Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition
Abstract Background Osteoarthritis (OA) is a common degenerative joint disease characterized by persistent articular cartilage degeneration and synovitis. Oxymatrine (OMT) is a quinzolazine alkaloid extracted from the traditional Chinese medicine, matrine, and possesses anti-inflammatory properties...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-024-04667-2 |
| _version_ | 1827315870217535488 |
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| author | Jinying Lu Jiang Bian Yutong Wang Yan Zhao Xinmin Zhao Gao Wang Jing Yang |
| author_facet | Jinying Lu Jiang Bian Yutong Wang Yan Zhao Xinmin Zhao Gao Wang Jing Yang |
| author_sort | Jinying Lu |
| collection | DOAJ |
| description | Abstract Background Osteoarthritis (OA) is a common degenerative joint disease characterized by persistent articular cartilage degeneration and synovitis. Oxymatrine (OMT) is a quinzolazine alkaloid extracted from the traditional Chinese medicine, matrine, and possesses anti-inflammatory properties that may help regulate the pathogenesis of OA; however, its mechanism has not been elucidated. This study aimed to investigate the effects of OMT on interleukin-1β (IL-1β)-induced damage and the potential mechanisms of action. Methods Chondrocytes were isolated from Sprague-Dawley rats. Toluidine blue and Collagen II immunofluorescence staining were used to determine the purity of the chondrocytes. Thereafter, the chondrocytes were subjected to IL-1β stimulation, both in the presence and absence of OMT, or the autophagy inhibitor 3-methyladenine (3-MA). Cell viability was assessed using the MTT assay and SYTOX Green staining. Additionally, flow cytometry was used to determine cell apoptosis rate and reactive oxygen species (ROS) levels. The protein levels of AKT, mTOR, LC3, P62, matrix metalloproteinase-13, and collagen II were quantitatively analyzed using western blotting. Immunofluorescence was used to assess LC3 expression. Results OMT alleviated IL-1β-induced damage in chondrocytes, by increasing the survival rate, reducing the apoptosis rates of chondrocytes, and preventing the degradation of the cartilage matrix. In addition, OMT decreased the ROS levels and inhibited the AKT/mTOR signaling pathway while promoting autophagy in IL-1β treated chondrocytes. However, the effectiveness of OMT in improving chondrocyte viability under IL-1β treatment was limited when autophagy was inhibited by 3-MA. Conclusions OMT decreases oxidative stress and inhibits the AKT/mTOR signaling pathway to enhance autophagy, thus inhibiting IL-1β-induced damage. Therefore, OMT may be a novel and effective therapeutic agent for the clinical treatment of OA. |
| first_indexed | 2024-04-24T23:05:18Z |
| format | Article |
| id | doaj.art-c903d209e1d54083837fc9338f61544f |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-24T23:05:18Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-c903d209e1d54083837fc9338f61544f2024-03-17T12:32:53ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-03-0119111210.1186/s13018-024-04667-2Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibitionJinying Lu0Jiang Bian1Yutong Wang2Yan Zhao3Xinmin Zhao4Gao Wang5Jing Yang6Department of Biochemistry and Molecular Biology, Basic Medical College, Jinzhou Medical UniversityDepartment of Biochemistry and Molecular Biology, Basic Medical College, Jinzhou Medical UniversityDepartment of Biochemistry and Molecular Biology, Basic Medical College, Jinzhou Medical UniversityProvincial Key Laboratory of Cardiovascular and Cerebrovascular Drug Basic Research, Jinzhou Medical UniversityDepartment of Biochemistry and Molecular Biology, Basic Medical College, Jinzhou Medical UniversityDepartment of Biochemistry and Molecular Biology, Basic Medical College, Jinzhou Medical UniversityProvincial Key Laboratory of Cardiovascular and Cerebrovascular Drug Basic Research, Jinzhou Medical UniversityAbstract Background Osteoarthritis (OA) is a common degenerative joint disease characterized by persistent articular cartilage degeneration and synovitis. Oxymatrine (OMT) is a quinzolazine alkaloid extracted from the traditional Chinese medicine, matrine, and possesses anti-inflammatory properties that may help regulate the pathogenesis of OA; however, its mechanism has not been elucidated. This study aimed to investigate the effects of OMT on interleukin-1β (IL-1β)-induced damage and the potential mechanisms of action. Methods Chondrocytes were isolated from Sprague-Dawley rats. Toluidine blue and Collagen II immunofluorescence staining were used to determine the purity of the chondrocytes. Thereafter, the chondrocytes were subjected to IL-1β stimulation, both in the presence and absence of OMT, or the autophagy inhibitor 3-methyladenine (3-MA). Cell viability was assessed using the MTT assay and SYTOX Green staining. Additionally, flow cytometry was used to determine cell apoptosis rate and reactive oxygen species (ROS) levels. The protein levels of AKT, mTOR, LC3, P62, matrix metalloproteinase-13, and collagen II were quantitatively analyzed using western blotting. Immunofluorescence was used to assess LC3 expression. Results OMT alleviated IL-1β-induced damage in chondrocytes, by increasing the survival rate, reducing the apoptosis rates of chondrocytes, and preventing the degradation of the cartilage matrix. In addition, OMT decreased the ROS levels and inhibited the AKT/mTOR signaling pathway while promoting autophagy in IL-1β treated chondrocytes. However, the effectiveness of OMT in improving chondrocyte viability under IL-1β treatment was limited when autophagy was inhibited by 3-MA. Conclusions OMT decreases oxidative stress and inhibits the AKT/mTOR signaling pathway to enhance autophagy, thus inhibiting IL-1β-induced damage. Therefore, OMT may be a novel and effective therapeutic agent for the clinical treatment of OA.https://doi.org/10.1186/s13018-024-04667-2Oxymatrineinterleukin-1βChondrocytesAKT/mTOR pathwayAutophagy |
| spellingShingle | Jinying Lu Jiang Bian Yutong Wang Yan Zhao Xinmin Zhao Gao Wang Jing Yang Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition Journal of Orthopaedic Surgery and Research Oxymatrine interleukin-1β Chondrocytes AKT/mTOR pathway Autophagy |
| title | Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition |
| title_full | Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition |
| title_fullStr | Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition |
| title_full_unstemmed | Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition |
| title_short | Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition |
| title_sort | oxymatrine protects articular chondrocytes from il 1β induced damage through autophagy activation via akt mtor signaling pathway inhibition |
| topic | Oxymatrine interleukin-1β Chondrocytes AKT/mTOR pathway Autophagy |
| url | https://doi.org/10.1186/s13018-024-04667-2 |
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