Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking
In this research, green synthesized magnesium oxide nanoparticles (MgO NPs) from lemon fruit extracts and their fungicidal potential was evaluated against Alternaria dauci infection on carrot (Daucus carota L.) under greenhouse conditions. The scanning and transmission electron microscopy (SEM and T...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923000461 |
| _version_ | 1797660333228163072 |
|---|---|
| author | Lukman AHAMAD Azmat ALI KHAN Masudulla KHAN Orudzhev FARID Mahboob ALAM |
| author_facet | Lukman AHAMAD Azmat ALI KHAN Masudulla KHAN Orudzhev FARID Mahboob ALAM |
| author_sort | Lukman AHAMAD |
| collection | DOAJ |
| description | In this research, green synthesized magnesium oxide nanoparticles (MgO NPs) from lemon fruit extracts and their fungicidal potential was evaluated against Alternaria dauci infection on carrot (Daucus carota L.) under greenhouse conditions. The scanning and transmission electron microscopy (SEM and TEM) and ultra-violet (UV) visible spectroscopy were used to validate and characterize MgO NPs. The crystalline nature of MgONPs was determined using selected area electron diffraction (SAED). MgO NPs triggered substantial antifungal activity against A. dauci when exposed to 50 and 100 mg L−1 concentrations but the higher antifungal potential was noticed in 100 mg L−1 under in-vitro conditions. In fungal inoculated plants, a marked decrease in growth, photosynthetic pigments, and an increase in phenol, proline contents, and defense-related enzymes of carrot were seen over control (distilled water). However, foliar application of MgO NPs at 50 and 100 mg L−1 resulted in significant improvement of plant growth, photosynthetic pigments, phenol and proline contents, and defense enzymes activity of carrots with and without A. dauci infection. Spraying of MgO NPs at 100 mg L−1 had more plant length (17.11%), shoot dry weight (34.38%), plant fresh weight (20.46%), and root dry weight (49.09%) in carrots when challenged with A. dauci over inoculated control. The leaf blight indices and percent disease severity were also reduced in A. dauci inoculated plants when sprayed with MgO NPs. The non-bonding interactions of Alternaria genus protein with nanoparticles were studied using molecular docking. |
| first_indexed | 2024-03-11T18:29:17Z |
| format | Article |
| id | doaj.art-c90e128698b94942b6397cf870afbe04 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-11T18:29:17Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-c90e128698b94942b6397cf870afbe042023-10-13T13:53:12ZengElsevierJournal of Integrative Agriculture2095-31192023-10-01221030693080Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular dockingLukman AHAMAD0Azmat ALI KHAN1Masudulla KHAN2Orudzhev FARID3Mahboob ALAM4Plant Pathology/Nematology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202002, India; Regional Plant Quarantine Station, Salt Lake, Kolkata 700097, IndiaPharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaPlant Pathology/Nematology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202002, India; Botany Section, Women's College, Aligarh Muslim University, Aligarh 202002, India; Correspondence Masudulla KHANAmirkhanov Institute of Physics, Dagestan Federal Scientific Center, Russian Academy of Sciences, Makhachkala 367003, RussiaDepartment of Safety Engineering, Dongguk University, Gyeongju-si 780714, Republic of Korea; Correspondence Mahboob ALAMIn this research, green synthesized magnesium oxide nanoparticles (MgO NPs) from lemon fruit extracts and their fungicidal potential was evaluated against Alternaria dauci infection on carrot (Daucus carota L.) under greenhouse conditions. The scanning and transmission electron microscopy (SEM and TEM) and ultra-violet (UV) visible spectroscopy were used to validate and characterize MgO NPs. The crystalline nature of MgONPs was determined using selected area electron diffraction (SAED). MgO NPs triggered substantial antifungal activity against A. dauci when exposed to 50 and 100 mg L−1 concentrations but the higher antifungal potential was noticed in 100 mg L−1 under in-vitro conditions. In fungal inoculated plants, a marked decrease in growth, photosynthetic pigments, and an increase in phenol, proline contents, and defense-related enzymes of carrot were seen over control (distilled water). However, foliar application of MgO NPs at 50 and 100 mg L−1 resulted in significant improvement of plant growth, photosynthetic pigments, phenol and proline contents, and defense enzymes activity of carrots with and without A. dauci infection. Spraying of MgO NPs at 100 mg L−1 had more plant length (17.11%), shoot dry weight (34.38%), plant fresh weight (20.46%), and root dry weight (49.09%) in carrots when challenged with A. dauci over inoculated control. The leaf blight indices and percent disease severity were also reduced in A. dauci inoculated plants when sprayed with MgO NPs. The non-bonding interactions of Alternaria genus protein with nanoparticles were studied using molecular docking.http://www.sciencedirect.com/science/article/pii/S2095311923000461green synthesisMgO nanoparticlesalternaria leaf blightphotosynthetic pigmentsdefense enzymescarrot |
| spellingShingle | Lukman AHAMAD Azmat ALI KHAN Masudulla KHAN Orudzhev FARID Mahboob ALAM Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking Journal of Integrative Agriculture green synthesis MgO nanoparticles alternaria leaf blight photosynthetic pigments defense enzymes carrot |
| title | Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking |
| title_full | Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking |
| title_fullStr | Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking |
| title_full_unstemmed | Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking |
| title_short | Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking |
| title_sort | exploring the nano fungicidal efficacy of green synthesized magnesium oxide nanoparticles mgo nps on the development physiology and infection of carrot daucus carota l with alternaria leaf blight alb molecular docking |
| topic | green synthesis MgO nanoparticles alternaria leaf blight photosynthetic pigments defense enzymes carrot |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923000461 |
| work_keys_str_mv | AT lukmanahamad exploringthenanofungicidalefficacyofgreensynthesizedmagnesiumoxidenanoparticlesmgonpsonthedevelopmentphysiologyandinfectionofcarrotdaucuscarotalwithalternarialeafblightalbmoleculardocking AT azmatalikhan exploringthenanofungicidalefficacyofgreensynthesizedmagnesiumoxidenanoparticlesmgonpsonthedevelopmentphysiologyandinfectionofcarrotdaucuscarotalwithalternarialeafblightalbmoleculardocking AT masudullakhan exploringthenanofungicidalefficacyofgreensynthesizedmagnesiumoxidenanoparticlesmgonpsonthedevelopmentphysiologyandinfectionofcarrotdaucuscarotalwithalternarialeafblightalbmoleculardocking AT orudzhevfarid exploringthenanofungicidalefficacyofgreensynthesizedmagnesiumoxidenanoparticlesmgonpsonthedevelopmentphysiologyandinfectionofcarrotdaucuscarotalwithalternarialeafblightalbmoleculardocking AT mahboobalam exploringthenanofungicidalefficacyofgreensynthesizedmagnesiumoxidenanoparticlesmgonpsonthedevelopmentphysiologyandinfectionofcarrotdaucuscarotalwithalternarialeafblightalbmoleculardocking |