Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt Disease
Microbial volatiles benefit the agricultural ecological system by promoting plant growth and systemic resistance against diseases without harming the environment. To explore the plant growth-promoting efficiency of VOCs produced by <i>Pseudomonas fluorescens</i> PDS1 and <i>Bacillu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/2/404 |
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| author | Abhijeet Shankar Kashyap Nazia Manzar Suresh M. Nebapure Mahendra Vikram Singh Rajawat Man Mohan Deo Jyoti Prakash Singh Amit Kumar Kesharwani Ravinder Pal Singh S. C. Dubey Dinesh Singh |
| author_facet | Abhijeet Shankar Kashyap Nazia Manzar Suresh M. Nebapure Mahendra Vikram Singh Rajawat Man Mohan Deo Jyoti Prakash Singh Amit Kumar Kesharwani Ravinder Pal Singh S. C. Dubey Dinesh Singh |
| author_sort | Abhijeet Shankar Kashyap |
| collection | DOAJ |
| description | Microbial volatiles benefit the agricultural ecological system by promoting plant growth and systemic resistance against diseases without harming the environment. To explore the plant growth-promoting efficiency of VOCs produced by <i>Pseudomonas fluorescens</i> PDS1 and <i>Bacillus subtilis</i> KA9 in terms of chili plant growth and its biocontrol efficiency against <i>Ralstonia solanacearum</i>, experiments were conducted both in vitro and in vivo. A closure assembly was designed using a half-inverted plastic bottle to demonstrate plant–microbial interactions via volatile compounds. The most common volatile organic compounds were identified and reported; they promoted plant development and induced systemic resistance (ISR) against wilt pathogen <i>R. solanacearum</i>. The PDS1 and KA9 VOCs significantly increased defensive enzyme activity and overexpressed the antioxidant genes PAL, POD, SOD, WRKYa, PAL1, DEF-1, CAT-2, WRKY40, HSFC1, LOX2, and NPR1 related to plant defense. The overall gene expression was greater in root tissue as compared to leaf tissue in chili plant. Our findings shed light on the relationship among rhizobacteria, pathogen, and host plants, resulting in plant growth promotion, disease suppression, systemic resistance-inducing potential, and antioxidant response with related gene expression in the leaf and root tissue of chili. |
| first_indexed | 2024-03-09T22:43:52Z |
| format | Article |
| id | doaj.art-c90bb3c2494d40049cd66a262481d0d6 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T22:43:52Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-c90bb3c2494d40049cd66a262481d0d62023-11-23T18:33:18ZengMDPI AGAntioxidants2076-39212022-02-0111240410.3390/antiox11020404Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt DiseaseAbhijeet Shankar Kashyap0Nazia Manzar1Suresh M. Nebapure2Mahendra Vikram Singh Rajawat3Man Mohan Deo4Jyoti Prakash Singh5Amit Kumar Kesharwani6Ravinder Pal Singh7S. C. Dubey8Dinesh Singh9Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaPlant Pathology Laboratory, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan 275103, IndiaDivision of Entomology, ICAR-IARI, New Delhi 110012, IndiaPlant Pathology Laboratory, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan 275103, IndiaFarm Machinery and Power, ICAR-Indian Institute of Pulses Research, Kanpur 208024, IndiaPlant Pathology Laboratory, ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan 275103, IndiaDivision of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Plant Quarantine, ICAR-NBPGR, New Delhi 110012, IndiaDivision of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaMicrobial volatiles benefit the agricultural ecological system by promoting plant growth and systemic resistance against diseases without harming the environment. To explore the plant growth-promoting efficiency of VOCs produced by <i>Pseudomonas fluorescens</i> PDS1 and <i>Bacillus subtilis</i> KA9 in terms of chili plant growth and its biocontrol efficiency against <i>Ralstonia solanacearum</i>, experiments were conducted both in vitro and in vivo. A closure assembly was designed using a half-inverted plastic bottle to demonstrate plant–microbial interactions via volatile compounds. The most common volatile organic compounds were identified and reported; they promoted plant development and induced systemic resistance (ISR) against wilt pathogen <i>R. solanacearum</i>. The PDS1 and KA9 VOCs significantly increased defensive enzyme activity and overexpressed the antioxidant genes PAL, POD, SOD, WRKYa, PAL1, DEF-1, CAT-2, WRKY40, HSFC1, LOX2, and NPR1 related to plant defense. The overall gene expression was greater in root tissue as compared to leaf tissue in chili plant. Our findings shed light on the relationship among rhizobacteria, pathogen, and host plants, resulting in plant growth promotion, disease suppression, systemic resistance-inducing potential, and antioxidant response with related gene expression in the leaf and root tissue of chili.https://www.mdpi.com/2076-3921/11/2/404<i>B. subtilis</i> KA9volatile compoundsGC–MS spectroscopysystemic resistance<i>P. fluorescens</i> PDS1real-time PCR |
| spellingShingle | Abhijeet Shankar Kashyap Nazia Manzar Suresh M. Nebapure Mahendra Vikram Singh Rajawat Man Mohan Deo Jyoti Prakash Singh Amit Kumar Kesharwani Ravinder Pal Singh S. C. Dubey Dinesh Singh Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt Disease Antioxidants <i>B. subtilis</i> KA9 volatile compounds GC–MS spectroscopy systemic resistance <i>P. fluorescens</i> PDS1 real-time PCR |
| title | Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt Disease |
| title_full | Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt Disease |
| title_fullStr | Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt Disease |
| title_full_unstemmed | Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt Disease |
| title_short | Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt Disease |
| title_sort | unraveling microbial volatile elicitors using a transparent methodology for induction of systemic resistance and regulation of antioxidant genes at expression levels in chili against bacterial wilt disease |
| topic | <i>B. subtilis</i> KA9 volatile compounds GC–MS spectroscopy systemic resistance <i>P. fluorescens</i> PDS1 real-time PCR |
| url | https://www.mdpi.com/2076-3921/11/2/404 |
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