Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology
Circular RNAs (circRNAs) are a recently characterized family of gene transcripts forming a covalently closed loop of single-stranded RNA. The extent of their potential for fine-tuning gene expression is still being discovered. Several studies have implicated certain circular RNAs in pathophysiologic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/680 |
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| author | Leïla Halidou Diallo Jérôme Mariette Nathalie Laugero Christian Touriol Florent Morfoisse Anne-Catherine Prats Barbara Garmy-Susini Eric Lacazette |
| author_facet | Leïla Halidou Diallo Jérôme Mariette Nathalie Laugero Christian Touriol Florent Morfoisse Anne-Catherine Prats Barbara Garmy-Susini Eric Lacazette |
| author_sort | Leïla Halidou Diallo |
| collection | DOAJ |
| description | Circular RNAs (circRNAs) are a recently characterized family of gene transcripts forming a covalently closed loop of single-stranded RNA. The extent of their potential for fine-tuning gene expression is still being discovered. Several studies have implicated certain circular RNAs in pathophysiological processes within vascular endothelial cells and cancer cells independently. However, to date, no comparative study of circular RNA expression in different types of endothelial cells has been performed and analysed through the lens of their central role in vascular physiology and pathology. In this work, we analysed publicly available and original RNA sequencing datasets from arterial, veinous, and lymphatic endothelial cells to identify common and distinct circRNA expression profiles. We identified 4713 distinct circRNAs in the compared endothelial cell types, 95% of which originated from exons. Interestingly, the results show that the expression profile of circular RNAs is much more specific to each cell type than linear RNAs, and therefore appears to be more suitable for distinguishing between them. As a result, we have discovered a specific circRNA signature for each given endothelial cell type. Furthermore, we identified a specific endothelial cell circRNA signature that is composed four circRNAs: circCARD6, circPLXNA2, circCASC15 and circEPHB4. These circular RNAs are produced by genes that are related to endothelial cell migration pathways and cancer progression. More detailed studies of their functions could lead to a better understanding of the mechanisms involved in physiological and pathological (lymph)angiogenesis and might open new ways to tackle tumour spread through the vascular system. |
| first_indexed | 2024-03-08T15:04:22Z |
| format | Article |
| id | doaj.art-c907e56eb99b4a6fa870f70b62e7d0d9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:04:22Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c907e56eb99b4a6fa870f70b62e7d0d92024-01-10T15:00:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-0125168010.3390/ijms25010680Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular PathophysiologyLeïla Halidou Diallo0Jérôme Mariette1Nathalie Laugero2Christian Touriol3Florent Morfoisse4Anne-Catherine Prats5Barbara Garmy-Susini6Eric Lacazette7U1297-I2MC, INSERM, University of Toulouse, 1 Avenue Jean Poulhes, BP 84225, 31432 Toulouse, FranceMIAT, University of Toulouse, INRAE, 31326 Castanet-Tolosan, FranceU1297-I2MC, INSERM, University of Toulouse, 1 Avenue Jean Poulhes, BP 84225, 31432 Toulouse, FranceUMR1037 INSERM, University of Toulouse, 2 Avenue Hubert Curien, 31100 Toulouse, FranceU1297-I2MC, INSERM, University of Toulouse, 1 Avenue Jean Poulhes, BP 84225, 31432 Toulouse, FranceU1297-I2MC, INSERM, University of Toulouse, 1 Avenue Jean Poulhes, BP 84225, 31432 Toulouse, FranceU1297-I2MC, INSERM, University of Toulouse, 1 Avenue Jean Poulhes, BP 84225, 31432 Toulouse, FranceU1297-I2MC, INSERM, University of Toulouse, 1 Avenue Jean Poulhes, BP 84225, 31432 Toulouse, FranceCircular RNAs (circRNAs) are a recently characterized family of gene transcripts forming a covalently closed loop of single-stranded RNA. The extent of their potential for fine-tuning gene expression is still being discovered. Several studies have implicated certain circular RNAs in pathophysiological processes within vascular endothelial cells and cancer cells independently. However, to date, no comparative study of circular RNA expression in different types of endothelial cells has been performed and analysed through the lens of their central role in vascular physiology and pathology. In this work, we analysed publicly available and original RNA sequencing datasets from arterial, veinous, and lymphatic endothelial cells to identify common and distinct circRNA expression profiles. We identified 4713 distinct circRNAs in the compared endothelial cell types, 95% of which originated from exons. Interestingly, the results show that the expression profile of circular RNAs is much more specific to each cell type than linear RNAs, and therefore appears to be more suitable for distinguishing between them. As a result, we have discovered a specific circRNA signature for each given endothelial cell type. Furthermore, we identified a specific endothelial cell circRNA signature that is composed four circRNAs: circCARD6, circPLXNA2, circCASC15 and circEPHB4. These circular RNAs are produced by genes that are related to endothelial cell migration pathways and cancer progression. More detailed studies of their functions could lead to a better understanding of the mechanisms involved in physiological and pathological (lymph)angiogenesis and might open new ways to tackle tumour spread through the vascular system.https://www.mdpi.com/1422-0067/25/1/680circRNAendothelial cell(lymph)angiogenesisgene signature |
| spellingShingle | Leïla Halidou Diallo Jérôme Mariette Nathalie Laugero Christian Touriol Florent Morfoisse Anne-Catherine Prats Barbara Garmy-Susini Eric Lacazette Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology International Journal of Molecular Sciences circRNA endothelial cell (lymph)angiogenesis gene signature |
| title | Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology |
| title_full | Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology |
| title_fullStr | Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology |
| title_full_unstemmed | Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology |
| title_short | Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology |
| title_sort | specific circular rna signature of endothelial cells potential implications in vascular pathophysiology |
| topic | circRNA endothelial cell (lymph)angiogenesis gene signature |
| url | https://www.mdpi.com/1422-0067/25/1/680 |
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