Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection
Chlorophyll a/b-binding protein of light-harvesting complex II type 1 like (LHC II-1L) is an essential component of photosynthesis, which mainly maintains the stability of the electron transport chain. However, how the LHC II-1L protein of Fragaria vesca (FvLHC II-1L) affects viral infection remains...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.884044/full |
| _version_ | 1818534459142569984 |
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| author | Shiqiang Xu Xiangxiang Zhang Kai Xu Zhanqi Wang Xueping Zhou Lei Jiang Lei Jiang Lei Jiang Tong Jiang Tong Jiang Tong Jiang |
| author_facet | Shiqiang Xu Xiangxiang Zhang Kai Xu Zhanqi Wang Xueping Zhou Lei Jiang Lei Jiang Lei Jiang Tong Jiang Tong Jiang Tong Jiang |
| author_sort | Shiqiang Xu |
| collection | DOAJ |
| description | Chlorophyll a/b-binding protein of light-harvesting complex II type 1 like (LHC II-1L) is an essential component of photosynthesis, which mainly maintains the stability of the electron transport chain. However, how the LHC II-1L protein of Fragaria vesca (FvLHC II-1L) affects viral infection remains unclear. In this study, we demonstrated that the movement protein P1 of strawberry vein banding virus (SVBV P1) interacted with FvLHC II-1L in vivo and in vitro by bimolecular fluorescence complementation and pull-down assays. SVBV P1 was co-localized with FvLHC II-1L at the edge of epidermal cells of Nicotiana benthamiana leaves, and FvLHC II-1L protein expression was upregulated in SVBV-infected F. vesca. We also found that FvLHC II-1L effectively promoted SVBV P1 to compensate for the intercellular movement of movement-deficient potato virus X (PVXΔP25) and the systemic movement of movement-deficient cucumber mosaic virus (CMVΔMP). Transient overexpression of FvLHC II-1L and inoculation of an infectious clone of SVBV showed that the course of SVBV infection in F. vesca was accelerated. Collectively, the results showed that SVBV P1 protein can interact with FvLHC II-1L protein, which in turn promotes F. vesca infection by SVBV. |
| first_indexed | 2024-12-11T18:11:54Z |
| format | Article |
| id | doaj.art-c903a7615b4a4318b71f3583e0d1703f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T18:11:54Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c903a7615b4a4318b71f3583e0d1703f2022-12-22T00:55:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.884044884044Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral InfectionShiqiang Xu0Xiangxiang Zhang1Kai Xu2Zhanqi Wang3Xueping Zhou4Lei Jiang5Lei Jiang6Lei Jiang7Tong Jiang8Tong Jiang9Tong Jiang10Department of Plant Pathology, School of Plant Protection, Anhui Agricultural University, Hefei, ChinaDepartment of Plant Pathology, School of Plant Protection, Anhui Agricultural University, Hefei, ChinaDepartment of Plant Pathology, School of Plant Protection, Anhui Agricultural University, Hefei, ChinaKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, ChinaState Key Laboratory for Biology of Plant Disease and Insect Pest, Institute of Plant Protection, China Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Pathology, School of Plant Protection, Anhui Agricultural University, Hefei, ChinaAnhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei, ChinaKey Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei, ChinaDepartment of Plant Pathology, School of Plant Protection, Anhui Agricultural University, Hefei, ChinaAnhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei, ChinaKey Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei, ChinaChlorophyll a/b-binding protein of light-harvesting complex II type 1 like (LHC II-1L) is an essential component of photosynthesis, which mainly maintains the stability of the electron transport chain. However, how the LHC II-1L protein of Fragaria vesca (FvLHC II-1L) affects viral infection remains unclear. In this study, we demonstrated that the movement protein P1 of strawberry vein banding virus (SVBV P1) interacted with FvLHC II-1L in vivo and in vitro by bimolecular fluorescence complementation and pull-down assays. SVBV P1 was co-localized with FvLHC II-1L at the edge of epidermal cells of Nicotiana benthamiana leaves, and FvLHC II-1L protein expression was upregulated in SVBV-infected F. vesca. We also found that FvLHC II-1L effectively promoted SVBV P1 to compensate for the intercellular movement of movement-deficient potato virus X (PVXΔP25) and the systemic movement of movement-deficient cucumber mosaic virus (CMVΔMP). Transient overexpression of FvLHC II-1L and inoculation of an infectious clone of SVBV showed that the course of SVBV infection in F. vesca was accelerated. Collectively, the results showed that SVBV P1 protein can interact with FvLHC II-1L protein, which in turn promotes F. vesca infection by SVBV.https://www.frontiersin.org/articles/10.3389/fmicb.2022.884044/fullstrawberry vein banding virusintercellular movementFvLHC II-1L proteinP1 proteinviral infection |
| spellingShingle | Shiqiang Xu Xiangxiang Zhang Kai Xu Zhanqi Wang Xueping Zhou Lei Jiang Lei Jiang Lei Jiang Tong Jiang Tong Jiang Tong Jiang Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection Frontiers in Microbiology strawberry vein banding virus intercellular movement FvLHC II-1L protein P1 protein viral infection |
| title | Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection |
| title_full | Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection |
| title_fullStr | Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection |
| title_full_unstemmed | Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection |
| title_short | Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection |
| title_sort | strawberry vein banding virus movement protein p1 interacts with light harvesting complex ii type 1 like of fragaria vesca to promote viral infection |
| topic | strawberry vein banding virus intercellular movement FvLHC II-1L protein P1 protein viral infection |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.884044/full |
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