USE OF NATIVE CELLULOLYTIC ENZYMES FROM ECUADOR, IN THE ENZYMATIC HYDROLYSIS FOR THE ETHANOLPRODUCTION
This study shows the use of cellulolytic enzymes Bacillus sp.in enzymatic hydrolysis process and their use in the production of second-generation ethanol. For doing this, The Plackett-Burman method was used to discard variables that do not influence the enzymatic hydrolysis process, and subsequently...
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| Format: | Article |
| Language: | English |
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Universidad Central "Marta Abreu" de Las Villas
2018-09-01
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| Series: | Revista Centro Azúcar |
| Subjects: | |
| Online Access: | http://centroazucar.uclv.edu.cu/index.php/centro_azucar/article/view/75 |
| _version_ | 1811227939422863360 |
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| author | Jonathan Villavicencio Montoya Carmen Amelia Salvador Pinos Fernando Batallas Merino Amaury Pérez Martínez Layanis Mesa Garriga Erenio González Suárez |
| author_facet | Jonathan Villavicencio Montoya Carmen Amelia Salvador Pinos Fernando Batallas Merino Amaury Pérez Martínez Layanis Mesa Garriga Erenio González Suárez |
| author_sort | Jonathan Villavicencio Montoya |
| collection | DOAJ |
| description | This study shows the use of cellulolytic enzymes Bacillus sp.in enzymatic hydrolysis process and their use in the production of second-generation ethanol. For doing this, The Plackett-Burman method was used to discard variables that do not influence the enzymatic hydrolysis process, and subsequently to adjust the model using the factorial optimization design Box-Hunter 25-2. Once the conditions of the experiments in the enzymatic hydrolysis process were optimized, the best testwas selected considering the highest glucose yield per 100g bagasse. The fermentative stage was also analyzed, resulting in a possibility of producing 1 liter of second-generation ethanol per 12.83kg bagasse and 77.9 liters per ton of bagasse. |
| first_indexed | 2024-04-12T09:50:08Z |
| format | Article |
| id | doaj.art-c913029efa114f4fb49cd582ecfd2855 |
| institution | Directory Open Access Journal |
| issn | 0253-5777 2223-4861 |
| language | English |
| last_indexed | 2024-04-12T09:50:08Z |
| publishDate | 2018-09-01 |
| publisher | Universidad Central "Marta Abreu" de Las Villas |
| record_format | Article |
| series | Revista Centro Azúcar |
| spelling | doaj.art-c913029efa114f4fb49cd582ecfd28552022-12-22T03:37:50ZengUniversidad Central "Marta Abreu" de Las VillasRevista Centro Azúcar0253-57772223-48612018-09-01453101075USE OF NATIVE CELLULOLYTIC ENZYMES FROM ECUADOR, IN THE ENZYMATIC HYDROLYSIS FOR THE ETHANOLPRODUCTIONJonathan Villavicencio Montoya0Carmen Amelia Salvador Pinos1Fernando Batallas Merino2Amaury Pérez Martínez3Layanis Mesa Garriga4Erenio González Suárez5Departamento Ingeniería Química. Facultad de Química y Farmacia. Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara, CubaFacultad de Ciencias Médicas. Universidad Central del Ecuador, Av. Universitaria, Quito 170129, EcuadorDepartamento Ingeniería Química. Facultad de Química y Farmacia. Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara, CubaDepartamento de Ciencias de la Tierra. Universidad Estatal Amazónica. Troncal Amazónica E45, Puyo, EcuadorDepartamento de Bioproductos. Sede Central. Corporación Colombiana de Investigación Agropecuaria (CORPOICA). Km 14 Vía Mosquera - Bogotá, ColombiaDepartamento Ingeniería Química. Facultad de Química y Farmacia. Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara, CubaThis study shows the use of cellulolytic enzymes Bacillus sp.in enzymatic hydrolysis process and their use in the production of second-generation ethanol. For doing this, The Plackett-Burman method was used to discard variables that do not influence the enzymatic hydrolysis process, and subsequently to adjust the model using the factorial optimization design Box-Hunter 25-2. Once the conditions of the experiments in the enzymatic hydrolysis process were optimized, the best testwas selected considering the highest glucose yield per 100g bagasse. The fermentative stage was also analyzed, resulting in a possibility of producing 1 liter of second-generation ethanol per 12.83kg bagasse and 77.9 liters per ton of bagasse.http://centroazucar.uclv.edu.cu/index.php/centro_azucar/article/view/75enzyme cocktailenzyme productionsecond-generation ethanol |
| spellingShingle | Jonathan Villavicencio Montoya Carmen Amelia Salvador Pinos Fernando Batallas Merino Amaury Pérez Martínez Layanis Mesa Garriga Erenio González Suárez USE OF NATIVE CELLULOLYTIC ENZYMES FROM ECUADOR, IN THE ENZYMATIC HYDROLYSIS FOR THE ETHANOLPRODUCTION Revista Centro Azúcar enzyme cocktail enzyme production second-generation ethanol |
| title | USE OF NATIVE CELLULOLYTIC ENZYMES FROM ECUADOR, IN THE ENZYMATIC HYDROLYSIS FOR THE ETHANOLPRODUCTION |
| title_full | USE OF NATIVE CELLULOLYTIC ENZYMES FROM ECUADOR, IN THE ENZYMATIC HYDROLYSIS FOR THE ETHANOLPRODUCTION |
| title_fullStr | USE OF NATIVE CELLULOLYTIC ENZYMES FROM ECUADOR, IN THE ENZYMATIC HYDROLYSIS FOR THE ETHANOLPRODUCTION |
| title_full_unstemmed | USE OF NATIVE CELLULOLYTIC ENZYMES FROM ECUADOR, IN THE ENZYMATIC HYDROLYSIS FOR THE ETHANOLPRODUCTION |
| title_short | USE OF NATIVE CELLULOLYTIC ENZYMES FROM ECUADOR, IN THE ENZYMATIC HYDROLYSIS FOR THE ETHANOLPRODUCTION |
| title_sort | use of native cellulolytic enzymes from ecuador in the enzymatic hydrolysis for the ethanolproduction |
| topic | enzyme cocktail enzyme production second-generation ethanol |
| url | http://centroazucar.uclv.edu.cu/index.php/centro_azucar/article/view/75 |
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