundly influences the onset of cellular senescence by modulating transcription of the INK4a tumor suppressor gene. Using tandem affinity purification, we find that CBX7 and CBX8, two Polycomb (Pc) homologs that repress INK4a, both participate in PRC1-like complexes with at least two Posterior sex combs (Psc) proteins, MEL18 and BMI1. Each complex contains a single representative of the Pc and Psc families. In primary human fibroblasts, CBX7, CBX8, MEL18 and BMI1 are present at the INK4a locus and shRNA-mediated knockdown of any one of these components results in de-repression of INK4a and proliferative arrest. Sequential chromatin immunoprecipitation (ChIP) reveals that CBX7 and CBX8 bind simultaneously to the same region of chromatin and knockdown of one of the Pc or Psc proteins results in release of the other, suggesting that the binding of PRC1 complexes is interdependent. Our findings provide the first evidence that a single gene can be regulated by several distinct PRC1 complexes and raise important questions about their configuration and relative functions.http://europepmc.org/articles/PMC2713427?pdf=render |
| spellingShingle | Goedele N Maertens Selma El Messaoudi-Aubert Tomas Racek Julie K Stock James Nicholls Marc Rodriguez-Niedenführ Jesus Gil Gordon Peters Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus. PLoS ONE |
| title | Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus. |
| title_full | Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus. |
| title_fullStr | Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus. |
| title_full_unstemmed | Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus. |
| title_short | Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus. |
| title_sort | several distinct polycomb complexes regulate and co localize on the ink4a tumor suppressor locus |
| url | http://europepmc.org/articles/PMC2713427?pdf=render |
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