Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)
Microbial diversity within an aquatic community can be used to increase the growth and development of organisms. In this study, Zebrafish larvae were reared in three treatments 1) a probiotic containing 17 strains of Lactobacillus spp., 2) an amoxicillin solution, 3) water from the broodstock cultur...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2021-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/98/e3sconf_isffs2021_02016.pdf |
| _version_ | 1818398326765125632 |
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| author | Padeniya Uthpala Septriani Shafira Pataueg Arjay Brown Christopher L. |
| author_facet | Padeniya Uthpala Septriani Shafira Pataueg Arjay Brown Christopher L. |
| author_sort | Padeniya Uthpala |
| collection | DOAJ |
| description | Microbial diversity within an aquatic community can be used to increase the growth and development of organisms. In this study, Zebrafish larvae were reared in three treatments 1) a probiotic containing 17 strains of Lactobacillus spp., 2) an amoxicillin solution, 3) water from the broodstock culture tank as the control. Survival of the larvae throughout 10 dpf was recorded. To determine the development of the gastrointestinal tract DASPEI stain was used for larvae at the age of 3 dpf to 6dpf. The intensity of the fluorescence in each larva was observed through the automated digital microscope. According to the experiment results, a significant difference (P<0.0001) in the survival rates among all treatments was recorded. The probiotic-treated larvae (PTL) had a higher survival rate. This could be due to the presence of lactic acid bacteria in the probiotic treatment, which helps in enhancing immunity. In DASPEI staining, also PTL exhibits more fluorescence in the GI tract at 24 hours to 48 hours post-hatch than the other two treatments. The main possible reason behind this could also be the presence of Lactobacillus spp. Which directly influences the higher activity of the digestive system. |
| first_indexed | 2024-12-14T07:03:01Z |
| format | Article |
| id | doaj.art-c91232ee22d14a298885a106509bcb94 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-14T07:03:01Z |
| publishDate | 2021-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-c91232ee22d14a298885a106509bcb942022-12-21T23:12:22ZengEDP SciencesE3S Web of Conferences2267-12422021-01-013220201610.1051/e3sconf/202132202016e3sconf_isffs2021_02016Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)Padeniya Uthpala0Septriani Shafira1Pataueg Arjay2Brown Christopher L.3World Fisheries University Pilot Programme, Pukyong National University (PKNU)World Fisheries University Pilot Programme, Pukyong National University (PKNU)World Fisheries University Pilot Programme, Pukyong National University (PKNU)World Fisheries University Pilot Programme, Pukyong National University (PKNU)Microbial diversity within an aquatic community can be used to increase the growth and development of organisms. In this study, Zebrafish larvae were reared in three treatments 1) a probiotic containing 17 strains of Lactobacillus spp., 2) an amoxicillin solution, 3) water from the broodstock culture tank as the control. Survival of the larvae throughout 10 dpf was recorded. To determine the development of the gastrointestinal tract DASPEI stain was used for larvae at the age of 3 dpf to 6dpf. The intensity of the fluorescence in each larva was observed through the automated digital microscope. According to the experiment results, a significant difference (P<0.0001) in the survival rates among all treatments was recorded. The probiotic-treated larvae (PTL) had a higher survival rate. This could be due to the presence of lactic acid bacteria in the probiotic treatment, which helps in enhancing immunity. In DASPEI staining, also PTL exhibits more fluorescence in the GI tract at 24 hours to 48 hours post-hatch than the other two treatments. The main possible reason behind this could also be the presence of Lactobacillus spp. Which directly influences the higher activity of the digestive system.https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/98/e3sconf_isffs2021_02016.pdf |
| spellingShingle | Padeniya Uthpala Septriani Shafira Pataueg Arjay Brown Christopher L. Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio) E3S Web of Conferences |
| title | Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio) |
| title_full | Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio) |
| title_fullStr | Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio) |
| title_full_unstemmed | Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio) |
| title_short | Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio) |
| title_sort | effects of the microbiome manipulation on survival and gi tract development of larval zebrafish danio rerio |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/98/e3sconf_isffs2021_02016.pdf |
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