Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers
Abstract Background Alterations of gut microbiota are associated with colorectal cancer (CRC) in different populations and several bacterial species were found to contribute to the tumorigenesis. The potential use of gut microbes as markers for early diagnosis has also been reported. However, cohort...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Microbiome |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40168-018-0451-2 |
| _version_ | 1818448669599334400 |
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| author | Zhenwei Dai Olabisi Oluwabukola Coker Geicho Nakatsu William K. K. Wu Liuyang Zhao Zigui Chen Francis K. L. Chan Karsten Kristiansen Joseph J. Y. Sung Sunny Hei Wong Jun Yu |
| author_facet | Zhenwei Dai Olabisi Oluwabukola Coker Geicho Nakatsu William K. K. Wu Liuyang Zhao Zigui Chen Francis K. L. Chan Karsten Kristiansen Joseph J. Y. Sung Sunny Hei Wong Jun Yu |
| author_sort | Zhenwei Dai |
| collection | DOAJ |
| description | Abstract Background Alterations of gut microbiota are associated with colorectal cancer (CRC) in different populations and several bacterial species were found to contribute to the tumorigenesis. The potential use of gut microbes as markers for early diagnosis has also been reported. However, cohort specific noises may distort the structure of microbial dysbiosis in CRC and lead to inconsistent results among studies. In this regard, our study targeted at exploring changes in gut microbiota that are universal across populations at species level. Results Based on the combined analysis of 526 metagenomic samples from Chinese, Austrian, American, and German and French cohorts, seven CRC-enriched bacteria (Bacteroides fragilis, Fusobacterium nucleatum, Porphyromonas asaccharolytica, Parvimonas micra, Prevotella intermedia, Alistipes finegoldii, and Thermanaerovibrio acidaminovorans) have been identified across populations. The seven enriched bacterial markers classified cases from controls with an area under the receiver-operating characteristics curve (AUC) of 0.80 across the different populations. Abundance correlation analysis demonstrated that CRC-enriched and CRC-depleted bacteria respectively formed their own mutualistic networks, in which the latter was disjointed in CRC. The CRC-enriched bacteria have been found to be correlated with lipopolysaccharide and energy biosynthetic pathways. Conclusions Our study identified potential diagnostic bacterial markers that are robust across populations, indicating their potential universal use for non-invasive CRC diagnosis. We also elucidated the ecological networks and functional capacities of CRC-associated microbiota. |
| first_indexed | 2024-12-14T20:23:11Z |
| format | Article |
| id | doaj.art-c900f2f00de04ded9e6840a936db069e |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-12-14T20:23:11Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-c900f2f00de04ded9e6840a936db069e2022-12-21T22:48:41ZengBMCMicrobiome2049-26182018-04-016111210.1186/s40168-018-0451-2Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markersZhenwei Dai0Olabisi Oluwabukola Coker1Geicho Nakatsu2William K. K. Wu3Liuyang Zhao4Zigui Chen5Francis K. L. Chan6Karsten Kristiansen7Joseph J. Y. Sung8Sunny Hei Wong9Jun Yu10Institute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong KongInstitute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong KongInstitute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong KongInstitute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong KongInstitute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong KongDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong KongInstitute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong KongLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of CopenhagenInstitute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong KongInstitute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong KongInstitute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong KongAbstract Background Alterations of gut microbiota are associated with colorectal cancer (CRC) in different populations and several bacterial species were found to contribute to the tumorigenesis. The potential use of gut microbes as markers for early diagnosis has also been reported. However, cohort specific noises may distort the structure of microbial dysbiosis in CRC and lead to inconsistent results among studies. In this regard, our study targeted at exploring changes in gut microbiota that are universal across populations at species level. Results Based on the combined analysis of 526 metagenomic samples from Chinese, Austrian, American, and German and French cohorts, seven CRC-enriched bacteria (Bacteroides fragilis, Fusobacterium nucleatum, Porphyromonas asaccharolytica, Parvimonas micra, Prevotella intermedia, Alistipes finegoldii, and Thermanaerovibrio acidaminovorans) have been identified across populations. The seven enriched bacterial markers classified cases from controls with an area under the receiver-operating characteristics curve (AUC) of 0.80 across the different populations. Abundance correlation analysis demonstrated that CRC-enriched and CRC-depleted bacteria respectively formed their own mutualistic networks, in which the latter was disjointed in CRC. The CRC-enriched bacteria have been found to be correlated with lipopolysaccharide and energy biosynthetic pathways. Conclusions Our study identified potential diagnostic bacterial markers that are robust across populations, indicating their potential universal use for non-invasive CRC diagnosis. We also elucidated the ecological networks and functional capacities of CRC-associated microbiota.http://link.springer.com/article/10.1186/s40168-018-0451-2Colorectal cancerMicrobiotaDiagnostic markerEcology |
| spellingShingle | Zhenwei Dai Olabisi Oluwabukola Coker Geicho Nakatsu William K. K. Wu Liuyang Zhao Zigui Chen Francis K. L. Chan Karsten Kristiansen Joseph J. Y. Sung Sunny Hei Wong Jun Yu Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers Microbiome Colorectal cancer Microbiota Diagnostic marker Ecology |
| title | Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers |
| title_full | Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers |
| title_fullStr | Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers |
| title_full_unstemmed | Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers |
| title_short | Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers |
| title_sort | multi cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers |
| topic | Colorectal cancer Microbiota Diagnostic marker Ecology |
| url | http://link.springer.com/article/10.1186/s40168-018-0451-2 |
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