Immunotherapeutic potential of n-terminally formylated ESAT-6 protein in murine tuberculosis
Background: The early secreted antigenic target-6 kDa (ESAT-6) being one of the important antigens expressed by Mycobacterium tuberculosis (MTB) has been widely investigated for its strong immunmodulatory effects. We have previously evaluated the immunotherapeutic efficacy of ESAT-6 in the murine mo...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | International Journal of Mycobacteriology |
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| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=1;spage=108;epage=112;aulast=Mir |
| _version_ | 1828105435894251520 |
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| author | Shabir Ahmad Mir Sadhna Sharma |
| author_facet | Shabir Ahmad Mir Sadhna Sharma |
| author_sort | Shabir Ahmad Mir |
| collection | DOAJ |
| description | Background: The early secreted antigenic target-6 kDa (ESAT-6) being one of the important antigens expressed by Mycobacterium tuberculosis (MTB) has been widely investigated for its strong immunmodulatory effects. We have previously evaluated the immunotherapeutic efficacy of ESAT-6 in the murine model of experimental tuberculosis (TB). Now in the present study, we have evaluated the immunotherapeutic efficacy of N-terminally formylated form of ESAT-6 (f-ESAT-6) in murine TB. Materials and Methods: The production and purification of f-ESAT-6 have been discussed in our earlier report (Mir SA and Sharma S, 2014). In the present study, the MTB H37Rv-infected mice were treated with f-ESAT-6 alone or in combination with anti-TB drugs (ATDs). Four weeks postinitiation of the treatment, the experimental mice were sacrificed, and the colony-forming units (CFUs) were enumerated in their lungs and spleen as described in “materials and methods” section. Results: The N-terminally formylated ESAT-6 protein (f-ESAT-6) induced a moderate reduction in the bacterial load in the target organs of infected mice. Compared to the dimethyldioctadecyl ammonium bromide treated and untreated groups, the f-ESAT-6 treatment significantly reduced the CFU in the spleen and lungs of infected mice by 0.377 log10 units (P < 0.05) and 0.396 log10 units (P < 0.01), respectively. The administration of f-ESAT-6 in combination with ATDs revealed an additional immunotherapeutic effect and elicited higher therapeutic efficacy over drugs (ATDs) alone. Conclusion: The results of the present study clearly indicate that f-ESAT-6 protein alone as well as in combination with the conventional ATDs induce moderate therapeutic effect against experimental TB. |
| first_indexed | 2024-04-11T10:03:20Z |
| format | Article |
| id | doaj.art-c918944e31f34afd9978b2dcb0a592c0 |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-04-11T10:03:20Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-c918944e31f34afd9978b2dcb0a592c02022-12-22T04:30:19ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2022-01-0111110811210.4103/ijmy.ijmy_39_21Immunotherapeutic potential of n-terminally formylated ESAT-6 protein in murine tuberculosisShabir Ahmad MirSadhna SharmaBackground: The early secreted antigenic target-6 kDa (ESAT-6) being one of the important antigens expressed by Mycobacterium tuberculosis (MTB) has been widely investigated for its strong immunmodulatory effects. We have previously evaluated the immunotherapeutic efficacy of ESAT-6 in the murine model of experimental tuberculosis (TB). Now in the present study, we have evaluated the immunotherapeutic efficacy of N-terminally formylated form of ESAT-6 (f-ESAT-6) in murine TB. Materials and Methods: The production and purification of f-ESAT-6 have been discussed in our earlier report (Mir SA and Sharma S, 2014). In the present study, the MTB H37Rv-infected mice were treated with f-ESAT-6 alone or in combination with anti-TB drugs (ATDs). Four weeks postinitiation of the treatment, the experimental mice were sacrificed, and the colony-forming units (CFUs) were enumerated in their lungs and spleen as described in “materials and methods” section. Results: The N-terminally formylated ESAT-6 protein (f-ESAT-6) induced a moderate reduction in the bacterial load in the target organs of infected mice. Compared to the dimethyldioctadecyl ammonium bromide treated and untreated groups, the f-ESAT-6 treatment significantly reduced the CFU in the spleen and lungs of infected mice by 0.377 log10 units (P < 0.05) and 0.396 log10 units (P < 0.01), respectively. The administration of f-ESAT-6 in combination with ATDs revealed an additional immunotherapeutic effect and elicited higher therapeutic efficacy over drugs (ATDs) alone. Conclusion: The results of the present study clearly indicate that f-ESAT-6 protein alone as well as in combination with the conventional ATDs induce moderate therapeutic effect against experimental TB.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=1;spage=108;epage=112;aulast=Miranti-tuberculosis drugscolony-forming unithistopathologyimmunotherapyn-formylated-early secreted antigenic target-6tuberculosis |
| spellingShingle | Shabir Ahmad Mir Sadhna Sharma Immunotherapeutic potential of n-terminally formylated ESAT-6 protein in murine tuberculosis International Journal of Mycobacteriology anti-tuberculosis drugs colony-forming unit histopathology immunotherapy n-formylated-early secreted antigenic target-6 tuberculosis |
| title | Immunotherapeutic potential of n-terminally formylated ESAT-6 protein in murine tuberculosis |
| title_full | Immunotherapeutic potential of n-terminally formylated ESAT-6 protein in murine tuberculosis |
| title_fullStr | Immunotherapeutic potential of n-terminally formylated ESAT-6 protein in murine tuberculosis |
| title_full_unstemmed | Immunotherapeutic potential of n-terminally formylated ESAT-6 protein in murine tuberculosis |
| title_short | Immunotherapeutic potential of n-terminally formylated ESAT-6 protein in murine tuberculosis |
| title_sort | immunotherapeutic potential of n terminally formylated esat 6 protein in murine tuberculosis |
| topic | anti-tuberculosis drugs colony-forming unit histopathology immunotherapy n-formylated-early secreted antigenic target-6 tuberculosis |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=1;spage=108;epage=112;aulast=Mir |
| work_keys_str_mv | AT shabirahmadmir immunotherapeuticpotentialofnterminallyformylatedesat6proteininmurinetuberculosis AT sadhnasharma immunotherapeuticpotentialofnterminallyformylatedesat6proteininmurinetuberculosis |