Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation
Abstract Tailor made enzymatic preparation must be design to hydrolyze efficiently plant biomass, once that each plant biomass possesses a distinct cell wall composition. Most of actinomycetes used for plant cell wall degradation are focused on the cellulases and xylanases production. However, a wid...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100207&lng=en&tlng=en |
| _version_ | 1819207879863828480 |
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| author | Diogo Robl Carla Montanari Mergel Patricia dos Santos Costa José Geraldo da Cruz Pradella Gabriel Padilla |
| author_facet | Diogo Robl Carla Montanari Mergel Patricia dos Santos Costa José Geraldo da Cruz Pradella Gabriel Padilla |
| author_sort | Diogo Robl |
| collection | DOAJ |
| description | Abstract Tailor made enzymatic preparation must be design to hydrolyze efficiently plant biomass, once that each plant biomass possesses a distinct cell wall composition. Most of actinomycetes used for plant cell wall degradation are focused on the cellulases and xylanases production. However, a wide range of enzymes must be produced for an efficient degradation of lignocellulose materials. During the last decade several unusual environments were studied to obtain strains that produce glycohydrolases with innovator characteristics. In this context, the present work concerned the selection of endophytic actinomycetes as producers of hemicellulases and related enzymes with different enzymatic profiles, for use in the deconstruction of lignocellulosic biomass. A total of 45 Brazilian actinomycetes previously isolated from plants (endophytics) and soil were prospected for hemicellulases and β-glucosidase production. Four strains highlighted for hemicellulase production (DR61, DR63, DR69 and DR66) and were selected for cultivation under other inductors substrates (xylan and pectin). All strains belong to Streptomyces genera and have their extracts tested for degradation of several hemicellulolytic substrates. The strains presented different glicohydrolyse enzymes profiles mainly for xylans and glucans that can be used for specific formulations of enzymes applied on the biomass deconstruction, principally on sugar cane bagasse. |
| first_indexed | 2024-12-23T05:30:31Z |
| format | Article |
| id | doaj.art-c9040b3a2c244e04a53e24348bf4be1d |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-23T05:30:31Z |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-c9040b3a2c244e04a53e24348bf4be1d2022-12-21T17:58:29ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43246210.1590/1678-4324-2019180337S1516-89132019000100207Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass DegradationDiogo RoblCarla Montanari MergelPatricia dos Santos CostaJosé Geraldo da Cruz PradellaGabriel PadillaAbstract Tailor made enzymatic preparation must be design to hydrolyze efficiently plant biomass, once that each plant biomass possesses a distinct cell wall composition. Most of actinomycetes used for plant cell wall degradation are focused on the cellulases and xylanases production. However, a wide range of enzymes must be produced for an efficient degradation of lignocellulose materials. During the last decade several unusual environments were studied to obtain strains that produce glycohydrolases with innovator characteristics. In this context, the present work concerned the selection of endophytic actinomycetes as producers of hemicellulases and related enzymes with different enzymatic profiles, for use in the deconstruction of lignocellulosic biomass. A total of 45 Brazilian actinomycetes previously isolated from plants (endophytics) and soil were prospected for hemicellulases and β-glucosidase production. Four strains highlighted for hemicellulase production (DR61, DR63, DR69 and DR66) and were selected for cultivation under other inductors substrates (xylan and pectin). All strains belong to Streptomyces genera and have their extracts tested for degradation of several hemicellulolytic substrates. The strains presented different glicohydrolyse enzymes profiles mainly for xylans and glucans that can be used for specific formulations of enzymes applied on the biomass deconstruction, principally on sugar cane bagasse.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100207&lng=en&tlng=enEndophytic actinomyceteshemicellulasesaccessory enzymes |
| spellingShingle | Diogo Robl Carla Montanari Mergel Patricia dos Santos Costa José Geraldo da Cruz Pradella Gabriel Padilla Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation Brazilian Archives of Biology and Technology Endophytic actinomycetes hemicellulases accessory enzymes |
| title | Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation |
| title_full | Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation |
| title_fullStr | Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation |
| title_full_unstemmed | Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation |
| title_short | Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation |
| title_sort | endophytic actinomycetes as potential producers of hemicellulases and related enzymes for plant biomass degradation |
| topic | Endophytic actinomycetes hemicellulases accessory enzymes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100207&lng=en&tlng=en |
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