Effect of Neuropeptide S Administration on Ultrasonic Vocalizations and Behaviour in Rats with Low vs. High Exploratory Activity
Neuropeptide S (NPS) is a peptide neurotransmitter that in animal studies promotes wakefulness and arousal with simultaneous anxiety reduction, in some inconsistency with results in humans. We examined the effect of NPS on rat ultrasonic vocalizations (USV) as an index of affective state and on beha...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/14/6/524 |
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| author | Kadri Kõiv Denis Matrov Trine Uusen Jaanus Harro |
| author_facet | Kadri Kõiv Denis Matrov Trine Uusen Jaanus Harro |
| author_sort | Kadri Kõiv |
| collection | DOAJ |
| description | Neuropeptide S (NPS) is a peptide neurotransmitter that in animal studies promotes wakefulness and arousal with simultaneous anxiety reduction, in some inconsistency with results in humans. We examined the effect of NPS on rat ultrasonic vocalizations (USV) as an index of affective state and on behaviour in novel environments in rats with persistent inter-individual differences in exploratory activity. Adult male Wistar rats were categorised as of high (HE) or low (LE) exploratory activity and NPS was administered intracerebroventricularly (i.c.v.) at a dose of 1.0 nmol/5 µL, after which USVs were recorded in the home-cage and a novel standard housing cage, and behaviour evaluated in exploration/anxiety tests. NPS induced a massive production of long and short 22 kHz USVs in the home cage that continued later in the novel environment; no effect on 50 kHz USVs were found. In LE-rats, the long 22 kHz calls were emitted at lower frequencies and were louder. The effects of NPS on behaviour appeared novelty- and test-dependent. NPS had an anxiolytic-like effect in LE-rats only in the elevated zero-maze, whereas in HE-rats, locomotor activity in the zero-maze and in a novel standard cage was increased. Thus NPS appears as a psychostimulant peptide but with a complex effect on dimensions of affect. |
| first_indexed | 2024-03-10T10:54:03Z |
| format | Article |
| id | doaj.art-c905f2eb15634cab958a7a4b567d4e8e |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T10:54:03Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-c905f2eb15634cab958a7a4b567d4e8e2023-11-21T22:04:08ZengMDPI AGPharmaceuticals1424-82472021-05-0114652410.3390/ph14060524Effect of Neuropeptide S Administration on Ultrasonic Vocalizations and Behaviour in Rats with Low vs. High Exploratory ActivityKadri Kõiv0Denis Matrov1Trine Uusen2Jaanus Harro3Institute of Psychology, University of Tartu, Ravila 14A, 50411 Tartu, EstoniaThe Centre of Excellence in Neural and Behavioural Sciences, School of Natural Sciences and Health, Tallinn University, Narva Rd. 29, 10120 Tallinn, EstoniaInstitute of Psychology, University of Tartu, Ravila 14A, 50411 Tartu, EstoniaChair of Neuropsychopharmacology, Institute of Chemistry, University of Tartu, Ravila 14A, 50411 Tartu, EstoniaNeuropeptide S (NPS) is a peptide neurotransmitter that in animal studies promotes wakefulness and arousal with simultaneous anxiety reduction, in some inconsistency with results in humans. We examined the effect of NPS on rat ultrasonic vocalizations (USV) as an index of affective state and on behaviour in novel environments in rats with persistent inter-individual differences in exploratory activity. Adult male Wistar rats were categorised as of high (HE) or low (LE) exploratory activity and NPS was administered intracerebroventricularly (i.c.v.) at a dose of 1.0 nmol/5 µL, after which USVs were recorded in the home-cage and a novel standard housing cage, and behaviour evaluated in exploration/anxiety tests. NPS induced a massive production of long and short 22 kHz USVs in the home cage that continued later in the novel environment; no effect on 50 kHz USVs were found. In LE-rats, the long 22 kHz calls were emitted at lower frequencies and were louder. The effects of NPS on behaviour appeared novelty- and test-dependent. NPS had an anxiolytic-like effect in LE-rats only in the elevated zero-maze, whereas in HE-rats, locomotor activity in the zero-maze and in a novel standard cage was increased. Thus NPS appears as a psychostimulant peptide but with a complex effect on dimensions of affect.https://www.mdpi.com/1424-8247/14/6/524neuropeptide S (NPS)exploratory activityultrasonic vocalizations (USVs)50 kHz USVslong and short 22 kHz USVsindividual differences |
| spellingShingle | Kadri Kõiv Denis Matrov Trine Uusen Jaanus Harro Effect of Neuropeptide S Administration on Ultrasonic Vocalizations and Behaviour in Rats with Low vs. High Exploratory Activity Pharmaceuticals neuropeptide S (NPS) exploratory activity ultrasonic vocalizations (USVs) 50 kHz USVs long and short 22 kHz USVs individual differences |
| title | Effect of Neuropeptide S Administration on Ultrasonic Vocalizations and Behaviour in Rats with Low vs. High Exploratory Activity |
| title_full | Effect of Neuropeptide S Administration on Ultrasonic Vocalizations and Behaviour in Rats with Low vs. High Exploratory Activity |
| title_fullStr | Effect of Neuropeptide S Administration on Ultrasonic Vocalizations and Behaviour in Rats with Low vs. High Exploratory Activity |
| title_full_unstemmed | Effect of Neuropeptide S Administration on Ultrasonic Vocalizations and Behaviour in Rats with Low vs. High Exploratory Activity |
| title_short | Effect of Neuropeptide S Administration on Ultrasonic Vocalizations and Behaviour in Rats with Low vs. High Exploratory Activity |
| title_sort | effect of neuropeptide s administration on ultrasonic vocalizations and behaviour in rats with low vs high exploratory activity |
| topic | neuropeptide S (NPS) exploratory activity ultrasonic vocalizations (USVs) 50 kHz USVs long and short 22 kHz USVs individual differences |
| url | https://www.mdpi.com/1424-8247/14/6/524 |
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